キット・タンパク質・ベクターなど 製品詳細情報

Code No. CY-1161
CycLex® Cyclic GMP dependent protein kinase (cGK) Assay Kit
価格(税別) ¥99,000
包装 96 assays
在庫 1-9
（2024/04/19 17:00時点）
キット構成品 Microplate (coated with recombinant G-kinase substrate), 10×Wash Buffer, Kinase Buffer, 20×ATP, HRP conjugated Detection Antibody, Substrate Reagent, Stop Solution
使用目的 This Kit is primarily designed to measure the activities of purified the cGK family of kinases for the rapid and sensitive evaluation of inhibitors or activators.
保存温度 4°C
メーカー MBL
関連製品 CY-E1161-1 cGK Positive Control (Catalytic Domain)
CY-E1161-2 cGK Positive Control (Full length)
製品カテゴリー 分野:ドラッグディスカバリー シグナル伝達
使用文献
  1. Nie HG et al. 8-pCPT-cGMP stimulates alpha beta gamma ENaC activity in oocytes as an external ligand requiring specific nucleotide moieties. Am J Physiol Renal Physiol. 298, F323-34 (2010)(PMID:20007351)
  1. Bogaard HJ et al. Adrenergic Receptor Blockade Reverses Right Heart Remodeling and Dysfunction in Pulmonary Hypertensive Rats. Am J Respir Crit Care Med. 182, 652-60 (2010)(PMID:20508210)
  1. Haider HKh et al. Phosphodiestrase inhibition with tadalafil provides longer and sustained protection of stem cells. Am J Physiol Heart Circ Physiol. 299, H1395-404 (2010)(PMID:20833962)
  1. Larocca TJ et al. beta2-Adrenergic Receptor Signaling in the Cardiac Myocyte is Modulated by Interactions with CXCR4. J Cardiovasc Pharmacol. 56, 548-59 (2010)(PMID:20729750)
  1. Koka S et al. Long-Acting Phosphodiesterase-5 Inhibitor Tadalafil Attenuates Doxorubicin-Induced Cardiomyopathy without Interfering with Chemotherapeutic Effect. J Pharmacol Exp Ther. 334, 1023-30 (2010)(PMID:20543097)
  1. Chau VQ et al. Mitigation of the Progression of Heart Failure with Sildenafil Involves Inhibition of RhoA/Rho-Kinase Pathway. Am J Physiol Heart Circ Physiol. 300, H2272-9 (2011)(PMID:21398594)
  1. Gebska MA et al. Phosphodiesterase-5A (PDE5A) is localized to the endothelial caveolae and modulates NOS3 activity. Cardiovasc Res. 90, 353-63 (2011)(PMID:21421555)
  1. Aggarwal S et al. Black; Purification and functional analysis of protein kinase G-1α using a bacterial expression system. Protein Expr Purif. 79, 271-6 (2011)(PMID:21600289)
  1. Hu LF et al. Hydrogen sulfide regulates Na+/H+ exchanger activity via stimulation of Phosphoinositide 3-kinase/Akt and protein kinase G pathways. J Pharmacol Exp Ther. 339, 726-35 (2011)(PMID:21865440)
  1. Aggarwal S et al. Attenuated vasodilatation in lambs with endogenous and exogenous activation of cGMP signaling: role of protein kinase G nitration. J Cell Physiol. 226, 3104-13 (2011)(PMID:21351102)
  1. Chan S et al. Amelioration of central cardiovascular regulatory dysfunction by tropomyocin receptor kinase B in mevinphos intoxication model of brain stem death. Br J Pharmacol.164, 2015-28 (2011)(PMID:21615729)
  1. Hoke NN et al.  Preconditioning by phosphodiesterase-5 inhibition improves therapeutic efficacy of adipose-derived stem cells following myocardial infarction in mice. Stem Cells. 30, 326-35 (2012)(PMID:22102597)
