Data |
Western Blotting

Immunoprecipitation

Immunocytochemistry

|
Clonality |
Polyclonal
|
Clone |
Polyclonal |
Isotype
(Immunized Animal)
|
Guinea pig IgG
|
Applications |
- WB
- 1:1,000
 - IP
- 2-5 µL/300 µL of cell extract from 3 x 106 cells
- IC
- 1:1,000

|
Immunogen
(Antigen) |
Recombinant Human CENP-C N-terminal (1-426 aa)
|
Reactivity
[Gene ID] |
Human[1060] |
Storage buffer |
PBS/50% glycerol, pH 7.2
|
Storage temp. |
-20°C
|
Conjugate |
Unlabeled |
Manufacturer |
MBL |
Alternative names |
CENPC, MIF2, hcp-4
|
Background |
Centromere protein C (CENP-C/ICEN7) is a 140 kDa protein and a component of the centromere specific chromatin complex called CENP-A chromatin or ICEN in interphase and forms basis for the inner-kinetochore plate in metaphase. CENP-C is essential for the kinetochore function of equal segregation of daughter chromosomes. |
Related products |
PM067 Normal Guinea Pig IgG D115-3 Anti-CENP-A (Human) mAb M146-3 Anti-Nuf2 (Human) mAb
|
Citations |
Western Blotting - Bui M et al. Internal modifications in the CENP-A nucleosome modulate centromeric dynamics. Epigenetics Chromatin. 10, 17 (2017)(PMID:28396698)
Immunocytochemistry - Kuijt TE et al. Conditional targeting of MAD1 to kinetochores is sufficient to reactivate the spindle assembly checkpoint in metaphase. Chromosoma (2014) In press.(PMID:24695965)
- Nijenhuis W et al. Negative feedback at kinetochores underlies a responsive spindle checkpoint signal. Nat Cell Biol. 16, 1257-64 (2014)(PMID:25402682)
- Etemed B et al. Kinetochore-microtubule attachment is sufficient to satisfy the human spindle assembly checkpoint. Nat Commun. 6, 8987 (2015)(PMID:26621779)
- Ly P et al. Selective Y centromere inactivation triggers chromosome shattering in micronuclei and repair by non-homologous end joining. Nat Cell Biol. 19, 68-75 (2017)(PMID:27918550)
- Bui M et al. Internal modifications in the CENP-A nucleosome modulate centromeric dynamics. Epigenetics Chromatin. 10, 17 (2017)(PMID:28396698)
- Hengeveld RCC et al. Inner centromere localization of the CPC maintains centromere cohesion and allows mitotic checkpoint silencing. Nat Commun. 8, 15542 (2017)(PMID:28561035)
- Haase J et al. Distinct Roles of the Chromosomal Passenger Complex in the Detection of and Response to Errors in Kinetochore-Microtubule Attachment. Dev Cell. 42, 640-654.e5 (2017)(PMID:28950102)
- Liang C et al. A kinase-dependent role for Haspin in antagonizing Wapl and protecting mitotic centromere cohesion. EMBO Rep. 19, 43-56 (2018)(PMID:29138236)
- Combes G et al. Mps1 Phosphorylates Its N-Terminal Extension to Relieve Autoinhibition and Activate the Spindle Assembly Checkpoint. Curr Biol. 28, 872-883.e5 (2018)(PMID:29502948)
- Soto M et al. Chromosomes trapped in micronuclei are liable to segregation errors. J Cell Sci. 131, jcs214742 (2018)(PMID:29930083)
- Pereira C et al. Self-Assembly of the RZZ Complex into Filaments Drives Kinetochore Expansion in the Absence of Microtubule Attachment. Curr Biol. 28, 3408-3421.e8 (2018)(PMID:30415699)
- Liang C et al. A positive feedback mechanism ensures proper assembly of the functional inner centromere during mitosis in human cells. J Biol Chem. (20189 In press.(PMID:30498087)
- Yi Q et al. Aurora B kinase activity-dependent and -independent functions of the chromosomal passenger complex in regulating sister chromatid cohesion. J Biol Chem. (2018) In press.(PMID:30523151)
Immunofluorescence - Watanabe K et al. The Cep57-pericentrin module organizes PCM expansion and centriole engagement. Nat Commun. 10, 931 (2019)(PMID:30804344)
- Venegas AB et al. Inducible Degradation of the Human SMC5/6 Complex Reveals an Essential Role Only during Interphase. Cell Rep. 31, 107533 (2020)(PMID:32320646)
- Asai Y et al. SET/TAF1 forms a distance-dependent feedback loop with Aurora B and Bub1 as a tension sensor at centromeres. Sci Rep. 10, 15653 (2020)(PMID:32973131)
|
Product category |
- Research area
- Epigenetics
Cancer
|