抗体 製品詳細情報

Code No. PM005
Anti-RFP pAb
The antibody reacts with DsRed, mRFP1, mCherry, mOrange, mPlum, mStrawberry and tdTomato
価格(税別) ¥52,800
包装 100 µL
在庫 10以上
(2024/04/20 00:05時点)
クローナリティー Polyclonal
クローン Polyclonal
アイソタイプ
(免疫動物)
Rabbit IgG
使用法 WB 1:1,000  
IC 1:500  
IH* reported.  (PMID: 21331036 / 20534674 / 20008127)
Other(Immuno-EM) reported.  (PMID: 29481606)
免疫原(抗原) RFP
交差性 [Gene ID]

Other

性状 PBS/50% glycerol, pH 7.2
保存温度 -20°C
標識 Unlabeled
メーカー MBL
別称 Red fluorescent protein
背景 Expression vectors containing a protein and a tag protein are commonly used. Red Fluorescent Protein (RFP) fusion protein expression system is preferably used in various laboratories because of an easy monitoring of fusion proteins. This specific antibody for RFP is useful tool for monitoring of the fusion protein expression.
関連製品 PM005-7 Anti-RFP pAb-HRP-DirecT
M155-3 Anti-RFP mAb
M165-3 Anti-RFP mAb
M165-8 Anti-RFP mAb-Agarose
M204-7 Anti-RFP mAb HRP-DirecT
M208-3 Anti-RFP mAb Cocktail
M204-3 Anti-RFP mAb
M165-11 Anti-RFP mAb-Magnetic Beads
PM073 Anti-Renilla GFP pAb
製品カテゴリー ツール:エピトープタグ 蛍光タンパク
データ


使用文献

Western Blotting

  1. Ishida Y et al. Autophagic elimination of misfolded procollagen aggregates in the endoplasmic reticulum as a means of cell protection. Mol Biol Cell. 20, 2744-54 (2009)(PMID:19357194)
  2. Yamamoto H et al. Functional cross-talk between Rab14 and Rab4 through a dual effector, RUFY1/Rabip4. Mol Biol Cell. 21, 2746-55 (2010)(PMID:20534812)
  3. Valbuena N et al. The Vam6 and Gtr1-Gtr2 pathway activates TORC1 in response to amino acids in fission yeast. J Cell Sci. 125, 1920-8 (2012)(PMID:22344254)
  4. Funabashi T et al. Ciliary entry of KIF17 is dependent on its binding to the IFT-B complex via IFT46-IFT56 as well as on its nuclear localization signal. Mol Biol Cell. 28, 624-63 (2017)(PMID:28077622)
  5. Satoh R et al. Distinct modes of stress granule assembly mediated by the KH-type RNA-binding protein Rnc1. Genes Cells. 23, 778-785 (2018)(PMID:30014536)
  6. Hirashima T et al. The ABD on the nascent polypeptide and PH domain are required for the precise Anillin localization in Drosophila syncytial blastoderm. Sci Rep. 8, 12910 (2018)(PMID:30150713)
  7. Purvanov V et al. Fluorescently Tagged CCL19 and CCL21 to Monitor CCR7 and ACKR4 Functions. Int J Mol Sci. 19, pii: E3876 (2018) (PMID:30518137)
  8. Sugasawa T et al. Detection of Transgenes in Gene Delivery Model Mice by Adenoviral Vector Using ddPCR. Genes (Basel). 10, pii: E436 (2019)(PMID:31181711)
  9. Murphy JT et al. Simple nutrients bypass the requirement for HLH-30 in coupling lysosomal nutrient sensing to survival. PLoS Biol. 17, e3000245 (2019)(PMID:31086360)
  10. Matsuda T, Oinuma I Optimized CRISPR/Cas9-mediated in vivo genome engineering applicable to monitoring dynamics of endogenous proteins in the mouse neural tissues. Sci Rep. 9, 11309 (2019)(PMID:31383899)
  11. Takano S et al. A novel system of bacterial cell division arrest implicated in horizontal transmission of an integrative and conjugative element. PLoS Genet. 15, e1008445 (2019)(PMID:31609967)
  12. Oliva Trejo JA et al. Characterization of starvation-induced autophagy in cerebellar Purkinje cells of pHluorin-mKate2-human LC3B transgenic mice. Sci Rep. 10, 9643 (2020)(PMID:32541814)
  13. Fukuda T et al. Atg43 tethers isolation membranes to mitochondria to promote starvation-induced mitophagy in fission yeast. Elife. 9, e61245 (2020)(PMID:33138913)
  14. Bueno, C et al. Rett Syndrome Mutant Neural Cells Lacks MeCP2 Immunoreactive Bands. PLoS One. 11, e0153262(2016)(PMID:27064487)

