Data |
Western Blotting
Immunoprecipitation
Immunocytochemistry
Immunohistochemistry
Immunohistochemistry
|
Clonality |
Polyclonal
|
Clone |
Polyclonal |
Isotype
(Immunized Animal)
|
Rabbit IgG
|
Applications |
- WB
- 1:2,000
- IP
- 1 µL/150 µg of cell lysate
- IC
- 1:500
- IH
- 1:2,500-1:5,000
- IF
- 1:500
|
Immunogen
(Antigen) |
recombinant region of human ATBF1 (2107-2147 aa.) corresponding to the identical amino acid sequence of mouse ATBF1 (2114-2154 aa.)
|
Reactivity
[Gene ID] |
Human[463], Mouse[11906], Rat[307829] |
Storage buffer |
PBS/50% glycerol, pH 7.2
|
Storage temp. |
-20°C
|
Conjugate |
Unlabeled |
Manufacturer |
MBL |
Alternative names |
ZFHX3, ATBT, ZNF927
|
Background |
ATBF1 (also known as ZFHX3) is a transcription factor that has two-protein isoforms, the 404 kDa ATBF1-A and the 306 kDa ATBF1-B. ATBF1-A contains four homeodomains and 23 zinc-finger motifs. ATBF1-B contains four homeodomains and 18 zinc fingers. ATBF1 is identified as DNA-binding protein, which binds to an AT-rich element of the human α-fetoprotein (AFP) gene, as a result suppressing its transcription activity. ATBF1 is also involved in cell cycle arrest and cooperating with p53 to activate the p21Waf1/Cip1 promoter. ATBF1 is expressed in the differentiation fields in association with β-tubulin III and MAP2 that are the neuronal differentiation marker. ATBF1 plays a crucial role in neuronal development and cell cycle arrest. |
Related products |
PD011 Anti-ATBF1 (AT-6) pAb
|
Citations |
Western Blotting - Zhang S et al. AT motif binding factor 1 (ATBF1) is highly phosphorylated in embryonic brain and protected from cleavage by calpain-1. Biochem Biophys Res Commun. 427, 537-41 (2012)(PMID:23022192)
- Chen XH et al. Mebendazole elicits potent antimyeloma activity by inhibiting the USP5/c-Maf axis. Acta Pharmacol Sin. 40, 1568-1577 (2019)(PMID:31152485)
- Zhang Z et al. Zfhx3 is required for the differentiation of late born D1-type medium spiny neurons. Exp Neurol. 322, 113055 (2019)(PMID:31491374)
Immunocytochemistry - Kawaguchi M et al. A diagnostic marker for superficial urothelial bladder carcinoma: lack of nuclear ATBF1 (ZFHX3) by immunohistochemistry suggests malignant progression. BMC Cancer 16, 805 (2016)(PMID:27756245)
Immunohistochemistry - Kim TS et al. The ZFHX3 (ATBF1) transcription factor induces PDGFRB, which activates ATM in the cytoplasm to protect cerebellar neurons from oxidative stress. Dis Model Mech. 3, 752-62 (2010)(PMID:20876357)
- Uhm KO et al. ATBF1 is a novel amyloid-β protein precursor (AβPP) binding protein that affects AβPP expression. J Alzheimers Dis. 43, 243-57 (2015)(PMID:25079792)
- Zhang Z et al. Zfhx3 is required for the differentiation of late born D1-type medium spiny neurons. Exp Neurol. 322, 113055 (2019)(PMID:31491374)
- Dong G et al. ZFHX3 Promotes the Proliferation and Tumor Growth of ER-Positive Breast Cancer Cells Likely by Enhancing Stem-Like Features and MYC and TBX3 Transcription. Cancers (Basel). 12, 3415 (2020)(PMID:33217982)
Immunofluorescence - Jung CG et al. Homeotic factor ATBF1 induces the cell cycle arrest associated with neuronal differentiation. Development 132, 5137-45 (2005)(PMID:16251211)
|
Product category |
- Research area
- Cancer
|