抗体 製品詳細情報

Code No. M162-3
Anti-p62 (SQSTM1) (Human) mAb
価格(税別) ¥52,800
包装 100 µL (1 mg/mL)
在庫 10以上
（2024/10/05 17:00時点）
クローナリティー Monoclonal
クローン 5F2
アイソタイプ
(免疫動物)		 Mouse IgG1 κ
使用法 WB　1 µg/mL  
IP　2 µg/250 µL of cell extract from 2.5x106 cells  
FCM　2 µg/mL  
IC　5 µg/mL  
IH　2-10 µg/mL (Heat treatment is necessary for paraffin embedded sections.)  
免疫原(抗原) Recombinant Human p62 (120-440 a.a.)
交差性 [Gene ID]

Human[8878], Mouse(-), Rat(-), Hamster(-)
性状 1 mg/mL in PBS/50% glycerol, pH 7.2
保存温度 -20°C
標識 Unlabeled
メーカー MBL
別称 sequestosome 1, p60, p62, A170, OSIL, PDB3, ZIP3, p62B
背景 p62/SQSTM1 interacts with various molecular groups such as RIP, TRAF6, ERK, aPKCs, and poly-ubiquitin through PB1 domain, Zn finger domain, and UBA domain. This protein directly interacts with LC3, which is localized on autophagosome membrane, and is degradated by autophagic-lysosome pathway. p62 regulates ubiquitin-positive protein aggregates caused by autophagy deficiency.
関連製品 PM045 Anti-p62 (SQSTM1) pAb
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PM066 Anti-p62 C-terminal pAb
PM036 Anti-LC3 pAb
M152-3 Anti-LC3 mAb
M186-3 Anti-LC3 mAb
PD014 Anti-LC3 pAb
PM069 Anti-NRF2 pAb
PM072 Anti-VMP1 pAb
8485 Autophagy Ab Sampler Set
PM090 Anti-Atg8 (Filamentous fungi) pAb
D343-3 Anti-Phospho-p62 (SQSTM1) (Ser403) mAb
D344-3 Anti-Phospho-p62 (SQSTM1) (Ser403) mAb
PM074 Anti-Phospho-p62 (SQSTM1) (Ser351) pAb
M224-3 Anti-KEAP1 mAb
M200-3 Anti-NRF2 mAb
D343-3MS Anti-Phospho-p62 (SQSTM1) (Ser403) mAb for My select sampler set
D344-3MS Anti-Phospho-p62 (SQSTM1) (Ser403) mAb for My select sampler set
M152-3MS Anti-LC3 mAb for My select sampler set
PM074MS Anti-Phospho-p62 (SQSTM1) (Ser351) pAb for My select sampler set
M230-3 Anti-Parkin mAb
PM037 Anti-GABARAP pAb
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M135-3 Anti-GABARAP mAb
M217-3 Anti-Phospho-p62 (SQSTM1) (Ser351) mAb
M217-3MS Anti-Phospho-p62 (SQSTM1) (Ser351) mAb for My select sampler set
PM038 Anti-GATE-16 pAb
MK-12-3 Anti-Ubiquitin mAb
CY-7055 CycLex® Total p62 ELISA Kit
製品カテゴリー 分野:オートファジー
データ





