キット・タンパク質・ベクターなど 製品詳細情報

Code No. CY-1182
CycLex® AMPK Kinase Assay Kit
価格(税別) ¥99,000
包装 96 assays
在庫 10以上
(2024/04/19 00:05時点)
キット構成品 Microplate, 10X Wash Buffer, Kinase Buffer, 20X ATP, 20X DTT, Anti-phospho-mouse IRS-1 S789 Monoclonal Antibody (AS-4C4), HRP conjugated Anti-mouse IgG, Substrate Reagent, Stop Solution
使用目的 This Kit is primarily designed to measure the activities of purified AMP-activated protein kinase (AMPK) or recombinant AMPK for the rapid and sensitive evaluation of activators or inhibitors.
保存温度 4°C
メーカー MBL
製品カテゴリー 分野:ドラッグディスカバリー シグナル伝達
データ

使用文献
  1. Wang Y et al.  Ghrelin inhibits insulin secretion through the AMPK_UCP2 pathway in β cells. FEBS Lett. 584, 1503-1508 (2010)(PMID:20206170)
  1. Li D et al. Adenosine Monophosphate Activated Protein Kinase Induces Cholesterol Efflux from Macrophage-Derived Foam Cells and Alleviates Atherosclerosis in Apolipoprotein E-Deficient Mice. J Biol Chem. 285, 33499-509 (2010)(PMID:20713354)
  1. Vingtdeux V et al. Novel synthetic small-molecule activators of AMPK as enhancers of autophagy and amyloid-β peptide degradation. FASEB J. 25, 219-31 (2011)(PMID:20852062)
  1. Tang YC et al. Identification of aneuploidy-selective antiproliferation compounds. Cell 144, 499-512 (2011)(PMID:21315436)
  1. Yeh CH et al. AMP-activated protein kinase activation during cardioplegia-induced hypoxia/reoxygenation injury attenuates cardiomyocytic apoptosis via reduction of endoplasmic reticulum stress. Mediators Inflamm. 2010, 130636 (2010)(PMID:21318153)
  1. Wang Z et al. Homocysteine suppresses lipolysis in adipocytes by activating the AMPK pathway. Am J Physiol Endocrinol Metab. 301, E703-12 (2011)(PMID:21750268)
  1. Yokoyama Y et al. AMP-activated protein kinase modulates the gene expression of aquaporin 9 via forkhead box a2. Arch Biochem Biophys. 515, 80-8 (2011)(PMID:21867676)
  1. Lee MS et al. Reduction of Body Weight by Dietary Garlic Is Associated with an Increase of Uncoupling Protein mRNA Expression and Activation of AMP-Activated Protein Kinase in Diet-Induced Obese Mice. J Nutr. 141, 1947-53 (2011)(PMID:21918057)
  1. Greco SJ et al. Leptin boosts cellular metabolism by activating AMPK and the sirtuins to reduce tau phosphorylation and β-amyloid in neurons. Biochem Biophys Res Commun. 414, 170-4 (2011)(PMID:21945934)
  1. Miyokawa-Gorin K et al. Induction of mitochondrial uncoupling enhances VEGF(120) but reduces MCP-1 release in mature 3T3-L1 adipocytes: Possible regulatory mechanism through endogenous ER stress and AMPK-related pathways. Biochem Biophys Res Commun. 419, 200-5 (2012)(PMID:22330806)
  1. Magnoni LJ et al. AMP-Activated Protein Kinase Plays an Important Evolutionary Conserved Role in the Regulation of Glucose Metabolism in Fish Skeletal Muscle Cells. PLoS One 7, e31219 (2012)(PMID:22359576)
  1. Bruckbauer A, Zemel MB. Synergistic effects of metformin, resveratrol, and hydroxymethylbutyrate on insulin sensitivity. Diabetes Metab Syndr Obes. 6, 93-102 (2013)(PMID:23430507)
  1. Figarola JL, Rahbar S. Small-molecule COH-SR4 inhibits adipocyte differentiation via AMPK activation. Int J Mol Med. 31, 1166-76 (2013)(PMID:23525347)
  1. Yamada S et al. AMP-activated protein kinase-mediated glucose transport as a novel target of tributyltin in human embryonic carcinoma cells. Metallomics. 5, 484-91 (2013)(PMID:23536109)
  1. Takikawa M et al. 10-Hydroxy-2-decenoic acid, a unique medium-chain fatty acid, activates 5'-AMP-activated protein kinase in L6 myotubes and mice. Mol Nutr Food Res. 57, 1794-802 (2013)(PMID:23754629)
