Data |
Immunoprecipitation

|
Clonality |
Monoclonal
|
Clone |
4E12 |
Isotype
(Immunized Animal)
|
Mouse IgG1 κ
|
Applications |
- WB*
- Not recommended. Code No. M186-3, M186-7 and PM036 are more suitable for Western blotting.
 - IP
- 5 µg/300 µL of cell extract from 1x107 cells
 - FCM
- 40 µg/mL
- IC
- 40 µg/mL
 - IH*
- reported. (PMID: 21957238 / 19955365 / 19915056)
 - Other(Immuno-EM)
- 20 µg/mL (PMID: 24553140)

|
Immunogen
(Antigen) |
Recombinant Human LC3 (MAP1LC3B: 1-120 a.a.)
|
Reactivity
[Gene ID] |
Human[81631/84557], Mouse[67443/66734], Rat[64862/362245], Hamster[100760576/100769810] |
Storage buffer |
2 mg/mL in PBS/50% glycerol, pH 7.2
|
Storage temp. |
-20°C
|
Conjugate |
Unlabeled |
Manufacturer |
MBL |
Alternative names |
microtubule-associated protein 1 light chain 3 beta, microtubule-associated protein 1 light chain 3 alpha, LC3B, ATG8F, MAP1LC3B-a, MAP1A/1BLC3, MAP1LC3B, LC3A, ATG8E, MAP1ALC3, MAP1BLC3, MAP1LC3A
|
Background |
Macroautophagy mediates the bulk degradation of cytoplasmic components. These components are delivered to lysosomes via autophagosomes. The rat microtubule-associated protein 1 light chain 3 (LC3), a homologue of yeast Atg8 (Aut7/Apg8), localizes to autophagosomal membranes after post-translational modifications. The C-terminal fragment of LC3 is cleaved immediately following synthesis to yield a cytosolic form called LC3-I. A subpopulation of LC3-I is further converted to an autophagosome-associating form, LC3-II. This antibody can detect both forms of LC3. |
Related products |
M135-3 Anti-GABARAP mAb PM038 Anti-GATE-16 pAb PM039 Anti-Atg7 (Human) pAb 8485 Autophagy Ab Sampler Set PM036-PN Positive control for anti-LC3 antibody M133-3 Anti-Atg3 mAb PM036 Anti-LC3 pAb M186-3 Anti-LC3 mAb PD014 Anti-LC3 pAb PM072 Anti-VMP1 pAb PM045 Anti-p62 (SQSTM1) pAb M162-3 Anti-p62 (SQSTM1) (Human) mAb PM066 Anti-p62 C-terminal pAb PD017 Anti-Beclin 1 pAb PM037 Anti-GABARAP pAb 8486 Autophagy Watch
|
Citations |
Western Blotting - Ho H et al. WIPI1 coordinates melanogenic gene transcription and melanosome formation via TORC1 inhibition. J Biol Chem. 286, 12509-23 (2011)(PMID:21317285)
- Saiki S et al. Caffeine induces apoptosis by enhancement of autophagy via PI3K/Akt/mTOR/p70S6K inhibition. Autophagy 7, 176-187 (2011)(PMID:21081844)
- Kasai M et al. Autophagic compartments gain access to the MHC class II compartments in thymic epithelium. J Immunol. 183, 7278-85 (2009)(PMID:19915056)
- Wang Y et al. Loss of macroautophagy promotes or prevents fibroblast apoptosis depending on the death stimulus. J Biol Chem. 283, 4766-77 (2008)(PMID:18073215)
- Murthy A et al. A Crohn's disease variant in Atg16l1 enhances its degradation by caspase 3. Nature 506, 456-62 (2014)(PMID:24553140)
Immunoprecipitation - Mookerjee S et al. Posttranslational modification of ataxin-7 at lysine 257 prevents autophagy-mediated turnover of an N-terminal caspase-7 cleavage fragment. J Neurosci. 29, 15134-44 (2009)(PMID:19955365)
Immunocytochemistry - Kasai M et al. Autophagic compartments gain access to the MHC class II compartments in thymic epithelium. J Immunol. 183, 7278-85 (2009)(PMID:19915056)
- Nishitsuji K et al. The E693Delta mutation in amyloid precursor protein increases intracellular accumulation of amyloid beta oligomers and causes endoplasmic reticulum stress-induced apoptosis in cultured cells. Am J Pathol. 174, 957-69 (2009)(PMID:19164507)
- Wang Y et al. Loss of macroautophagy promotes or prevents fibroblast apoptosis depending on the death stimulus. J Biol Chem. 283, 4766-77 (2008)(PMID:18073215)
- Chalazonitis A et al. Homeodomain interacting protein kinase 2 regulates postnatal development of enteric dopaminergic neurons and glia via BMP signaling. J Neurosci. 31, 13746-57 (2011)(PMID:21957238)
- Mori H et al. Induction of selective autophagy in cells replicating hepatitis C virus genome. J Gen Virol. 99, 1643-1657 (2018)(PMID:30311874)
Immunohistochemistry - Kasai M et al. Autophagic compartments gain access to the MHC class II compartments in thymic epithelium. J Immunol. 183, 7278-85 (2009)(PMID:19915056)
- Mookerjee S et al. Posttranslational modification of ataxin-7 at lysine 257 prevents autophagy-mediated turnover of an N-terminal caspase-7 cleavage fragment. J Neurosci. 29, 15134-44 (2009)(PMID:19955365)
Other(Immuno-EM) - Murthy A et al. A Crohn's disease variant in Atg16l1 enhances its degradation by caspase 3. Nature 506, 456-62 (2014)(PMID:24553140)
|
Product category |
- Research area
- Autophagy
|