抗体 製品詳細情報

Code No. RN007P
Anti-IGF2BP1 (IMP1) pAb
価格(税別) ¥66,000
包装 200 µL (1 mg/mL)
在庫 10以上
（2024/04/19 17:00時点）
クローナリティー Polyclonal
クローン Polyclonal
アイソタイプ
(免疫動物)		 Rabbit Ig (aff.)
使用法 WB　1:1,000  
IP　5 µg/250 µL of cell extract from 2.5 x 106 cells  
IC　Can be used.  
RIP　15 µg/500 µL of cell extract from 1.2 x 107 cells  
Other(eCLIP)　reported.  （PMID: 27018577 / 27068461）
Other(iCLIP)　reported.  （PMID: 29245011）
免疫原(抗原) KLH-conjugated synthetic peptide KQQHQKGQSNQAQARRK（561-577 aa)
交差性 [Gene ID]

Human[10642], Mouse[140486](PMID: 22215810), Rat(-), Hamster(-)
性状 1 mg/mL in PBS/50% glycerol, pH 7.2
保存温度 -20°C
標識 Unlabeled
メーカー MBL
別称 insulin-like growth factor 2 mRNA binding protein 1, ZBP1, CRDBP, IMP-1, CRD-BP, VICKZ1/ Zbp1, Crdbp
背景 The localization of IGF2 mRNA-binding protein 1 (IMP1) is associated with motility and the major functions of IMP1 are carried out by the phylogenetically conserved KH domains. IMP1 can affect stability, localization, and translation of its target RNAs. Binding of IMP1 to the leader 3 mRNA in the 5’-UTR of IGF2 inhibits translation from a leader 3 reporter mRNA. Aberrant IMP1 expression may interfere with c-myc regulation. Compared with copy number gains at 17q21 (IMP1 locus), gains at 8q24 (c-myc locus) were observed more frequently in tumors. Furthermore, IMP1 expression was frequently detected in tumors, implying mechanism of activation other than gene amplification.
関連製品 RN1001 RIP-Assay Kit
RN1005 RIP-Assay Kit for microRNA
RN001M Anti-IGF2BP1 (IMP1) mAb
RN008P Anti-IGF2BP2 (IMP2) pAb
RN009P Anti-IGF2BP3 (IMP3) pAb
RN048PW Anti-G3BP1 pAb
製品カテゴリー 分野:RNA-RNPネットワーク 神経科学
ブランド:RiboCluster Profiler™
データ



使用文献

Western Blotting

  1. King DT et al. Assessing specific oligonucleotides and small molecule antibiotics for the ability to Inhibit the CRD-BP-CD44 RNA interaction. PLoS One 9, e91585 (2014)(PMID:24622399)
  2. Conway AE et al. Enhanced CLIP uncovers IMP Protein-RNA targets in human pluripotent stem cells Important for cell adhesion and survival. Cell Rep. 15, 666-79 (2016)(PMID:27068461)
  3. Donlin-Asp PG et al. The Survival of Motor Neuron Protein Acts as a Molecular Chaperone for mRNP Assembly. Cell Rep. 18, 1660-1673 (2017)(PMID:28199839)
  4. JnBaptiste CK et al. Dicer loss and recovery induce an oncogenic switch driven by transcriptional activation of the oncofetal Imp1-3 family. Genes Dev. 31, 674-687 (2017)(PMID:28446596)
  5. Hosono Y et al. Oncogenic Role of THOR, a Conserved Cancer/Testis Long Non-coding RNA. Cell 171, 1559-1572 (2017)(PMID:29245011)

Immunoprecipitation

  1. Stoskus M et al. ETV6/RUNX1 transcript is a target of RNA-binding protein IGF2BP1 in t(12;21)(p13;q22)-positive acute lymphoblastic leukemia. Blood Cells Mol Dis. 57, 30-4 (2016)(PMID:26852652)
  2. Conway AE et al. Enhanced CLIP uncovers IMP Protein-RNA targets in human pluripotent stem cells Important for cell adhesion and survival. Cell Rep. 15, 666-79 (2016)(PMID:27068461)
  3. Hosono Y et al. Oncogenic Role of THOR, a Conserved Cancer/Testis Long Non-coding RNA. Cell 171, 1559-1572 (2017)(PMID:29245011)

Immunocytochemistry

  1. Sundararaman B et al. Resources for the Comprehensive Discovery of Functional RNA Elements. Mol Cell. 61, 903-13 (2016)(PMID:26990993)
  2. Donlin-Asp PG et al. The Survival of Motor Neuron Protein Acts as a Molecular Chaperone for mRNP Assembly. Cell Rep. 18, 1660-1673 (2017)(PMID:28199839)
  3. Markmiller S et al. Context-Dependent and Disease-Specific Diversity in Protein Interactions within Stress Granules. Cell 172, 590-604.e13 (2018)(PMID:29373831)

RNP Immunoprecipitation

  1. Patel VL et al. Spatial arrangement of an RNA zipcode identifies mRNAs under post-transcriptional control. Genes Dev. 26, 43-53 (2012)(PMID:22215810)
  2. King DT et al. Assessing specific oligonucleotides and small molecule antibiotics for the ability to Inhibit the CRD-BP-CD44 RNA interaction. PLoS One 9, e91585 (2014)(PMID:24622399)
  3. Conway AE et al. Enhanced CLIP uncovers IMP Protein-RNA targets in human pluripotent stem cells Important for cell adhesion and survival. Cell Rep. 15, 666-79 (2016)(PMID:27068461)
  4. Donlin-Asp PG et al. The Survival of Motor Neuron Protein Acts as a Molecular Chaperone for mRNP Assembly. Cell Rep. 18, 1660-1673 (2017)(PMID:28199839)
  5. de Vasconcellos JF et al. IGF2BP1 overexpression causes fetal-like hemoglobin expression patterns in cultured human adult erythroblasts. PNAS. 114, E5664-E5672 (2017)(PMID:28652347)
  6. Hosono Y et al. Oncogenic Role of THOR, a Conserved Cancer/Testis Long Non-coding RNA. Cell 171, 1559-1572 (2017)(PMID:29245011)

Other(eCLIP)

  1. Conway AE et al. Enhanced CLIP uncovers IMP Protein-RNA targets in human pluripotent stem cells Important for cell adhesion and survival. Cell Rep. 15, 666-79 (2016)(PMID:27068461)
  2. Van Nostrand EL et al. Robust transcriptome-wide discovery of RNA-binding protein binding sites with enhanced CLIP (eCLIP). Nat Methods. 13, 508-14 (2016)(PMID:27018577)

Other(iCLIP)

  1. Hosono Y et al. Oncogenic Role of THOR, a Conserved Cancer/Testis Long Non-coding RNA. Cell 171, 1559-1572 (2017)(PMID:29245011)

※在庫につきましては2024年04月19日 17時00分時点における在庫数を表示してあります。
※価格の赤色表示につきましては、キャンペーン価格の表示となっております。
※このサイトからは直接注文はできません。ご注意ください。
※使用法の表記について：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation,
FCM: Flow Cytometry NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation,
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation,
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
※使用法・交差性の表記について：
*: 論文で報告されております（MBLでは未確認）。詳しくはデータシートをご覧ください。
**: 導入元からの情報です（MBLでは評価中もしくは未確認）。
※保存温度の表記について： RT: 室温
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