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HOME > Product search results > Code No. K0181-3 Anti-p53 (Human) mAb

Code No. K0181-3

Anti-p53 (Human) mAb

Price

¥30,000

Availability (in Japan)

10 or more

(In Japan at 17:00,
Aug 6, 2020 in JST)

Size

100 µg/100 µL

Data
  • Western Blotting

  • Immunoprecipitation

  • Immunohistochemistry

Clonality Monoclonal Clone DO-1
Isotype (Immunized Animal) Mouse IgG2a
Applications
WB
1 µg/mL  
IP
2 µg/200 µL of cell extract from 5x106 cells  
IH
2 µg/mL  
Immunogen (Antigen) human p53 N-terminus synthetic peptide (11-25 a.a.)
Reactivity [Gene ID]

Human[7157], Mouse(-), Rat(-)

Storage buffer 1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp. -20°C Conjugate Unlabeled Manufacturer MBL
Alternative names TP53
Background The tumor suppressor p53 controls numerous downstream targets that can result in variable outcomes, including apoptosis, transient growth arrest, and sustained growth arrest or senescence. p53 is induced on DNA damage to prevent gene amplification and preserve genetic stability. Induction of G1 arrest is dependent upon sequence-specific DNA binding and transcriptional activation of p53 target genes such as p21WAF-1/CIP-1. p21 is an inhibitor of cyclin dependent kinase (CDK) and blocks the activity of G1 cyclin-CDK complexes. In cells induction of p21 can block phosphorylation and inactivation of the retinoblastoma protein, thus contributing to G1 arrest. p53 functions to eliminate and inhibit the proliferation of abnormal cells, thereby preventing neoplastic development. Abrogation of the negative growth regulatory functions of p53 occurs in many, perhaps all, human tumors.
Related products D238-3 Anti-MORC3 mAb
CY-7050 CycLex® Phospho-p53 Ser46 ELISA Kit
CY-7051 CycLex® Phospho-p53 Ser392 ELISA Kit
Notes Reactivity of clone DO-1 to mouse or rat is not confirmed in our laboratory. However, it is reported that this clone reacts with mouse neural stem cells derived from ES cells (PMID: 24052948), and rat cardiomyocyte (PMID: 28783759).
Citations

Western Blotting

  1. Zhang X et al. Regulation of p21 by TWIST2 contributes to its tumor-suppressor function in human acute myeloid leukemia. Oncogene 34, 3000-10 (2015)(PMID:25088197)
  2. Liu W et al. Hepatitis B virus core protein inhibits Fas-mediated apoptosis of hepatoma cells via regulation of mFas/FasL and sFas expression. FASEB J. 29, 1113-23 (2015)(PMID:25466893)
  3. Sun R et al. Senescence as a novel mechanism involved in β-adrenergic receptor mediated cardiac hypertrophy. PLoS One 12, e0182668 (2017)(PMID:28783759)
  4. Schneider L et al. DNA damage in mammalian neural stem cells leads to astrocytic differentiation mediated by BMP2 signaling through JAK-STAT. Stem Cell Reports. 1, 123-138 (2013)(PMID:24052948)
  5. Wang Y et al. Deletion of INMAP postpones mitotic exit and induces apoptosis by disabling the formation of mitotic spindle. Biochem Biophys Res Commun. 518, 19-25 (2019)(PMID:31405563)

Immunoprecipitation

  1. Liu YZ et al. A panel of protein markers for the early detection of lung cancer with bronchial brushing specimens. Cancer Cytopathol. 122, 833-841 (2014)(PMID:25045014)
Product category
Cancer
Research area
Cancer
Apoptosis
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  • The availability is based on the information in Japan at 17:00, Aug 6, 2020 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications:
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
    DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.