抗体 製品詳細情報

Code No. PM021
Anti-S-tag pAb
価格(税別) ¥35,200
包装 100 µL
在庫 1-9
(2024/04/20 00:05時点)
クローナリティー Polyclonal
クローン Polyclonal
アイソタイプ
(免疫動物)
Rabbit Ig (aff.)
使用法 WB 1:1,000  
IP 5 µL/sample  
免疫原(抗原) Carrier protein (CP) conjugated synthetic peptide (CP-KETAAAKFERQHIDSC)
性状 PBS/50% glycerol, pH 7.2
保存温度 -20°C
標識 Unlabeled
メーカー MBL
背景 Epitope tagging is a powerful and versatile strategy for detecting and purifying proteins expressed by cloned genes. Short sequences encoding the epitope tag are cloned in-frame with target DNA to
produce fusion proteins containing the epitope tag peptide. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Anti-epitope tag antibodies can serve as universal purification or detection reagents for any tag-containing protein. S-tag is an epitope tag composed of a 15 residues peptide, KETAAAKFERQHMDS, derived from the pancreatic ribonuclease A. S-tag can combine with S-protein to form functional RNase S, allowing detection and quantification of S-tag fusion proteins by enzymatic assays. However, antibody against the S-tag sequence provides a more convenient and flexible method to easily identify, detect, or purify S-tag containing fusion proteins.
製品カテゴリー ツール:エピトープタグ
データ

使用文献

Western Blotting

  1. Ogawa D et al. Molecular characterization of the rice protein RSS1 required for meristematic activity under stressful conditions. Plant Physiol. Biochem. 61, 54-60 (2012)(PMID:23041461)
  2. O'Rourke TW et al. Amyloid-like assembly of the low complexity domain of yeast Nab3. Prion 9, 34-47 (2015)(PMID:25611193)
  3. O'Rourke TW, Reines D. Determinants of Amyloid Formation for the Yeast Termination Factor Nab3. PLoS One 11, e0150865 (2016)(PMID:26954508)
  4. Zhao J et al. GtfA Interacting with GtfB is Required for PsrP Glycosylation in Streptococcus pneumoniae. Jundishapur J Microbiol. 11, e68982 (2018)
  5. Kadooka C et al. Mitochondrial citrate transporters CtpA and YhmA are required for extracellular citric acid accumulation and contribute to cytosolic acetyl-CoA generation in Aspergillus luchuensis mut. kawachii. Appl Environ Microbiol. (2019) In press.(PMID:30737343)
  6. Hosokawa K et al. The Lysosome Pathway Degrades CD81 on the Cell Surface by Poly-ubiquitination and Clathrin-Mediated Endocytosis. Biol Pharm Bull. 43, 540-545 (2020)(PMID:31902824)

Immunoprecipitation

  1. Hosokawa K et al. The Lysosome Pathway Degrades CD81 on the Cell Surface by Poly-ubiquitination and Clathrin-Mediated Endocytosis. Biol Pharm Bull. 43, 540-545 (2020)(PMID:31902824)

※在庫につきましては2024年04月20日 00時05分時点における在庫数を表示してあります。
※価格の赤色表示につきましては、キャンペーン価格の表示となっております。
※このサイトからは直接注文はできません。ご注意ください。
※使用法の表記について:
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation,
FCM: Flow Cytometry NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation,
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation,
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization

ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
※使用法・交差性の表記について:
*: 論文で報告されております(MBLでは未確認)。詳しくはデータシートをご覧ください。
**: 導入元からの情報です(MBLでは評価中もしくは未確認)。
※保存温度の表記について: RT: 室温
※本サイトの掲載製品は、品質改良のため予告なく仕様などの変更、または販売中止となる場合がございます。あらかじめご了承ください。

MBLCopyright (C)2015 Medical & Biological Laboratories Co., Ltd. All Rights Reserve