  1. Seo JY, Cresswell P. Viperin regulates cellular lipid metabolism during human cytomegalovirus infection. PLoS Pathog. 9, e1003497 (2013)(PMID:23935494)
  1. Zhang X et al. Increased 4-hydroxynonenal formation contributes to obesity-related lipolytic activation in adipocytes. PLoS One. 8, e70663 (2013)(PMID:23940618)
  1. Hsu CY and Chuang YL. Changes in energy-regulated molecules in the trophocytes and fat cells of young and old worker honeybees (Apis mellifera). J Gerontol A Biol Sci Med Sci. 69, 955-64 (2014)(PMID:24149426)
  1. Liang Y et al. Constitutive activation of AMPK α1 in vascular endothelium promotes high-fat diet-induced fatty liver injury: role of COX-2 induction. Br J Pharmacol. 171, 498-508 (2014)(PMID:24372551)
  1. Kim JY et al. Anti-obesity efficacy of nanoemulsion oleoresin capsicum in obese rats fed a high-fat diet. Int J Nanomedicine. 9, 301-10 (2014)(PMID:24403834)
  1. Zadra G et al. A novel direct activator of AMPK inhibits prostate cancer growth by blocking lipogenesis. EMBO Mol Med. 6, 519-38 (2014)(PMID:24497570)
  1. Magnoni LJ et al. Fueling the engine: induction of AMP-activated protein kinase in trout skeletal muscle by swimming. J Exp Biol. 217, 1649-52 (2014)(PMID:24526729)
  1. Bruckbauer A, Zemel MB. Synergistic effects of polyphenols and methylxanthines with Leucine on AMPK/Sirtuin-mediated metabolism in muscle cells and adipocytes. PLoS One. 9, e89166 (2014)(PMID:24551237)
  1. Park H et al. Consensus scoring approach to identify the inhibitors of AMP-activated protein kinase α2 with virtual screening. J Chem Inf Model. 54, 2139-46 (2014)(PMID:24915156)
  1. Hsu CY, Hu TH. Energy-regulated molecules maintain young status in the trophocytes and fat cells of old queen honeybees. Biogerontology. 15, 389-400 (2014)(PMID:24973265)
  1. Kobashigawa LC et al. Metformin protects cardiomyocyte from doxorubicin induced cytotoxicity through an AMP-activated protein kinase dependent signaling pathway: an in vitro study. PLoS One. 9, e104888 (2014)(PMID:25127116)
  1. Lee MS et al. Effect of high hydrostatic pressure extract of fresh ginseng on adipogenesis in 3T3-L1 adipocytes. J Sci Food Agric. 10, 1002 (2014)(PMID:25323802)
  1. Noda K et al. Rho-kinase inhibition ameliorates metabolic disorders through activation of AMPK pathway in mice. PLoS One. 9, e110446 (2014)(PMID:25365359)
  1. Soga M et al. The di-peptide Trp-His activates AMP-activated protein kinase and enhances glucose uptake independently of insulin in L6 myotubes. FEBS Open Bio. 4, 898-904 (2014)(PMID:25383313)
  1. Sato S et al. Elevated levels of TWEAK in skeletal muscle promote visceral obesity, insulin resistance, and metabolic dysfunction. FASEB J. 29, 988-1002 (2015)(PMID:25466899)
  1. Zhou X et al. AMP-activated protein kinase is required for the anti-adipogenic effects of alpha-linolenic acid. Nutr Metab. 12,10 (2015)(PMID:25774202)
  1. Akhtar N et al. The pentacyclic triterpenoid, plectranthoic acid, a novel activator of AMPK induces apoptotic death in prostate cancer cells. Oncotarget 7, 3819-31 (2016)(PMID:26683363)
  1. Zhao C et al. Hypoxia promotes drug resistance in osteosarcoma cells via activating AMP-activated protein kinase (AMPK) signaling. J Bone Oncol. 5, 22-9 (2016)(PMID:26998423)
  1. Niopek K et al. A Hepatic GAbp-AMPK Axis Links Inflammatory Signaling to Systemic Vascular Damage. Cell Rep. 20, 1422-1434 (2017)(PMID:28793265)
