Anti-His-tag mAb-Agarose

Price

¥33,000

Availability (in Japan)

10 or more

（In Japan at 00:05,
Apr 19, 2024 in JST）

Size

Gel:200 µL (Slurry:400 µL)

Citations

Data Sheet

SDS

Data	Immunoprecipitation
Clonality	Monoclonal	Clone	OGHis
Isotype (Immunized Animal)	Mouse IgG2aκ
Applications	IP 20 µL of gel slurry/300 µL of cell extract from 1 x 10⁶ cells
Immunogen (Antigen)	6xHis tagged protein
Storage buffer	200 µg of antibody covalently coupled to 200 µL of agarose gel and provided as a 50% gel slurry suspended in PBS/0.1% ProClin 150 for a total volume of 400 µL.
Storage temp.	4°C	Conjugate	Agarose	Manufacturer	MBL
Background	The His-tag (6xHis-tag) is one of the most common tags used to facilitate the purification of recombinant proteins in Escherichia coli or other expression systems. This product is useful tool for immunoprecipitation of the His-tagged proteins, and it recognizes His-tags placed at N-terminal, C-terminal, and internal regions of the recombinant proteins.
Related products	PM032 Anti-His-tag pAb PM032-8 Anti-His-tag pAb-Agarose D291-3 Anti-His-tag mAb D291-6 Anti-His-tag mAb-Biotin D291-7 Anti-His-tag mAb-HRP-DirecT D291-10 Anti-His-tag mAb-Magnetic Agarose D291-A48 Anti-His-tag mAb-Alexa Fluor™ 488 D291-A59 Anti-His-tag mAb-Alexa Fluor™ 594 D291-A64 Anti-His-tag mAb-Alexa Fluor™ 647 M089-3 Anti-His-tag mAb M136-3 Anti-His-tag mAb D291-11 Anti-His-tag mAb-Magnetic Beads 3310 His tagged Protein PURIFICATION KIT 3310A His tagged Protein PURIFICATION KIT Trial Kit 3311 His tagged Protein PURIFICATION GEL 3310-205 His tag peptide
Citations	Immunoprecipitation Saito K et al. PODXL1 promotes metastasis of the pancreatic ductal adenocarcinoma by activating the C5aR/C5a axis from the tumor microenvironment. Neoplasia. 21, 1121-1132 (2019)(PMID:31759250)
Product category	Tools Epitope tags

The availability is based on the information in Japan at 00:05, Apr 19, 2024 in JST.
The special price is shown in red color.
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Abbreviations for applications：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
For applications and reactivity:
*: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
**: The use is reported from the licenser (Under evaluation or not tested by MBL).
For storage temparature: RT: room temparature
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