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HOME > Product search results > Code No. D292-3 Anti-Mincle (Mouse) mAb

Code No. D292-3

Anti-Mincle (Mouse) mAb

Price

¥48,000

Availability (in Japan)

10 or more

(In Japan at 00:05,
Nov 17, 2019 in JST)

Size

100 µg/100 µL

Data
  • Immunoprecipitation

  • Flow Cytometry

Clonality Monoclonal Clone 4A9
Isotype (Immunized Animal) Rat IgG1 κ
Applications
WB
1-5 μg/mL  
IP
5-10 μg/200 μL cell extract from 1 x 106 cells  
FCM
5-10 μg/mL (final concentration)  
Function
1-10 μg/mL (final concentration)  (PMID: 18776906
Immunogen (Antigen) Mouse Mincle (full length) transfected RBL-2H3 cells
Reactivity [Gene ID]

Mouse[56619]

Storage buffer 1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp. -20°C Conjugate Unlabeled Manufacturer MBL
Alternative names Clec4e, C-type lectin domain family 4, member e, C86253, Clecsf9
Background Macrophage-inducible C-type lectin (Mincle, also called as Clec4e and Clecsf9), a type II transmembrane C-type lectin receptor, is expressed mainly in macrophages. Mincle is selectively associated with the Fc receptor common g-chain which contains immunoreceptor tyrosine-based activation motif (ITAM) and activates macrophages to produce inflammatory cytokines and chemokines. Mincle expressing cells are activated by spliceosome associated protein 130 (SAP130) which is a component of small nuclear ribonucleoprotein released from dead cells. Recently, Mincle is reported to recognize Mycobacterium tuberculosis as well as pathogenic fungus Malassezia species. Mincle is demonstrated to be an essential receptor for a mycobacterial glycolipid, trehalose-6,6’-dimycolate (TDM). The cytokine/chemokine production of macrophages which Malassezia or M.tuberculosis induces in Mincle-/- mice is significantly impaired. Mincle is considered to play a crucial role in immune responses to pathogens.
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Citations

Western Blotting

  1. Mohanraj M et al. The Mycobacterial Adjuvant Analogue TDB Attenuates Neuroinflammation via Mincle-Independent PLC-γ1/PKC/ERK Signaling and Microglial Polarization. Mol Neurobiol. (2018) In press.(PMID:29876879)
  2. Tan RZ et al. Curcumin relieved cisplatin-induced kidney inflammation through inhibiting Mincle-maintained M1 macrophage phenotype. Phytomedicine 52, 284-294 (2019)(PMID:30599909)

Flow Cytometry

  1. Miyake Y et al. C-type Lectin MCL Is an FcR?A-Coupled Receptor that Mediates the Adjuvanticity of Mycobacterial Cord Factor. Immunity. 38, 1050-62 (2013)(PMID:23602766)
  2. Toyonaga K et al. Characterization of the receptors for mycobacterial cord factor in Guinea pig. PLoS One 9, e88747 (2014)(PMID:24533147)
  3. Mohanraj M et al. The Mycobacterial Adjuvant Analogue TDB Attenuates Neuroinflammation via Mincle-Independent PLC-γ1/PKC/ERK Signaling and Microglial Polarization. Mol Neurobiol. (2018) In press.(PMID:29876879)
  4. Fites JS et al. An unappreciated role for neutrophil-DC hybrids in immunity to invasive fungal infections. PLoS Pathog. 14, e1007073 (2018)(PMID:29782541)

Function

  1. Yamasaki S et al. Mincle is an ITAM-coupled activating receptor that senses damaged cells. Nat Immunol. 9, 1179-88 (2008)(PMID:18776906)
Product category
Research area
Immunology
この製品に関するお問い合わせをする

  • The availability is based on the information in Japan at 00:05, Nov 17, 2019 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications:
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
    DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.