  1. Jang J et al. A Berberine activates AMPK to suppress proteolytic processing, nuclear translocation and target DNA binding of SREBP-1c in 3T3-L1 adipocytes. Mol Med Rep. 15, 4139-4147 (2017)(PMID:28487951)
  1. Chen X et al. Novel direct AMPK activator suppresses non-small cell lung cancer through inhibition of lipid metabolism. Oncotarget 8, 96089-96102 (2017)(PMID:29221189)
  1. Rovira M et al. Exercise-Induced Hypertrophic and Oxidative Signaling Pathways and Myokine Expression in Fast Muscle of Adult Zebrafish. Front Physiol. 8, 1063 (2017)(PMID:29326600)
  1. Wang H et al. αdeficiency exacerbates long-term PM2.5 exposure-induced lung injury and cardiac dysfunction. Free Radic Biol Med. 121, 202-214 (2018)(PMID:29753072)
  1. Kang MJ et al. Polyacetylene From Dendropanax morbifera Alleviates Diet-Induced Obesity and Hepatic Steatosis by Activating AMPK Signaling Pathway. Front Pharmacol. 9, 537 (2018)(PMID:29875667)
  1. Mok SWF et al. A Method for Rapid Screening of Anilide-Containing AMPK Modulators Based on Computational Docking and Biological Validation. Front Pharmacol. 9, 710 (2018)(PMID:30018557)
  1. Kawasumi K et al. Changes in Plasma Metabolites Concentrations in Obese Dogs Supplemented With Anti-oxidant Compound. Front Nutr. 5, 74 (2018)(PMID:30246011)
  1. Lee MS, Kim Y. Effects of Isorhamnetin on Adipocyte Mitochondrial Biogenesis and AMPK Activation. Molecules 23, E1853 (2018)(PMID:30044453)
  1. Dai SN et al. Ginsenoside Rb1 Ameliorates Autophagy of Hypoxia Cardiomyocytes from Neonatal Rats via AMP-Activated Protein Kinase Pathway. Chin J Integr Med. (2018) In press.(PMID:30088211)
  1. Kim S et al. Ginger Extract Ameliorates Obesity and Inflammation via Regulating MicroRNA-21/132 Expression and AMPK Activation in White Adipose Tissue. Nutrients. 10, 1567 (2018)(PMID:30360535)
  1. Kim SY et al. Tartary Buckwheat Extract Attenuated the Obesity-Induced Inflammation and Increased Muscle PGC-1a/SIRT1 Expression in High Fat Diet-Induced Obese Rats. Nutrients. 11, pii: E654 (2019) (PMID:30889894)
  1. Chang E, Kim Y. Vitamin D Ameliorates Fat Accumulation with AMPK/SIRT1 Activity in C2C12 Skeletal Muscle Cells. Nutrients. 11, pii: E2806 (2019) (PMID:31744213)
  1. Si Y et al. Ethoxysanguinarine, a Novel Direct Activator of AMP-Activated Protein Kinase, Induces Autophagy and Exhibits Therapeutic Potential in Breast Cancer Cells. Front Pharmacol. 10, 1503 (2019)(PMID:31969821)
  1. Wu Y et al. Polyphyllin I activates AMPK to suppress the growth of non-small-cell lung cancer via induction of autophagy. Arch Biochem Biophys. 687, 108285 (2020)(PMID:32074500)
  1. Lee MS, Kim Y. Mulberry Fruit Extract Ameliorates Adipogenesis via Increasing AMPK Activity and Downregulating MicroRNA-21/143 in 3T3-L1 Adipocytes. J Med Food. 23, 266-272 (2020)(PMID:32191574)
  1. Ren G et al. Berberine Improves Glucose and Lipid Metabolism in HepG2 Cells Through AMPKα1 Activation. Front Pharmacol. 11, 647 (2020)(PMID:32457629)
  1. Kondo K et al. Pre-treatment with D942, a furancarboxylic acid derivative, increases desiccation tolerance in an anhydrobiotic tardigrade Hypsibius exemplaris. FEBS Open Bio. 10, 1774-1781 (2020)(PMID:32623826)

※在庫につきましては2024年04月19日 00時05分時点における在庫数を表示してあります。
※価格の赤色表示につきましては、キャンペーン価格の表示となっております。
※このサイトからは直接注文はできません。ご注意ください。
※保存温度の表記について: RT: 室温
※本サイトの掲載製品は、品質改良のため予告なく仕様などの変更、または販売中止となる場合がございます。あらかじめご了承ください。

MBLCopyright (C)2015 Medical & Biological Laboratories Co., Ltd. All Rights Reserve