  1. Salloum FN et al. Cinaciguat - a novel activator of soluble guanylate cyclase, protects against ischemia/reperfusion injury: Role of hydrogen sulfide. Am J Physiol Heart Circ Physiol. 302, H1347-54 (2012)(PMID:22268103)
  1. Hesabi B et al. Heterogeneous nuclear ribonucleoprotein A1 is a novel cellular target of atrial natriuretic peptide signaling in renal epithelial cells. Cell Signal. 24, 1100-8 (2012)(PMID:22285803)
  1. Nagasu H et al. Endothelial dysfunction promotes the transition from compensatory renal hypertrophy to kidney injury after unilateral nephrectomy in mice. Am J Physiol Renal Physiol. 302, F1402-8 (2012)(PMID:22378818)
  1. Seya K et al. Cytosolic Ca2+-induced apoptosis in rat cardiomyocytes via mitochondrial NO-cGMP-protein kinase G pathway. J Pharmacol Exp Ther. 344, 77-84 (2013)(PMID:23104881)
  1. Zhou K et al. 17β-estradiol induces vasorelaxation by stimulating endothelial hydrogen sulfide release. Mol Hum Reprod. 19, 169-76 (2013)(PMID:23041593)
  1. Jaba IM et al. NO triggers RGS4 degradation to coordinate angiogenesis and cardiomyocyte growth. J Clin Invest. 123, 1718-31 (2013)(PMID:23454748)
  1. Wang L et al. Protein kinase G1 α overexpression increases stem cell survival and cardiac function after myocardial infarction. PLoS One. 8, e60087 (2013)(PMID:23536905)
  1. Tassone EJ et al. Angiotensin (1-7) counteracts the negative effect of angiotensin II on insulin signalling in HUVECs. Cardiovasc Res. 99, 129-36 (2013)(PMID:23524303)
  1. Keating JA et al. Mosquito protein kinase G phosphorylates flavivirus NS5 and alters flight behavior in Aedes aegypti and Anopheles gambiae. Vector Borne Zoonotic Dis. 13, 590-600 (2013)(PMID:23930976)
  1. Jin Z et al. Beneficial effects of tadalafil on left ventricular dysfunction in doxorubicin-induced cardiomyopathy. J Cardiol. 62, 110-6 (2013)(PMID:23731918)
  1. Tarès S et al. Environment exploration and colonization behavior of the pea aphid associated with the expression of the foraging gene. PLoS One. 8, e65104 (2013)(PMID:23734236)
  1. Ranek MJ et al. Protein kinase g positively regulates proteasome-mediated degradation of misfolded proteins. Circulation. 128, 365-76 (2013)(PMID:23770744)
  1. Andersen A et al. Right ventricular hypertrophy and failure abolish cardioprotection by ischaemic pre-conditioning. Eur J Heart Fail. 15, 1208-14 (2013)(PMID:23818649)
  1. Bivalacqua TJ et al. Sildenafil citrate-restored eNOS and PDE5 regulation in sickle cell mouse penis prevents priapism via control of oxidative/nitrosative stress. PLoS One. 8, e68028 (2013)(PMID:23844149)
  1. Keating JA et al. West Nile virus methyltransferase domain interacts with protein kinase G. Virol J. 10, 242 (2013)(PMID:23876037)
  1. Xu J et al. cGMP accumulation causes photoreceptor degeneration in CNG channel deficiency: evidence of cGMP cytotoxicity independently of enhanced CNG channel function. J Neurosci. 33, 14939-48 (2013)(PMID:24027293)
  1. Sagredo A et al. Ovariectomy increases the participation of hyperpolarizing mechanisms in the relaxation of rat aorta. PLoS One. 8, e73474 (2013)(PMID:24058477)
  1. Aggarwal S et al. Nitration of tyrosine 247 inhibits protein kinase G-1α activity by attenuating cyclic guanosine monophosphate binding. J Biol Chem. 289, 7948-61 (2014)(PMID:24469460)
