Anti-Flavocytochrome b₅₅₈ (Human) mAb-FITC

Price

¥33,000

Availability (in Japan)

10 or more

（In Japan at 00:05,
Apr 18, 2024 in JST）

Size

100 µL (500 µg/mL)

Citations

Data Sheet

SDS

Data	Flow Cytometry
Clonality	Monoclonal	Clone	7D5
Isotype (Immunized Animal)	Mouse IgG1
Applications	FCM 10 µg/mL (final concentration)
Immunogen (Antigen)	Human cytochrome b rich fraction
Reactivity [Gene ID]	Human[1535/1536]
Storage buffer	500 µg/mL in PBS/1% BSA/0.09% NaN₃
Storage temp.	4°C	Conjugate	FITC	Manufacturer	MBL
Alternative names	CYBA, CYBB, p22-PHOX, cytochrome b-245 light chain, p22phox, cytochrome b light chain, p22 phagocyte B-cytochrome, cytochrome
Background	The NADPH oxidase is a multicomponent enzyme that transfers electrons from NADPH to O₂ to generate superoxide (O₂^-), a key part of the phagocytic or neutrophilic respiratory burst response. Flavocytochrome b₅₅₈ is the catalytic component of the phagocyte NADPH oxidase. It is a transmembrane heterodimer composed of a large glycoprotein, gp91^phox (PHagocyte OXidase) and a smaller protein, p22^phox. Upon cell stimulation, flavocytochrome b₅₅₈ assembles with p67^phox, p47^phox, and the GTP-binding protein Rac and becomes activated to generate O₂^-. Mutations in gp91^phox, p22^phox, or other components of the NADPH oxidase can result in chronic granulomatous disease, which is associated with significant morbidity and mortality due to a predisposition to recurrent bacterial and fungal infections.
Related products	D162-3 Anti-Flavocytochrome b₅₅₈ (Human) mAb D162-5 Anti-Flavocytochrome b₅₅₈ (Human) mAb-PE
Citations	Flow Cytometry Bianchi M et al. Restoration of NET formation by gene therapy in CGD controls aspergillosis. Blood 114, 2619-22 (2009)(PMID:19541821) Hayee B et al. G6PC3 mutations are associated with a major defect of glycosylation: a novel mechanism for neutrophil dysfunction. Gycobiology 21, 914-24 (2011)(PMID:21385794) Uriarte SE et al. Granule exocytosis contributes to priming and activation of the human neutrophil respiratory burst. J Immunol. 391-400 (2011)(PMID:21642540) Makni-Maalej K et al. The TLR7/8 agonist CL097 primes N-formyl-methionyl-leucyl-phenylalanine-stimulated NADPH oxidase activation in human neutrophils: critical role of p47phox phosphorylation and the proline isomerase Pin1. J Immunol. 189, 4657-65 (2012)(PMID:23002436) De Chiara A et al. Characterization of cytosolic proliferating cell nuclear antigen (PCNA) in neutrophils: antiapoptotic role of the monomer. J Leukoc Biol. 94, 723-31 (2013)(PMID:23825390) Sürün D et al. High Efficiency Gene Correction in Hematopoietic Cells by Donor-Template-Free CRISPR/Cas9 Genome Editing. Mol Ther Nucleic Acids 10, 1-8 (2018)(PMID:29499925) Kiffin R et al. Anti-Leukemic Properties of Histamine in Monocytic Leukemia: The Role of NOX2. Front Oncol. 8, 218 (2018)(PMID:29967760) Kawai C et al. Monoclonal antibody 7D5 recognizes the R147 epitope on the gp91 phox , phagocyte flavocytochrome b 558 large subunit. Microbiol Immunol. 62, 269-280 (2018) (PMID:29573449)
Product category	Research area Signal transduction Cellular stress

The availability is based on the information in Japan at 00:05, Apr 18, 2024 in JST.
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Abbreviations for applications：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
For applications and reactivity:
*: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
**: The use is reported from the licenser (Under evaluation or not tested by MBL).
For storage temparature: RT: room temparature
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