Anti-Atg3 mAb for My select sampler set

Price

¥12,000

Availability (in Japan)

1-9

（In Japan at 00:05,
Jul 30, 2021 in JST）

Size

20 µg/20 µL

Data	Western Blotting Immunoprecipitation
Clonality	Monoclonal	Clone	3E8
Isotype (Immunized Animal)	Mouse IgG2bκ
Applications	WB 1 μg/mL IP 2.5 µg/300 µL of cell extract from 3x10⁶ cells IC 0.5 μg/mL
Immunogen (Antigen)	Recombinant Human Atg3
Reactivity [Gene ID]	Human[64422], Mouse[67841], Rat[171415], Hamster
Storage buffer	1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp.	-20°C	Conjugate	Unlabeled	Manufacturer	MBL
Alternative names	autophagy related 3, ATG3, APG3, APG3L, PC3-96, APG3-LIKE
Citations	Western Blotting Otomo C et al. Structure of the human ATG12~ATG5 conjugate required for LC3 lipidation in autophagy. Nat Struct Mol Biol. 20, 59-66 (2013)(PMID:23202584) Metlagel Z et al. Structural basis of ATG3 recognition by the autophagic ubiquitin-like protein ATG12. PNAS. 110, 18844-9 (2013)(PMID:24191030) Choi J et al. The parasitophorous vacuole membrane of Toxoplasma gondii is targeted for disruption by ubiquitin-like conjugation systems of autophagy. Immunity. 40, 924-35 (2014)(PMID:24931121) Yamashita SI et al. Mitochondrial division occurs concurrently with autophagosome formation but independently of Drp1 during mitophagy. J Cell Biol. 215, 649-665 (2016)(PMID:27903607) Allavena G et al. Suppressed translation and ULK1 degradation as potential mechanisms of autophagy limitation under prolonged starvation. Autophagy 12, 2085–2097 (2016)(PMID:27629431)
Product category	Research area Autophagy Cellular stress

The availability is based on the information in Japan at 00:05, Jul 30, 2021 in JST.
The special price is shown in red color.
Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
Abbreviations for applications：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
For applications and reactivity:
*: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
**: The use is reported from the licenser (Under evaluation or not tested by MBL).
For storage temparature: RT: room temparature
Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.