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HOME > Product search results > Code No. PD030 Anti-CENP-C (Human) pAb

Code No.　PD030

Anti-CENP-C (Human) pAb

Price

¥48,000

Availability (in Japan)

10 or more

（In Japan at 17:00,
Jul 1, 2022 in JST）

Size

100 µL

Data

  • Western Blotting

  • Immunoprecipitation

  • Immunocytochemistry
Clonality Polyclonal Clone Polyclonal
Isotype (Immunized Animal) Guinea pig IgG
Applications
WB
1:1,000  
IP
2-5 µL/300 µL of cell extract from 3 x 106 cells  
IC
1:1,000  
Immunogen (Antigen) Recombinant Human CENP-C N-terminal (1-426 aa)
Reactivity [Gene ID]

Human[1060]
Storage buffer PBS/50% glycerol, pH 7.2
Storage temp. -20°C Conjugate Unlabeled Manufacturer MBL
Alternative names CENPC, MIF2, hcp-4
Background Centromere protein C (CENP-C/ICEN7) is a 140 kDa protein and a component of the centromere specific chromatin complex called CENP-A chromatin or ICEN in interphase and forms basis for the inner-kinetochore plate in metaphase. CENP-C is essential for the kinetochore function of equal segregation of daughter chromosomes.
Related products PM067 Normal Guinea Pig IgG
D115-3 Anti-CENP-A (Human) mAb
M146-3 Anti-Nuf2 (Human) mAb
Citations

Western Blotting

  1. Bui M et al. Internal modifications in the CENP-A nucleosome modulate centromeric dynamics. Epigenetics Chromatin. 10, 17 (2017)(PMID:28396698)

Immunocytochemistry

  1. Kuijt TE et al. Conditional targeting of MAD1 to kinetochores is sufficient to reactivate the spindle assembly checkpoint in metaphase. Chromosoma (2014) In press.(PMID:24695965)
  2. Nijenhuis W et al. Negative feedback at kinetochores underlies a responsive spindle checkpoint signal. Nat Cell Biol. 16, 1257-64 (2014)(PMID:25402682)
  3. Etemed B et al. Kinetochore-microtubule attachment is sufficient to satisfy the human spindle assembly checkpoint. Nat Commun. 6, 8987 (2015)(PMID:26621779)
  4. Ly P et al. Selective Y centromere inactivation triggers chromosome shattering in micronuclei and repair by non-homologous end joining. Nat Cell Biol. 19, 68-75 (2017)(PMID:27918550)
  5. Bui M et al. Internal modifications in the CENP-A nucleosome modulate centromeric dynamics. Epigenetics Chromatin. 10, 17 (2017)(PMID:28396698)
  6. Hengeveld RCC et al. Inner centromere localization of the CPC maintains centromere cohesion and allows mitotic checkpoint silencing. Nat Commun. 8, 15542 (2017)(PMID:28561035)
  7. Haase J et al. Distinct Roles of the Chromosomal Passenger Complex in the Detection of and Response to Errors in Kinetochore-Microtubule Attachment. Dev Cell. 42, 640-654.e5 (2017)(PMID:28950102)
  8. Liang C et al. A kinase-dependent role for Haspin in antagonizing Wapl and protecting mitotic centromere cohesion. EMBO Rep. 19, 43-56 (2018)(PMID:29138236)
  9. Combes G et al. Mps1 Phosphorylates Its N-Terminal Extension to Relieve Autoinhibition and Activate the Spindle Assembly Checkpoint. Curr Biol. 28, 872-883.e5 (2018)(PMID:29502948)
  10. Soto M et al. Chromosomes trapped in micronuclei are liable to segregation errors. J Cell Sci. 131, jcs214742 (2018)(PMID:29930083)
  11. Pereira C et al. Self-Assembly of the RZZ Complex into Filaments Drives Kinetochore Expansion in the Absence of Microtubule Attachment. Curr Biol. 28, 3408-3421.e8 (2018)(PMID:30415699)
  12. Liang C et al. A positive feedback mechanism ensures proper assembly of the functional inner centromere during mitosis in human cells. J Biol Chem. (20189 In press.(PMID:30498087)
  13. Yi Q et al. Aurora B kinase activity-dependent and -independent functions of the chromosomal passenger complex in regulating sister chromatid cohesion. J Biol Chem. (2018) In press.(PMID:30523151)

Immunofluorescence

  1. Watanabe K et al. The Cep57-pericentrin module organizes PCM expansion and centriole engagement. Nat Commun. 10, 931 (2019)(PMID:30804344)
  2. Venegas AB et al. Inducible Degradation of the Human SMC5/6 Complex Reveals an Essential Role Only during Interphase. Cell Rep. 31, 107533 (2020)(PMID:32320646)
  3. Asai Y et al. SET/TAF1 forms a distance-dependent feedback loop with Aurora B and Bub1 as a tension sensor at centromeres. Sci Rep. 10, 15653 (2020)(PMID:32973131)
Product category
Research area
Epigenetics
Cancer
この製品に関するお問い合わせをする

  • The availability is based on the information in Japan at 17:00, Jul 1, 2022 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications：
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
    DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.