Data |
Western Blotting

Immunoprecipitation

RNP Immunoprecipitation

|
Clonality |
Polyclonal
|
Clone |
Polyclonal |
Isotype
(Immunized Animal)
|
Rabbit Ig (aff.)
|
Applications |
- WB
- 1:1,000
 - IP
- 5 µg/250 µL of cell extract from 2.5 x 106 cells
 - IC
- Can be used.
 - RIP
- 15 µg/500 µL of cell extract from 1.2 x 107 cells
 - Other(eCLIP)
- reported. (PMID: 27018577 / 27068461)
 - Other(PAR-CLIP)
- reported. (PMID: 27184842)

|
Immunogen
(Antigen) |
KLH-conjugated synthetic peptide KQQEQKYPQGVASQRSK (583-599 aa)
|
Reactivity
[Gene ID] |
Human[10644](PMID: 22899010), Mouse[319765](PMID: 22899010), Rat[303824](PMID: 22513850), Hamster(-) |
Storage buffer |
1 mg/mL in PBS/50% glycerol, pH 7.2
|
Storage temp. |
-20°C
|
Conjugate |
Unlabeled |
Manufacturer |
MBL |
Alternative names |
insulin-like growth factor 2 mRNA binding protein 2, p62, IMP-2, VICKZ2/Igf2bp2, Imp2, Neilsen, C330012H03Rik/RGD1305614
|
Background |
IGF2BP2 is an RNA binding protein that post-transcriptionally regulates IGF2 during developmental stages. IGF2 mRNA-binding protein family (IGF2BP1, 2 and 3) bind UTR region of IGF2 transcripts and are implicated to in transport of RNA targets to enable protein synthesis at specific locations in the cytoplasm. IGF2BP family members show tissue-selective expression during development. IGF2BP2 is expressed in fetal lung, kidney, thymus, placenta and liver. Genetic valuation in IGF2BP2 was implicated to interact with Fetal Malnutrition on glucose metabolism. |
Related products |
RN1001 RIP-Assay Kit RN1005 RIP-Assay Kit for microRNA RN001M Anti-IGF2BP1 (IMP1) mAb RN007P Anti-IGF2BP1 (IMP1) pAb RN009P Anti-IGF2BP3 (IMP3) pAb
|
Citations |
Western Blotting - Gong C et al. A long non-coding RNA, LncMyoD, regulates skeletal muscle differentiation by blocking IMP2-mediated mRNA translation. Dev Cell. 34, 181-91 (2015)(PMID:26143994)
- Conway AE et al. Enhanced CLIP Uncovers IMP Protein-RNA Targets in Human Pluripotent Stem Cells Important for Cell Adhesion and Survival. Cell Rep. 15, 666-79 (2016)(PMID:27068461)
- Degrauwe N et al. The RNA Binding Protein IMP2 Preserves Glioblastoma Stem Cells by Preventing let-7 Target Gene Silencing. Cell Rep. 15, 1634-47 (2016)(PMID:27184842)
- JnBaptiste CK et al. Dicer loss and recovery induce an oncogenic switch driven by transcriptional activation of the oncofetal Imp1-3 family. Genes Dev. 31, 674-687 (2017)(PMID:28446596)
- Hosono Y et al. Oncogenic Role of THOR, a Conserved Cancer/Testis Long Non-coding RNA. Cell 171, 1559-1572 (2017)(PMID:29245011)
Immunoprecipitation - Janiszewska M et al. Imp2 controls oxidative phosphorylation and is crucial for preserving glioblastoma cancer stem cells. Genes Dev. 26, 1926-44 (2012)(PMID:22899010)
- Conway AE et al. Enhanced CLIP Uncovers IMP Protein-RNA Targets in Human Pluripotent Stem Cells Important for Cell Adhesion and Survival. Cell Rep. 15, 666-79 (2016)(PMID:27068461)
- Degrauwe N et al. The RNA Binding Protein IMP2 Preserves Glioblastoma Stem Cells by Preventing let-7 Target Gene Silencing. Cell Rep. 15, 1634-47 (2016)(PMID:27184842)
- Hosono Y et al. Oncogenic Role of THOR, a Conserved Cancer/Testis Long Non-coding RNA. Cell 171, 1559-1572 (2017)(PMID:29245011)
Immunocytochemistry - Sundararaman B et al. Resources for the Comprehensive Discovery of Functional RNA Elements. Mol Cell. 61, 903-13 (2016)(PMID:26990993)
- Conway AE et al. Enhanced CLIP Uncovers IMP Protein-RNA Targets in Human Pluripotent Stem Cells Important for Cell Adhesion and Survival. Cell Rep. 15, 666-79 (2016)(PMID:27068461)
- Markmiller S et al. Context-Dependent and Disease-Specific Diversity in Protein Interactions within Stress Granules. Cell 172, 590-604.e13 (2018)(PMID:29373831)
RNP Immunoprecipitation - Janiszewska M et al. Imp2 controls oxidative phosphorylation and is crucial for preserving glioblastoma cancer stem cells. Genes Dev. 26, 1926-44 (2012)(PMID:22899010)
- Schaeffer V et al. RNA-binding protein IGF2BP2/IMP2 is required for laminin-β2 mRNA translation and is modulated by glucose concentration. Am J Physiol Renal Physiol. 303, F75-82 (2012)(PMID:22513850)
- Kim HY et al. IMP2 and IMP3 cooperate to promote the metastasis of triple-negative breast cancer through destabilization of progesterone receptor. Cancer Lett. 415, 30-39 (2018)(PMID:29217458)
Other(eCLIP) - Van Nostrand EL et al. Robust transcriptome-wide discovery of RNA-binding protein binding sites with enhanced CLIP (eCLIP). Nat Methods. 13, 508-14 (2016)(PMID:27018577)
- Conway AE et al. Enhanced CLIP Uncovers IMP Protein-RNA Targets in Human Pluripotent Stem Cells Important for Cell Adhesion and Survival. Cell Rep. 15, 666-79 (2016)(PMID:27068461)
- Van Nostrand EL et al. CRISPR/Cas9-mediated integration enables TAG-eCLIP of endogenously tagged RNA binding proteins. Methods. 118-119, 50-59 (2017)(PMID:28003131)
Other(PAR-CLIP) - Degrauwe N et al. The RNA Binding Protein IMP2 Preserves Glioblastoma Stem Cells by Preventing let-7 Target Gene Silencing. Cell Rep. 15, 1634-47 (2016)(PMID:27184842)
|
Product category |
- Research area
- RNA-RNP Network
- Brands
- RiboCluster Profiler™
|