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HOME > Product search results > Code No. PM044 Anti-PIWIL2 (MILI) (Mouse) pAb

Code No.　PM044

Anti-PIWIL2 (MILI) (Mouse) pAb

Price

¥52,800

Availability (in Japan)

10 or more

（In Japan at 17:00,
Apr 23, 2024 in JST）

Size

100 µL

Data

  • Western Blotting
Clonality Polyclonal Clone Polyclonal
Isotype (Immunized Animal) Rabbit Ig (aff.)
Applications
WB
1:1,000  
IP
1 µL/sample  
IH*
reported.  （PMID: 29018194）
RIP*
reported.  （PMID: 18381894 / 19735482 / 20439430）
Immunogen (Antigen) synthetic peptide VRKDREEPRSSLPDPS corresponding to Mouse PIWIL2 (107-122 a.a.)
Reactivity [Gene ID]

Mouse[57746]
Storage buffer PBS/50% glycerol, pH 7.2
Storage temp. -20°C Conjugate Unlabeled Manufacturer MBL
Background The Piwi/Argonaute protein family plays important roles in stem-cell self-renewal, RNA silencing and translational regulation in various organisms. The family members are defined by conserved both PAZ (Piwi Argonaut and Zwille) and Piwi domains participate in RNA binding. Piwi-interacting RNA (piRNA) is a class of small RNA molecules that is expressed uniquely in mammalian testes and forms RNA-protein complexes with Piwi proteins. These small regulatory RNAs have been implicated in spermatogenesis, repression of retrotransposon transposition in germline cells, epigenetic regulation and positive regulation of translation and mRNA stability. MIWI (PIWIL1) and MILI (PIWIL2) are mouse homologues of Piwi. MILI is expressed from primordial germ cells (PGC) in embryo, whereas MIWI is detected later from the mid-pachytene stage to early round spermatids. Their expression overlaps in the mid-pachytene and early round spermatid stage. The expression patterns and knockout phenotypes of mammalian Piwi members indicated MILI is an essential factor for meiotic differentiation during spermatogenesis.
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Citations

Western Blotting

  1. Kojima K et al. Associations between PIWI proteins and TDRD1/MTR-1 are critical for integrated subcellular localization in murine male germ cells. Genes Cells. 14, 1155-65 (2009)(PMID:19735482)
  2. Kuramochi-Miyagawa S et al. MVH in piRNA processing and gene silencing of retrotransposons. Genes Dev. 24, 887-92 (2010)(PMID:20439430)
  3. Ding D et al. PNLDC1 is essential for piRNA 3' end trimming and transposon silencing during spermatogenesis in mice. Nat Commun. 8, 819 (2017)(PMID:29018194)
  4. Ding D et al. TDRD5 binds piRNA precursors and selectively enhances pachytene piRNA processing in mice. Nat Commun. 9, 127 (2017)(PMID:29317670)
  5. Ding D et al. Mitochondrial membrane-based initial separation of MIWI and MILI functions during pachytene piRNA biogenesis. Nucleic Acids Res. 47, 2594-2608 (2019) (PMID:30590800)

Immunoprecipitation

  1. Kojima K et al. Associations between PIWI proteins and TDRD1/MTR-1 are critical for integrated subcellular localization in murine male germ cells. Genes Cells. 14, 1155-65 (2009)(PMID:19735482)
  2. Shiromoto Y et al. GPAT2, a mitochondrial outer membrane protein, in piRNA biogenesis in germline stem cells. RNA 19, 803-10 (2013)(PMID:23611983)

Immunohistochemistry

  1. Ding D et al. PNLDC1 is essential for piRNA 3' end trimming and transposon silencing during spermatogenesis in mice. Nat Commun. 8, 819 (2017)(PMID:29018194)
  2. Ding D et al. TDRD5 binds piRNA precursors and selectively enhances pachytene piRNA processing in mice. Nat Commun. 9, 127 (2017)(PMID:29317670)
  3. Ding D et al. Mitochondrial membrane-based initial separation of MIWI and MILI functions during pachytene piRNA biogenesis. Nucleic Acids Res. 47, 2594-2608 (2019)(PMID:30590800)

RNP Immunoprecipitation

  1. Kuramochi-Miyagawa S et al. DNA methylation of retrotransposon genes is regulated by Piwi family members MILI and MIWI2 in murine fetal testes. Genes Dev. 22, 908-17 (2008)(PMID:18381894)
  2. Kojima K et al. Associations between PIWI proteins and TDRD1/MTR-1 are critical for integrated subcellular localization in murine male germ cells. Genes Cells. 14, 1155-65 (2009)(PMID:19735482)
  3. Kuramochi-Miyagawa S et al. MVH in piRNA processing and gene silencing of retrotransposons. Genes Dev. 24, 887-92 (2010)(PMID:20439430)
  4. Ding D et al. PNLDC1 is essential for piRNA 3' end trimming and transposon silencing during spermatogenesis in mice. Nat Commun. 8, 819 (2017)(PMID:29018194)
  5. Ding D et al. TDRD5 binds piRNA precursors and selectively enhances pachytene piRNA processing in mice. Nat Commun. 9, 127 (2017)(PMID:29317670)
  6. Nishimura T et al. PNLDC1, mouse pre-piRNA Trimmer, is required for meiotic and post-meiotic male germ cell development. EMBO Rep. 19, e44957 (2018)(PMID:29444933)
  7. Ding D et al. Mitochondrial membrane-based initial separation of MIWI and MILI functions during pachytene piRNA biogenesis. Nucleic Acids Res. 47, 2594-2608 (2019)(PMID:30590800)
Product category
Research area
Stem cell research
RNA-RNP Network
Epigenetics
Cancer
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  • The availability is based on the information in Japan at 17:00, Apr 23, 2024 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications：
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
    DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.