Immunocytochemistry

  1. Kennedy MJ et al. Rapid blue-light-mediated induction of protein interactions in living cells. Nat Methods. 7, 973-5 (2010)(PMID:21037589)
  2. Arimoto-Matsuzaki K et al. TIA1 oxidation inhibits stress granule assembly and sensitizes cells to stress-induced apoptosis. Nat Commun. 7, 10252 (2016)(PMID:26738979)
  3. Kitao T et al. Legionella Manipulates Non-canonical SNARE Pairing Using a Bacterial Deubiquitinase. Cell Rep. 32, 108107 (2020) (PMID:32905772)

Immunohistochemistry

  1. Humphreys BD et al. Fate tracing reveals the pericyte and not epithelial origin of myofibroblasts in kidney fibrosis. Am J Pathol. 176, 85-97 (2010)(PMID:20008127)
  2. Skora AD, Spradling AC. Epigenetic stability increases extensively during Drosophila follicle stem cell differentiation. PNAS 107, 7389-94 (2010)(PMID:20368445)
  3. Suzuki M et al. MID1 and MID2 are required for Xenopus neural tube closure through the regulation of microtubule organization. Development. 137, 2329-39 (2010)(PMID:20534674)
  4. Okumura T et al. Left-right asymmetric morphogenesis of the anterior midgut depends on the activation of a non-muscle myosin II in Drosophila. Dev Biol. 344, 693-706 (2010)(PMID:20553709)
  5. Williams SE et al. Asymmetric cell divisions promote Notch-dependent epidermal differentiation. Nature. 470, 353-8 (2011)(PMID:21331036)
  6. Letzkus JJ et al. A disinhibitory microcircuit for associative fear learning in the auditory cortex. Nature 480, 331-5 (2011)(PMID:22158104)
  7. Nakazawa N et al. A novel Cre/loxP system for mosaic gene expression in the Drosophila embryo. Dev Dyn. 241, 965-74 (2012)(PMID:22437963)
  8. Daadi MM et al. Imaging neural stem cell graft-induced structural repair in stroke. Cell Transplant. 22, 881-92 (2013)(PMID:23044338)
  9. Chen AE et al. Functional evaluation of ES cell-derived endodermal populations reveals differences between Nodal and Activin A-guided differentiation. Development. 140, 675-86 (2013)(PMID:23293299)
  10. Nagao M et al. Zbtb20 promotes astrocytogenesis during neocortical development. Nat Commun. 7, 11102 (2016)(PMID:27000654)
  11. Wang N et al. Regulation of Prostate Development and Benign Prostatic Hyperplasia by Autocrine Cholinergic Signaling via Maintaining the Epithelial Progenitor Cells in Proliferating Status. Stem Cell Reports. 6, 668-678 (2016)(PMID:27167157)
  12. Tuschl K et al. Mutations in SLC39A14 disrupt manganese homeostasis and cause childhood-onset parkinsonism-dystonia. Nat Commun. 7, 11601 (2016)(PMID:27231142)
  13. Spiró Z et al. Transcriptional enhancement of Smn levels in motoneurons is crucial for proper axon morphology in zebrafish. Sci Rep. 6, 27470 (2016)(PMID:27273160)
  14. Fujii M et al. Sfrp5 identifies murine cardiac progenitors for all myocardial structures except for the right ventricle. Nat Commun. 8, 14664 (2017)(PMID:28287088)
  15. Woodard LE et al. Kidney-specific transposon-mediated gene transfer in vivo. Sci Rep. 7, 44904 (2017)(PMID:28317878)
  16. Shainer I et al. Novel hypophysiotropic AgRP2 neurons and pineal cells revealed by BAC transgenesis in zebrafish. Sci Rep. 7, 44777 (2017)(PMID:28317906)
  17. Hoerder-Suabedissen A et al. Subset of Cortical Layer 6b Neurons Selectively Innervates Higher Order Thalamic Nuclei in Mice. Cereb Cortex. 28, 1882-1897 (2018)(PMID:29481606)
  18. Iwasaki K et al. Ablation of Central Serotonergic Neurons Decreased REM Sleep and Attenuated Arousal Response. Front Neurosci. 12, 535 (2018)(PMID:30131671)
  19. Hirashima T et al. The ABD on the nascent polypeptide and PH domain are required for the precise Anillin localization in Drosophila syncytial blastoderm. Sci Rep. 8, 12910 (2018)(PMID:30150713)
  20. Du XF et al. A transgenic zebrafish model for in vivo long-term imaging of retinotectal synaptogenesis. Sci Rep. 8, 14077 (2018)(PMID:30232367)
  21. Bin Saifullah MA et al. Cell type-specific activation of mitogen-activated protein kinase in D1 receptor-expressing neurons of the nucleus accumbens potentiates stimulus-reward learning in mice. Sci Rep. 8, 14413 (2018)(PMID:30258218)
  22. Plein A et al. Erythro-myeloid progenitors contribute endothelial cells to blood vessels. Nature 562, 223-228 (2018)(PMID:30258231)
  23. Allahyari RV et al. Sonic hedgehog signaling is negatively regulated in reactive astrocytes after forebrain stab injury. Sci Rep. 9, 565 (2019) (PMID:30679745)
  24. Cagnet S et al. Oestrogen receptor α AF-1 and AF-2 domains have cell population-specific functions in the mammary epithelium.(PMID:30413705)
  25. Knapp EM et al. Nuclear receptor Ftz-f1 promotes follicle maturation and ovulation partly via bHLH/PAS transcription factor Sim. Elife. 9, e54568 (2020)(PMID:32338596)
  26. Rai Y et al. Phospholipase C β3 is Required for Climbing Fiber Synapse Elimination in Aldolase C-positive Compartments of the Developing Mouse Cerebellum. Neuroscience. in press (PMID:32360594)
  27. Cerpa JC et al. Targeting Catecholaminergic Systems in Transgenic Rats With a CAV-2 Vector Harboring a Cre-Dependent DREADD Cassette. Front Mol Neurosci. 13, 121 (2020)(PMID:32719584)

