抗体 製品詳細情報

Code No. M207-3
Anti-MitoPLD (Pld6) mAb
Useful for WB, IP and IHC.
価格(税別) ¥52,800
包装 100 µL (1 mg/mL)
在庫 1-9
(2024/04/19 17:00時点)
クローナリティー Monoclonal
クローン 26C46-6
アイソタイプ
(免疫動物)
Mouse IgG2bκ
使用法 WB 2-5 µg/mL  
IP 3 µg/mL  
IH 10 µg/mL  
免疫原(抗原) KLH conjugated synthetic peptide, EFDPTKYSFFPQKHRGH (corresponding to amino acidresidues 205-221 of mouse MitoPLD (Pld6)
交差性 [Gene ID]

Human**, Mouse[194908], Rat**

性状 1 mg/mL in PBS/50% glycerol, pH 7.2
保存温度 -20°C
標識 Unlabeled
メーカー MBL
別称 mZuc (Zucchini), Zuc, Gm10, phospholipase D family, member 6
背景 MitoPLD (Pld6), the ortholog of drosophila Zucchini (Zuc), is a member of the phospholipase D superfamily. MitoPLD is strongly expressed in gonads especially in testis, and is located on the outer mitochondrial membrane. It is reported that MitoPLD hydrolyzes cardiolipin to generate phosphatidic acid (PA) at mitochondrial surface, and promotes mitochondrial fusion. PA, generated by MitoPLD, may work as a signaling molecule to regulate the localization and formation of mitochondria and intermitochondrial cement (nuage). It is also reported that the drosophila MitoPLD ortholog, Zuc, is an endoribonuclease essential for primary piRNA biogenesis by processing long non-coding RNA precursors. MitoPLD-/- mice show meiotic arrest during spermatogenesis, demethylation and derepression of retrotransposons, and defects in primary piRNA biogenesis, indicating that the role of MitoPLD/Zuc in the piRNA pathway is highly conserved among species.
関連製品 PM044 Anti-PIWIL2 (MILI) (Mouse) pAb
D289-3 Anti-Np95 (Uhrf1) (Mouse) mAb
M202-3 Anti-Np95 (Uhrf1) mAb
RN1005 RIP-Assay Kit for microRNA
D356-3 Anti-Jmjd1c (Mouse) mAb
M207-3 Anti-MitoPLD (Pld6) mAb
RN125PW Anti-HENMT1 pAb
D357-3 Anti-Ace (CD143) (Mouse) mAb
D363-3 Anti-Ly6k (Mouse) mAb
D365-3 Anti-PIWIL2 (MILI) (Mouse) mAb
製品カテゴリー 分野:幹細胞研究 RNA-RNPネットワーク エピジェネティクス
データ



使用文献

Western Blotting

  1. Kabayama Y et al. Roles of MIWI, MILI and PLD6 in small RNA regulation in mouse growing oocytes. Nucleic Acids Res. 45, 5387-5398 (2017)(PMID:28115634)
参考文献
  1. Nishimasu H et al. Structure and function of Zucchini endoribonuclease in piRNA biogenesis. Nature. 491, 284-7 (2012)(PMID: 23064230
  2. Gao Q, Frohman MA. Roles for the lipid-signaling enzyme MitoPLD in mitochondrial dynamics, piRNA biogenesis, and spermatogenesis. BMB Rep. 45, 7-13 (2012)(PMID: 22281006
  3. Huang H et al. piRNA-associated germline nuage formation and spermatogenesis require MitoPLD profusogenic mitochondrial-surface lipid signaling. Dev Cell. 20, 376-87 (2011)(PMID: 21397848
  4. Watanabe T et al. MITOPLD is a mitochondrial protein essential for nage formation and piRNA biogenesis in the mouse germline. Dev Cell. 20, 364-75 (2011)(PMID: 21397847
  5. Choi SY et al. A common lipid links Mfn-mediated mitochondrial fusion and SNARE-regulated exocytosis. Nat Cell Biol. 8, 1255-62 (2006)(PMID: 17028579

※在庫につきましては2024年04月19日 17時00分時点における在庫数を表示してあります。
※価格の赤色表示につきましては、キャンペーン価格の表示となっております。
※このサイトからは直接注文はできません。ご注意ください。
※使用法の表記について:
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation,
FCM: Flow Cytometry NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation,
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation,
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization

ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
※使用法・交差性の表記について:
*: 論文で報告されております(MBLでは未確認)。詳しくはデータシートをご覧ください。
**: 導入元からの情報です(MBLでは評価中もしくは未確認)。
※保存温度の表記について: RT: 室温
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