Data |
Western Blotting

Immunoprecipitation

Immunocytochemistry

Immunohistochemistry

|
Clonality |
Polyclonal
|
Clone |
Polyclonal |
Isotype
(Immunized Animal)
|
Rabbit Ig (aff.)
|
Applications |
- WB
- 1:500
 - IP
- 2 µL/sample
- IC
- 1:500
 - IH
- 1:1,000

|
Immunogen
(Antigen) |
KLH conjugated synthetic peptide, CKEVDP(pS)TGELQSLQ (corresponding to amino acid residues 346-359 of mouse p62 (SQSTM1))
|
Reactivity
[Gene ID] |
Human[8878], Mouse[18412] |
Storage buffer |
PBS/50% glycerol, pH 7.2
|
Storage temp. |
-20°C
|
Conjugate |
Unlabeled |
Manufacturer |
MBL |
Alternative names |
Sequestosome-1, Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa, Ubiquitin-binding protein p62, EBI3-associated protein of 60 kDa, p60, EBIAP, SQSTM1, ORCA, OSIL
|
Related products |
D343-3 Anti-Phospho-p62 (SQSTM1) (Ser403) mAb D344-3 Anti-Phospho-p62 (SQSTM1) (Ser403) mAb PM045 Anti-p62 (SQSTM1) pAb M162-3 Anti-p62 (SQSTM1) (Human) mAb M162-A48 Anti-p62 (SQSTM1) (Human) mAb-Alexa Fluor™ 488 M162-A59 Anti-p62 (SQSTM1) (Human) mAb-Alexa Fluor™ 594 M162-A64 Anti-p62 (SQSTM1) (Human) mAb-Alexa Fluor™ 647 PM066 Anti-p62 C-terminal pAb 8485 Autophagy Ab Sampler Set D343-3MS Anti-Phospho-p62 (SQSTM1) (Ser403) mAb for My select sampler set D344-3MS Anti-Phospho-p62 (SQSTM1) (Ser403) mAb for My select sampler set M230-3 Anti-Parkin mAb 8486 Autophagy Watch M217-3 Anti-Phospho-p62 (SQSTM1) (Ser351) mAb M217-3MS Anti-Phospho-p62 (SQSTM1) (Ser351) mAb for My select sampler set M224-3 Anti-KEAP1 mAb CY-7055 CycLex® Total p62 ELISA Kit
|
Citations |
Western Blotting - Johansson I et al. The marine n-3 PUFA DHA evokes cytoprotection against oxidative stress and protein misfolding by inducing autophagy and NFE2L2 in human retinal pigment epithelial cells. Autophagy 11, 1636-51 (2015)(PMID:26237736)
- Yoshii SR et al. Systemic Analysis of Atg5-Null Mice Rescued from Neonatal Lethality by Transgenic ATG5 Expression in Neurons. Dev Cell 39, 116-130 (2016)(PMID:27693508)
- Watanabe Y et al. HSF1 stress response pathway regulates autophagy receptor SQSTM1/p62-associated proteostasis. Autophagy 13, 133-148 (2017)(PMID:27846364)
- Yanagisawa H et al. L-leucine and SPNS1 coordinately ameliorate dysfunction of autophagy in mouse and human Niemann-Pick type C disease. Sci Rep. 7, 15994 (2017)(PMID:29162837)
- Mizunoe Y et al. Trehalose protects against oxidative stress by regulating the Keap1-Nrf2 and autophagy pathways. Redox Biol. 15, 115-124 (2017)(PMID:29241092)
- Bak DH et al. Anti‑apoptotic effects of human placental hydrolysate against hepatocyte toxicity in vivo and in vitro. Int J Mol Med. 42, 2569-2583 (2018)(PMID:30132515)
- Jena KK et al. TRIM16 controls assembly and degradation of protein aggregates by modulating the p62‐NRF2 axis and autophagy. EMBO J. 37, e98358 (2018)(PMID:30143514)
- Yamada M et al. p62/SQSTM1 and Nrf2 are essential for exercise-mediated enhancement of antioxidant protein expression in oxidative muscle. FASEB J. 33, 8022-8032 (2019)
(PMID:30913396)
- Wang Y et al. Activation of PINK1-Parkin-dependent mitophagy in Tri-ortho-cresyl phosphate-treated Neuro2a cells. Chem Biol Interact. 308, 70-79 (2019)(PMID:31100276)
- Park JY et al. TFEB activates Nrf2 by repressing its E3 ubiquitin ligase DCAF11 and promoting phosphorylation of p62. Sci Rep. 9, 14354 (2019)(PMID:31586112)
Immunocytochemistry - Watanabe Y et al. HSF1 stress response pathway regulates autophagy receptor SQSTM1/p62-associated proteostasis. Autophagy 13, 133-148 (2017)(PMID:27846364)
Immunohistochemistry - Watanabe Y et al. HSF1 stress response pathway regulates autophagy receptor SQSTM1/p62-associated proteostasis. Autophagy 13, 133-148 (2017)(PMID:27846364)
|
References |
- Ichimura Y et al. Phosphorylation of p62 activates the Keap1-Nrf2 pathway during selective autophagy. Mol Cell 51, 618-31 (2013)(PMID: 24011591)
- Kageyama S et al. Proteasome dysfunction activates autophagy and the keap1-nrf2 pathway. J Biol Chem. 289, 24944-55 (2014)(PMID: 25049227)
|
Product category |
- Research area
- Autophagy
|