Anti-S-tag pAb

Price

¥35,200

Availability (in Japan)

1-9

（In Japan at 17:00,
Jan 18, 2025 in JST）

Size

100 µL

Citations

Data Sheet

SDS

Data	Immunoprecipitation
Clonality	Polyclonal	Clone	Polyclonal
Isotype (Immunized Animal)	Rabbit Ig (aff.)
Applications	WB 1:1,000 IP 5 µL/sample
Immunogen (Antigen)	Carrier protein (CP) conjugated synthetic peptide (CP-KETAAAKFERQHIDSC)
Storage buffer	PBS/50% glycerol, pH 7.2
Storage temp.	-20°C	Conjugate	Unlabeled	Manufacturer	MBL
Background	Epitope tagging is a powerful and versatile strategy for detecting and purifying proteins expressed by cloned genes. Short sequences encoding the epitope tag are cloned in-frame with target DNA to produce fusion proteins containing the epitope tag peptide. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Anti-epitope tag antibodies can serve as universal purification or detection reagents for any tag-containing protein. S-tag is an epitope tag composed of a 15 residues peptide, KETAAAKFERQHMDS, derived from the pancreatic ribonuclease A. S-tag can combine with S-protein to form functional RNase S, allowing detection and quantification of S-tag fusion proteins by enzymatic assays. However, antibody against the S-tag sequence provides a more convenient and flexible method to easily identify, detect, or purify S-tag containing fusion proteins.
Citations	Western Blotting Ogawa D et al. Molecular characterization of the rice protein RSS1 required for meristematic activity under stressful conditions. Plant Physiol. Biochem. 61, 54-60 (2012)(PMID:23041461) O'Rourke TW et al. Amyloid-like assembly of the low complexity domain of yeast Nab3. Prion 9, 34-47 (2015)(PMID:25611193) O'Rourke TW, Reines D. Determinants of Amyloid Formation for the Yeast Termination Factor Nab3. PLoS One 11, e0150865 (2016)(PMID:26954508) Zhao J et al. GtfA Interacting with GtfB is Required for PsrP Glycosylation in Streptococcus pneumoniae. Jundishapur J Microbiol. 11, e68982 (2018) Kadooka C et al. Mitochondrial citrate transporters CtpA and YhmA are required for extracellular citric acid accumulation and contribute to cytosolic acetyl-CoA generation in Aspergillus luchuensis mut. kawachii. Appl Environ Microbiol. (2019) In press.(PMID:30737343) Hosokawa K et al. The Lysosome Pathway Degrades CD81 on the Cell Surface by Poly-ubiquitination and Clathrin-Mediated Endocytosis. Biol Pharm Bull. 43, 540-545 (2020)(PMID:31902824) Immunoprecipitation Hosokawa K et al. The Lysosome Pathway Degrades CD81 on the Cell Surface by Poly-ubiquitination and Clathrin-Mediated Endocytosis. Biol Pharm Bull. 43, 540-545 (2020)(PMID:31902824)
Product category	Tools Epitope tags

The availability is based on the information in Japan at 17:00, Jan 18, 2025 in JST.
The special price is shown in red color.
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Abbreviations for applications：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
For applications and reactivity:
*: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
**: The use is reported from the licenser (Under evaluation or not tested by MBL).
For storage temparature: RT: room temparature
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