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HOME > Product search results > Code No. D076-3 Anti-Phosphorylated Vimentin (Ser55) mAb

Code No.　D076-3

Anti-Phosphorylated Vimentin (Ser55) mAb

Price

¥52,800

Availability (in Japan)

10 or more

（In Japan at 00:05,
Apr 20, 2024 in JST）

Size

100 µL (1 mg/mL)

Data

  • Western Blotting

  • Immunocytochemistry
Clonality Monoclonal Clone 4A4
Isotype (Immunized Animal) Mouse IgG2b
Applications
WB
1-5 µg/mL  
IC
1 µg/mL  
IH*
reported.  （PMID: 15175243 / 15634780）
ELISA*
reported.  （PMID: 7983050）
Immunogen (Antigen) Synthetic MPV55 phophopeptide corresponding to Mouse phophorylated vimentin Ser55 (SLYSS-phosphoS55-PGGAYC-KLH)
Reactivity [Gene ID]

Human[7431], Mouse[22352], Rat[81818]
Storage buffer 1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp. -20°C Conjugate Unlabeled Manufacturer MBL
Alternative names Vim, vimentin
Background Vimentin is an intermediate filament protein distributed widely in the cytoplasm and is phosphorylated by several protein kinase in vitro. Ser55 residues on vimentin were reported to be one of the phosphorylation sites of vimentin at metaphase and were the phosphorylation sites for cdc2 kinase but not for cAMP-dependent protein kinase, protein kinase C, and Ca2+-calmodulin-dependent protein kinase II in vitro. Immunofluorescence and immunoelectron microscopy showed that vimentin Ser55 residues distributed in the entire cytoplasmic vimentin filament system are phosphorylated when the cells enter mitosis and de-phosphorylated in cytokinesis. The use of this antibody that specifically reacts with the phosphorylation site of vimentin Ser55 by cdc2 kinase enables estimation of a particular cdc2 kinase function.
Related products D093-3 Anti-Phosphorylated Vimentin (Ser71) mAb
D095-3 Anti-Phosphorylated Vimentin (Ser82) mAb
Citations

Western Blotting

  1. Tsujimura K et al. Visualization and function of vimentin phosphorylation by cdc2 kinase during mitosis. J Biol Chem. 269, 31097-106 (1994)(PMID:7983050)
  2. Fourest-Lieuvin A et al. Microtubule regulation in mitosis: tubulin phosphorylation by the cyclin-dependent kinase Cdk1. Mol Biol Cell. 17, 1041-50 (2006)(PMID:16371510)
  3. Tatsumi Y et al. Deregulation of Cdt1 induces chromosomal damage without rereplication and leads to chromosomal instability. J Cell Sci. 119, 3128-40 (2006)(PMID:16835273)
  4. Chang IA et al. Vimentin phosphorylation by Cdc2 in Schwann cell controls axon growth via β1-integrin activation. FASEB J. 26, 2401-13 (2012)(PMID:22371530)
  5. Yokoyama T et al. Identification of candidate molecular targets of the novel antineoplastic antimitotic NP-10. Sci Rep. 9, 16825 (2019)(PMID:31727981)

Immunocytochemistry

  1. Tsujimura K et al. Visualization and function of vimentin phosphorylation by cdc2 kinase during mitosis. J Biol Chem. 269, 31097-106 (1994)(PMID:7983050)
  2. Tsai J W et al. LIS1 RNA interference blocks neural stem cell division, morphogenesis, and motility at multiple stages. J Cell Biol. 170, 935-45 (2005)(PMID:16144905)
  3. Chang IA et al. Vimentin phosphorylation by Cdc2 in Schwann cell controls axon growth via β1-integrin activation. FASEB J. 26, 2401-13 (2012)(PMID:22371530)

Immunohistochemistry

  1. Miyata T et al. Asymmetric production of surface-dividing and non-surface-dividing cortical progenitor cells. Development. 131, 3133-45 (2004)(PMID:15175243)
  2. Englund C et al. Pax6, Tbr2, and Tbr1 are expressed sequentially by radial glia, intermediate progenitor cells, and postmitotic neurons in developing neocortex. J Neurosci. 25, 247-51 (2005)(PMID:15634788)
  3. Minobe S et al. Rac is involved in the interkinetic nuclear migration of cortical progenitor cells. Neurosci Res. 63, 294-301 (2009)(PMID:19367791)
  4. Shitamukai A et al. Oblique radial glial divisions in the developing mouse neocortex induce self-renewing progenitors outside the germinal zone that resemble primate outer subventricular zone progenitors. J Neurosci. 31, 3683-95 (2011)(PMID:21389223)
  5. Chang IA et al. Vimentin phosphorylation by Cdc2 in Schwann cell controls axon growth via β1-integrin activation. FASEB J. 26, 2401-13 (2012)(PMID:22371530)
  6. Martínez-Cerdeño V et al. N-Myc and GCN5 regulate significantly overlapping transcriptional programs in neural stem cells. PLoS One 7, e39456 (2012)(PMID:22745758)
  7. Malik S et al. Neurogenesis continues in the third trimester of pregnancy and is suppressed by premature birth. J Neurosci. 33, 411-23 (2013)(PMID:23303921)
  8. Martínez‐Cerdeño V et al. Evolutionary origin of Tbr2-expressing precursor cells and the subventricular zone in the developing cortex. J Comp Neurol. 524, 433-47 (2016)(PMID:26267763)
  9. Delgado RN, Lim DA. Embryonic Nkx2.1-expressing neural precursor cells contribute to the regional heterogeneity of adult V-SVZ neural stem cells. Dev Biol. 407, 265-74 (2015)(PMID:26387477)
  10. Wang L et al. Hedgehog signaling promotes basal progenitor expansion and the growth and folding of the neocortex. Nat Neurosci. 19, 888-96 (2016)(PMID:27214567)
  11. Garbriel E et al. Recent Zika Virus Isolates Induce Premature Differentiation of Neural Progenitors in Human Brain Organoids. Cell Stem Cell 20, 397-406.e5 (2017)(PMID:28132835)
  12. Bosshard M et al. Impaired oxidative stress response characterizes HUWE1-promoted X-linked intellectual disability. Sci Rep. 7,15050 (2017)(PMID:29118367)
  13. Zhou X et al. Cellular and molecular properties of neural progenitors in the developing mammalian hypothalamus. Nat Commun. 11, 4063 (2020)(PMID:32792525)
Product category
Research area
Signal transduction
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  • The availability is based on the information in Japan at 00:05, Apr 20, 2024 in JST.
  • The special price is shown in red color.
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  • Abbreviations for applications：
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
    DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.