Anti-IDH1 mAb

Price

¥37,400

Availability (in Japan)

10 or more

（In Japan at 17:00,
Apr 16, 2024 in JST）

Size

100 µL (1 mg/mL)

Citations

Data Sheet

SDS

Anti-IDH1 (Human) mAb (Clone: RcMab-1) reacts with wild type as well as mutated forms of IDH1 proteins such as R132H, R132S, R132L and R132G.

Data	Western Blotting
Clonality	Monoclonal	Clone	RcMab-1
Isotype (Immunized Animal)	Rat IgG2aκ
Applications	WB 0.5-1 μg/mL IC* reported. （PMID: 23750482） IH* reported. （PMID: 26006098）
Reactivity [Gene ID]	Human[3417], Hamster*(PMID: 23485467)
Storage buffer	1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp.	-20°C	Conjugate	Unlabeled	Manufacturer	MBL
Alternative names	isocitrate dehydrogenase 1 (NADP+), soluble
Background	Isocitrate dehydrogenase 1 (IDH1) mutations, which have been identified as early and frequent genetic alterations in astrocytomas, oligodendrogliomas, and oligoastrocytomas, as well as in secondary glioblastomas, might be the initiating event in these glioma subtypes. In contrast, primary glioblastomas, as well as other systemic cancers, rarely contain IDH1 mutations. The IDH1 mutations are remarkably specific to a single codon in the conserved and functionally important Arginine 132 residue (R132) in IDH1. Mutations in the analogous IDH2-R172 codon also occur at a lower rate in these cancers. IDH1/2 mutations were observed subsequently in 22% of acute myeloid leukemias. IDH1 mutations were found to result in the ability of the enzyme to catalyze the reduced NADP-dependent reduction of α-ketoglutarate to R(-)-2-hydroxyglutarate (2-HG). Reduction of α-ketoglutarate by 2-HG or mutant IDH1 results in a lower level of prolyl hydroxylases and promotes the accumulation of hypoxia-inducible factor (HIF) 1α. HIF-1α levels were greater in human gliomas harboring an IDH1 mutation than in tumors without the mutation. IDH1 appears to function as a tumor suppressor that, when mutationally inactivated, contributes to tumorigenesis in part through induction of the HIF-1 pathway.
Related products	D300-3 Anti-IDH1-R132S (Human) mAb D299-3 Anti-IDH1-R132H (Human) mAb D331-3 Anti-IDH1-R132G (Human) mAb D338-3 Anti-IDH2-R172W (Human) mAb D337-3 Anti-IDH2-R172M (Human) mAb D309-3 Anti-IDH1 mAb D330-3 Anti-IDH2 mAb D328-3 Anti-IDH2-R172K (Human) mAb D332-3 Anti-Mutated IDH1/2 (Human) mAb
Citations	Western Blotting Kaneko MK et al. Novel monoclonal antibodies GMab-r1 and LMab-1 specifically recognize IDH1-R132G and IDH1-R132L mutations. Monoclon Antib Immunodiagn Immunother. 32, 224-8 (2013)(PMID:23750482) Kato Y et al. A novel monoclonal antibody GMab-m1 specifically recognizes IDH1-R132G mutation. Biochem Biophys Res Commun. 432, 564-7 (2013)(PMID:23485467) Ogasawara S et al. Multi-specific monoclonal antibody MsMab-2 recognizes IDH1-R132L and IDH2-R172M mutations. Monoclon. Antib. Immunodiagn. Immunother., 32, 377-81 (2013)(PMID:24328739) Liu X et al. Isocitrate dehydrogenase 2 mutation is a frequent event in osteosarcoma detected by a multi-specific monoclonal antibody MsMab-1. Cancer Med. 2, 803-14 (2013)(PMID:24403254) Immunocytochemistry Kaneko MK et al. Novel monoclonal antibodies GMab-r1 and LMab-1 specifically recognize IDH1-R132G and IDH1-R132L mutations. Monoclon Antib Immunodiagn Immunother. 32, 224-8 (2013)(PMID:23750482) Immunohistochemistry Ikota H et al. Evaluation of IDH1 status in diffusely infiltrating gliomas by immunohistochemistry using anti-mutant and wild type IDH1 antibodies. Brain Tumor Pathol. (2015) In press. (PMID:26006098)
Product category	Research area Cancer Neuroscience

The availability is based on the information in Japan at 17:00, Apr 16, 2024 in JST.
The special price is shown in red color.
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Abbreviations for applications：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
For applications and reactivity:
*: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
**: The use is reported from the licenser (Under evaluation or not tested by MBL).
For storage temparature: RT: room temparature
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