Immunofluorescence

  1. Asakawa K et al. Optogenetic modulation of TDP-43 oligomerization accelerates ALS-related pathologies in the spinal motor neurons. Nat Commun. 11, 1004 (2020)(PMID:32081878)
  2. Hattori Y et al. Transient microglial absence assists postmigratory cortical neurons in proper differentiation. Nat Commun. 11, 1631 (2020)(PMID:32242005)
  3. Oliva Trejo JA et al. Characterization of starvation-induced autophagy in cerebellar Purkinje cells of pHluorin-mKate2-human LC3B transgenic mice. Sci Rep. 10, 9643 (2020)(PMID:32541814)

Other(Immuno-EM)

  1. Hoerder-Suabedissen A et al. Subset of Cortical Layer 6b Neurons Selectively Innervates Higher Order Thalamic Nuclei in Mice. Cereb Cortex. 28, 1882-1897 (2018)(PMID:29481606)

※在庫につきましては2024年04月20日 00時05分時点における在庫数を表示してあります。
※価格の赤色表示につきましては、キャンペーン価格の表示となっております。
※このサイトからは直接注文はできません。ご注意ください。
※使用法の表記について:
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation,
FCM: Flow Cytometry NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation,
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation,
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization

ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
※使用法・交差性の表記について:
*: 論文で報告されております(MBLでは未確認)。詳しくはデータシートをご覧ください。
**: 導入元からの情報です(MBLでは評価中もしくは未確認)。
※保存温度の表記について: RT: 室温
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