抗体 製品詳細情報

Code No. RN019P プリントプリント
Anti-HNRNPK pAb
価格(税別) ¥60,000
包装 200 µg/200 µL
在庫 10以上
(2019/11/22 00:05時点)
クローナリティー Polyclonal
クローン Polyclonal
アイソタイプ
(免疫動物)
Rabbit Ig (aff.)
使用法 WB 1 µg/mL  
IP 5 µg/500 µL of cell extract from 5 x 106 cells  
IC* reported.  (PMID: 24990929 / 26990993)
RIP 15 µg/500 µL of cell extract from 1.5 x 107 cells  
Other(eCLIP) reported.  (PMID: 27018577)
免疫原(抗原) KLH conjugated synthetic peptide, corresponding to C-terminus of human HNRNPK
交差性 [Gene ID]

Human[3190], Mouse[15387], Rat[117282]

性状 1 mg/mL in PBS/50% glycerol, pH 7.2
保存温度 -20°C
標識 Unlabeled
メーカー MBL
別称 heterogeneous nuclear ribonucleoprotein K, RP11-575L7.1, CSBP, HNRPK, TUNP
背景 The heterogeneous nuclear ribonucleoprotein K (hnRNP K), is an RNA-binding protein containing 3 KH RNA-binding domains. It binds to both RNA and DNA and is a component of the hnRNP complex. hnRNP K is associated with pre-mRNA and appears to influence the processing of pre-mRNAs and metabolism of mRNA. hnRNP K is predominantly localized at the nucleus and shuttles between the nucleus and the cytoplasm. Therefore, hnRNP K is thought to be involved in the transport of mRNA. Phosphorylation of hnRNP K leads to its cytoplasmic accumulation and is important for cell migration and metastasis. In the cytoplasm, hnRNP K functions as a translational regulator of specific mRNAs such as c-myc mRNA, 16L2 mRNA, and r15-LOX mRNA. Further, it specifically associates with c-src kinase, leading to c-src activation and hnRNP K phosphorylation, which affect the binding of hnRNP K to RNA.
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RN1005 RIP-Assay Kit for microRNA
RN052PW Anti-HNRNPC pAb
RN060PW Anti-HNRNPD (AUF1) pAb
RN011P Anti-PTBP1 (Human) pAb
RN024P Anti-PCBP1 pAb
RN025P Anti-PCBP2 pAb
RN114PW Anti-HNRNPA1 pAb
製品カテゴリー 分野:RNA-RNPネットワーク
ブランド:使用文献・データ募集プレゼント対象製品 RiboCluster Profiler™
データ



使用文献

Western Blotting

  1. Folci A et al. Loss of hnRNP K impairs synaptic plasticity in hippocampal neurons. J Neurosci. 34, 9088-95 (2014)(PMID:24990929)
  2. Chung BM et al. Regulation of C-X-C chemokine gene expression by keratin 17 and hnRNP K in skin tumor keratinocytes. J Cell Biol. 208, 613-27 (2015)(PMID:25713416)

Immunoprecipitation

  1. Chung BM et al. Regulation of C-X-C chemokine gene expression by keratin 17 and hnRNP K in skin tumor keratinocytes. J Cell Biol. 208, 613-27 (2015)(PMID:25713416)

Immunocytochemistry

  1. Folci A et al. Loss of hnRNP K impairs synaptic plasticity in hippocampal neurons. J Neurosci. 34, 9088-95 (2014)(PMID:24990929)
  2. Chung BM et al. Regulation of C-X-C chemokine gene expression by keratin 17 and hnRNP K in skin tumor keratinocytes. J Cell Biol. 208, 613-27 (2015)(PMID:25713416)
  3. Sundararaman B et al. Resources for the Comprehensive Discovery of Functional RNA Elements. Mol Cell. 61, 903-13 (2016)(PMID:26990993)

RNP Immunoprecipitation

  1. Chung BM et al. Regulation of C-X-C chemokine gene expression by keratin 17 and hnRNP K in skin tumor keratinocytes. J Cell Biol. 208, 613-27 (2015)(PMID:25713416)
  2. Lubelsky Y, Ulitsky I. Sequences enriched in Alu repeats drive nuclear localization of long RNAs in human cells. Nature 555, 107-111 (2018)(PMID:29466324)

Other(eCLIP)

  1. Van Nostrand EL et al. Robust transcriptome-wide discovery of RNA-binding protein binding sites with enhanced CLIP (eCLIP). Nat Methods. 13, 508-14 (2016)(PMID:27018577)

※在庫につきましては2019年11月22日 00時05分時点における在庫数を表示してあります。
※価格の赤色表示につきましては、キャンペーン価格の表示となっております。
※このサイトからは直接注文はできません。ご注意ください。
※使用法の表記について:
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation,
FCM: Flow Cytometry NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation,
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation,
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization

ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
※使用法・交差性の表記について:
*: 論文で報告されております(MBLでは未確認)。詳しくはデータシートをご覧ください。
**: 導入元からの情報です(MBLでは評価中もしくは未確認)。
※保存温度の表記について: RT: 室温
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