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HOME > Product search results > Code No. PM021 Anti-S-tag pAb

Code No.　PM021

Anti-S-tag pAb

Price

¥35,200

Availability (in Japan)

1-9

（In Japan at 17:00,
Apr 19, 2024 in JST）

Size

100 µL

Data

  • Immunoprecipitation
Clonality Polyclonal Clone Polyclonal
Isotype (Immunized Animal) Rabbit Ig (aff.)
Applications
WB
1:1,000  
IP
5 µL/sample  
Immunogen (Antigen) Carrier protein (CP) conjugated synthetic peptide (CP-KETAAAKFERQHIDSC)
Storage buffer PBS/50% glycerol, pH 7.2
Storage temp. -20°C Conjugate Unlabeled Manufacturer MBL
Background Epitope tagging is a powerful and versatile strategy for detecting and purifying proteins expressed by cloned genes. Short sequences encoding the epitope tag are cloned in-frame with target DNA to
produce fusion proteins containing the epitope tag peptide. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Anti-epitope tag antibodies can serve as universal purification or detection reagents for any tag-containing protein. S-tag is an epitope tag composed of a 15 residues peptide, KETAAAKFERQHMDS, derived from the pancreatic ribonuclease A. S-tag can combine with S-protein to form functional RNase S, allowing detection and quantification of S-tag fusion proteins by enzymatic assays. However, antibody against the S-tag sequence provides a more convenient and flexible method to easily identify, detect, or purify S-tag containing fusion proteins.
Citations

Western Blotting

  1. Ogawa D et al. Molecular characterization of the rice protein RSS1 required for meristematic activity under stressful conditions. Plant Physiol. Biochem. 61, 54-60 (2012)(PMID:23041461)
  2. O'Rourke TW et al. Amyloid-like assembly of the low complexity domain of yeast Nab3. Prion 9, 34-47 (2015)(PMID:25611193)
  3. O'Rourke TW, Reines D. Determinants of Amyloid Formation for the Yeast Termination Factor Nab3. PLoS One 11, e0150865 (2016)(PMID:26954508)
  4. Zhao J et al. GtfA Interacting with GtfB is Required for PsrP Glycosylation in Streptococcus pneumoniae. Jundishapur J Microbiol. 11, e68982 (2018)
  5. Kadooka C et al. Mitochondrial citrate transporters CtpA and YhmA are required for extracellular citric acid accumulation and contribute to cytosolic acetyl-CoA generation in Aspergillus luchuensis mut. kawachii. Appl Environ Microbiol. (2019) In press.(PMID:30737343)
  6. Hosokawa K et al. The Lysosome Pathway Degrades CD81 on the Cell Surface by Poly-ubiquitination and Clathrin-Mediated Endocytosis. Biol Pharm Bull. 43, 540-545 (2020)(PMID:31902824)

Immunoprecipitation

  1. Hosokawa K et al. The Lysosome Pathway Degrades CD81 on the Cell Surface by Poly-ubiquitination and Clathrin-Mediated Endocytosis. Biol Pharm Bull. 43, 540-545 (2020)(PMID:31902824)
Product category
Tools
Epitope tags
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  • The availability is based on the information in Japan at 17:00, Apr 19, 2024 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications：
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
    DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.