抗体 製品詳細情報

Code No. MK-11-3 プリントプリント
Anti-Ubiquitin mAb
This antibody (Clone 1B3) does not react with multi ubiquitin.
価格(税別) ¥56,000
包装 100 µg/100 µL
在庫 10以上
(2019/12/16 00:05時点)
クローナリティー Monoclonal
クローン 1B3
アイソタイプ
(免疫動物)
Mouse IgG1
使用法 WB 5 µg/mL  
IC* reported.  (PMID: 16877356 / 12388748)
IH* reported.  (PMID: 16625204 / 15866887 / 14684739)
Other(EM) reported.  (PMID: 15866887)
免疫原(抗原) Bovine erythrocyte ubiquitin
交差性 [Gene ID]

Human, Mouse*(PMID: 16625204/ 15866887), Bovine*(PMID: 10339540)

性状 1 mg/mL in PBS/50% glycerol, pH 7.2
保存温度 -20°C
標識 Unlabeled
メーカー MBL
背景 Ubiquitin is a polypeptide of 76 amino acid residues, and widely distributed protein in eukaryotic cells. This protein is also highly conserved among eukaryotic cells. There are several reports showed that intracellular abnormal and short-lived proteins are degraded through an ubiquitin dependent proteolytic pathway. In the ubiquitin dependent pathway, a target protein is tagged with multi-ubiquitin molecules.
関連製品 D058-3 Anti-Multi Ubiquitin mAb
D058-8 Anti-Multi Ubiquitin mAb-Agarose
D071-3 Anti-Multi Ubiquitin mAb
MK-12-3 Anti-Ubiquitin mAb
製品カテゴリー 分野:オートファジー ユビキチンプロテアソーム系
ブランド:使用文献・データ募集プレゼント対象製品
データ

使用文献

Western Blotting

  1. Hatakeyama S et al. Ubiquitin-dependent degradation of IkappaBalpha is mediated by a ubiquitin ligase Skp1/Cul 1/F-box protein FWD1. PNAS 96, 3859-63 (1999)(PMID:10097128)
  2. Yamanaka A et al. Cell cycle-dependent expression of mammalian E2-C regulated by the anaphase-promoting complex/cyclosome. Mol Biol Cell 11, 2821-2831 (2000)(PMID:10930472)
  3. Miyazaki K et al. NEDL1, a novel ubiquitin-protein isopeptide ligase for dishevelled-1, targets mutant superoxide dismutase-1. J Biol Chem. 279, 11327-35 (2004)(PMID:14684739)
  4. Uchida D et al. AIRE functions as an E3 ubiquitin ligase. J. Exp. Med. 199, 167-172 (2004)(PMID:14734522)
  5. Zhang HF et al. Cullin 3 promotes proteasomal degradation of the topoisomerase I-DNA covalent complex. Cancer Res. 64, 1114-21 (2004)(PMID:14871846)
  6. Akiyoshi H et al. Subcellular expression of autoimmune regulator is organized in a spatiotemporal manner. J Biol Chem. 279, 33984-91 (2004)(PMID:15150263)
  7. Hatakeyama S et al. Interaction of U-box-type ubiquitin-protein ligases (E3s) with molecular chaperones. Genes Cells. 9, 533-548 (2004)(PMID:15189447)
  8. Okumura F et al. Functional regulation of FEZ1 by the U-box-type ubiquitin ligase E4B contributes to neuritogenesis. J Biol Chem. 279, 53533-43 (2004)(PMID:15466860)
  9. Komatsu M et al. Impairment of starvation-induced and constitutive autophagy in Atg7-deficient mice. J. Cell Biol. 169, 425-34 (2005)(PMID:15866887)
  10. Waguri S, Komatsu M. Biochemical and morphological detection of inclusion bodies in autophagy-deficient mice. Methods Enzymol. 453, 181-96 (2009)(PMID:19216907)

Immunocytochemistry

  1. Nakamichi I et al. Formation of Mallory body-like inclusions and cell death induced by deregulated expression of keratin 18. Mol Biol Cell. 13, 3441-51 (2002)(PMID:12388748)
  2. Tanji K et al. NUB1 suppresses the formation of Lewy body-like inclusions by proteasomal degradation of synphilin-1. Am J Pathol. 169, 553-65 (2006)(PMID:16877356)

Immunohistochemistry

  1. Miyazaki K et al. NEDL1, a novel ubiquitin-protein isopeptide ligase for dishevelled-1, targets mutant superoxide dismutase-1. J Biol Chem. 279, 11327-35 (2004)(PMID:14684739)
  2. Komatsu M et al. Impairment of starvation-induced and constitutive autophagy in Atg7-deficient mice. J. Cell Biol. 169, 425-34 (2005)(PMID:15866887)
  3. Hara T et al. Suppression of basal autophagy in neural cells causes neurodegenerative disease in mice. Nature 441, 885-9 (2006)(PMID:16625204)
  4. Mori F et al. Valosin-containing protein immunoreactivity in tauopathies, synucleinopathies, polyglutamine diseases and intranuclear inclusion body disease. Neuropathology 33, 637-44 (2013)(PMID:23782134)
  5. Kon T et al. ALS-associated protein FIG4 is localized in Pick and Lewy bodies, and also neuronal nuclear inclusions, in polyglutamine and intranuclear inclusion body diseases. Neuropathology 34, 19-26 (2014)(PMID:23888880)
  6. Mori F et al. G protein-coupled receptor 26 immunoreactivity in intranuclear inclusions associated with polyglutamine and intranuclear inclusion body diseases. Neuropathology 36, 50-5 (2016)(PMID:26303144)
  7. Shin H et al. The formation of multivesicular bodies in activated blastocysts is influenced by autophagy and FGF signaling in mice. Sci Rep. 7, 41986 (2017)(PMID:28155881)
  8. Mori F et al. Immunohistochemical localization of exoribonucleases (DIS3L2 and XRN1) in intranuclear inclusion body disease. Neurosci Lett. 662, 389-394 (2018)(PMID:29100804)

Other(EM)

  1. Nakamichi I et al. Formation of Mallory body-like inclusions and cell death induced by deregulated expression of keratin 18. Mol Biol Cell. 13, 3441-51 (2002)(PMID:12388748)
  2. Komatsu M et al. Impairment of starvation-induced and constitutive autophagy in Atg7-deficient mice. J. Cell Biol. 169, 425-34 (2005)(PMID:15866887)

※在庫につきましては2019年12月16日 00時05分時点における在庫数を表示してあります。
※価格の赤色表示につきましては、キャンペーン価格の表示となっております。
※このサイトからは直接注文はできません。ご注意ください。
※使用法の表記について:
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation,
FCM: Flow Cytometry NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation,
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation,
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization

ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
※使用法・交差性の表記について:
*: 論文で報告されております(MBLでは未確認)。詳しくはデータシートをご覧ください。
**: 導入元からの情報です(MBLでは評価中もしくは未確認)。
※保存温度の表記について: RT: 室温
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