  1. Karakhanova S et al. Interlude of cGMP and cGMP/protein kinase G type 1 in pancreatic adenocarcinoma cells. Pancreas. 43, 784-94 (2014)(PMID:24826884)
  1. Sasaki H et al. PDE5 inhibitor efficacy is estrogen dependent in female heart disease. J Clin Invest. 124, 2464-71 (2014)(PMID:24837433)
  1. da Silva Lima V et al. Uroguanylin inhibits H-ATPase activity and surface expression in renal distal tubules by a PKG-dependent pathway. Am J Physiol Cell Physiol. 307, C532-41 (2014)(PMID:25031022)
  1. Lee KH et al. Cardioprotective effects of PKG activation by soluble GC activator, BAY 60-2770, in ischemia-reperfusion-injured rat hearts. PLoS One 12, e0180207 (2017)(PMID:28671970)
  1. Song Q et al. Sildenafil improves blood perfusion in steroid-induced avascular necrosis of femoral head in rabbits via a protein kinase G-dependent mechanism. Acta Orthop Traumatol Turc. 51, 398-403 (2017)(PMID:28774681)
  1. Zhang RG et al. Regulation of Intracellular Calcium by Carbon Monoxide in Human Bronchial Epithelial Cells. Cell Physiol Biochem. 42, 2377-2390 (2017)(PMID:28957808)
  1. Cortese-Krott MM et al. Identification of a soluble guanylate cyclase in RBCs: preserved activity in patients with coronary artery disease. Redox Biol. 14, 328-337 (2018)(PMID:29024896)
  1. Zhang Q et al. PPARγ Antagonizes Hypoxia-Induced Activation of Hepatic Stellate Cell through Cross Mediating PI3K/AKT and cGMP/PKG Signaling. PPAR Res. 2018, 6970407 (2018)(PMID:29686697)
  1. Caracuel L et al. Acute-on-chronic liver disease enhances phenylephrine-induced endothelial nitric oxide release in rat mesenteric resistance arteries through enhanced PKA, PI3K/AKT and cGMP signalling pathways. Sci Rep. 9, 6993 (2019)(PMID:31061522)
  1. Caracuel L ＜em>et al. Acute-on-chronic liver disease enhances phenylephrine-induced endothelial nitric oxide release in rat mesenteric resistance arteries through enhanced PKA, PI3K/AKT and cGMP signalling pathways. Sci Rep. 9, 6993 (2019)(PMID:31061522)
  1. Waddingham MT et al. Diastolic dysfunction is initiated by cardiomyocyte impairment ahead of endothelial dysfunction due to increased oxidative stress and inflammation in an experimental prediabetes model. J Mol Cell Cardiol. 137, 119-131 (2019)(PMID:31669609)
  1. Wen JJ et al. Sildenafil Recovers Burn-Induced Cardiomyopathy. Cells. 9, 1393 (2020)(PMID:32503314)
  1. Das S et al. Depletion of cyclic-GMP levels and inhibition of cGMP-dependent protein kinase activate p21Cip1/p27Kip1 pathways and lead to renal fibrosis and dysfunction. FASEB J. 34, 11925-11943 (2020)(PMID:32686172)
  1. Ranek MJ et al. CHIP phosphorylation by protein kinase G enhances protein quality control and attenuates cardiac ischemic injury. Nat Commun. 11, 5237 (2020)(PMID:33082318)

※在庫につきましては2024年04月19日 17時00分時点における在庫数を表示してあります。
※価格の赤色表示につきましては、キャンペーン価格の表示となっております。
※このサイトからは直接注文はできません。ご注意ください。
※保存温度の表記について： RT: 室温
※本サイトの掲載製品は、品質改良のため予告なく仕様などの変更、または販売中止となる場合がございます。あらかじめご了承ください。

MBLCopyright (C)2015 Medical ＆ Biological Laboratories Co., Ltd. All Rights Reserve