使用文献

Western Blotting

  1. Soll C et al. Serotonin promotes tumor growth in human hepatocellular cancer. Hepatology 51, 1244-54 (2010)(PMID:20099302)
  2. Fan W et al. Keap1 facilitates p62-mediated ubiquitin aggregate clearance via autophagy. Autophagy 6, 614-21 (2010)(PMID:20495340)
  3. Wang BS et al. Autophagy negatively regulates cancer cell proliferation via selectively targeting VPRBP. Clin Sci (Lond). 124, 203-14 (2013)(PMID:22963397)
  4. Rovetta F et al. ER signaling regulation drives the switch between autophagy and apoptosis in NRK-52E cells exposed to cisplatin. Exp Cell Res. 318, 238-50 (2012)(PMID:22146761)
  5. Matsumoto G et al. Serine 403 phosphorylation of p62/SQSTM1 regulates selective autophagic clearance of ubiquitinated proteins. Mol Cell. 44, 279-89 (2011)(PMID:22017874)
  6. Schmeisser H et al. Type I interferons induce autophagy in certain human cancer cell lines. Autophagy 9, 683-96 (2013)(PMID:23419269)
  7. Guo X et al. Patulin induces pro-survival functions via autophagy inhibition and p62 accumulation. Cell Death Dis. 4, e822 (2013)(PMID:24091665)
  8. Zhu X et al. Cytosolic HMGB1 controls the cellular autophagy/apoptosis checkpoint during inflammation. J Clin Invest. 125, 1098-110 (2015)(PMID:25642769)
  9. Janda E et al. Parkinsonian toxin-induced oxidative stress inhibits basal autophagy in astrocytes via NQO2/quinone oxidoreductase 2: Implications for neuroprotection. Autophagy 11, 1063-80 (2015)(PMID:26046590)
  10. Stolz A et al. Fluorescence-based ATG8 sensors monitor localization and function of LC3/GABARAP proteins. EMBO J. 36, 549-564 (2017)(PMID:28028054)
  11. Sakamoto S et al. Mild MPP+ exposure-induced glucose starvation enhances autophagosome synthesis and impairs its degradation. Sci Rep. 7, 46668 (2017)(PMID:28443637)
  12. Wacker R et al. Fluorescence-based ATG8 sensors monitor localization and function of LC3/GABARAP proteins. Cell Microbiol. 19, e12754 (2017)(PMID:28573684)
  13. Bingel C et al. Three-dimensional tumor cell growth stimulates autophagic flux and recapitulates chemotherapy resistance. Cell Death Dis. 8, e3013 (2017)(PMID:28837150)
  14. Schmuckli-Maurer J et al. Inverted recruitment of autophagy proteins to the Plasmodium berghei parasitophorous vacuole membrane. PLoS One. 12, e0183797 (2017)(PMID:28841718)
  15. Lötzerich M et al. Rhinovirus 3C protease suppresses apoptosis and triggers caspase-independent cell death. Cell Death Dis. 9, 272 (2018)(PMID:29449668)
  16. Sun Y et al. 3′-epi-12β-hydroxyfroside, a new cardenolide, induces cytoprotective autophagy via blocking the Hsp90/Akt/mTOR axis in lung cancer cells. Theranostics 8, 2044-2060 (2018)(PMID:29556372)
  17. Kanda R et al. Expression of the glucagon-like peptide-1 receptor and its role in regulating autophagy in endometrial cancer. BMC Cancer 18, 657 (2018)(PMID:29907137)
  18. Qiu J et al. Distinct subgroup of the Ras family member 3 (DIRAS3) expression impairs metastasis and induces autophagy of gastric cancer cells in mice. J Cancer Res Clin Oncol. 144, 1869-1886 (2018)(PMID:30043279)
  19. Mori H et al. Induction of selective autophagy in cells replicating hepatitis C virus genome. J Gen Virol. 99, 1643-1657 (2018)(PMID:30311874)
  20. Nakashima A et al. Endoplasmic reticulum stress disrupts lysosomal homeostasis and induces blockade of autophagic flux in human trophoblasts. Sci Rep. 9, 11466 (2019)(PMID:31391477)
  21. Ishii S et al. Identification of a factor controlling lysosomal homeostasis using a novel lysosomal trafficking probe. Sci Rep. 9, 11635 (2019)(PMID:31406198)
  22. Li S et al. CACNA1H downregulation induces skeletal muscle atrophy involving endoplasmic reticulum stress activation and autophagy flux blockade. Cell Death Dis. 11, 279 (2020)(PMID:32332705)
  23. Yamamuro T et al. Age-dependent loss of adipose Rubicon promotes metabolic disorders via excess autophagy. Nat Commun. 11, 4150 (2020) (PMID:32811819)

Immunoprecipitation

  1. Wang BS et al. Autophagy negatively regulates cancer cell proliferation via selectively targeting VPRBP. Clin Sci (Lond). 124, 203-14 (2013)(PMID:22963397)

Immunocytochemistry

  1. Akaishi R et al. Autophagy in the placenta of women with hypertensive disorders in pregnancy. Placenta 35, 974-80 (2014)(PMID:25465707)
  2. Janda E et al. Parkinsonian toxin-induced oxidative stress inhibits basal autophagy in astrocytes via NQO2/quinone oxidoreductase 2: Implications for neuroprotection. Autophagy 11, 1063-80 (2015)(PMID:26046590)
  3. Schmuckli-Maurer J et al. Inverted recruitment of autophagy proteins to the Plasmodium berghei parasitophorous vacuole membrane. PLoS One. 12, e0183797 (2017)(PMID:28841718)
  4. Zhao Q et al. Dual Roles of Two Isoforms of Autophagy-related Gene ATG10 in HCV-Subgenomic replicon Mediated Autophagy Flux and Innate Immunity. Sci Rep. 7, 11250 (2017)(PMID:28900156)
  5. Doerflinger SY et al. Membrane alterations induced by nonstructural proteins of human norovirus. PLoS Pathog. 13, e1006705 (2017)(PMID:29077760)
  6. Nakashima A et al. Endoplasmic reticulum stress disrupts lysosomal homeostasis and induces blockade of autophagic flux in human trophoblasts. Sci Rep. 9, 11466 (2019)(PMID:31391477)

Immunohistochemistry

  1. Wang BS et al. Autophagy negatively regulates cancer cell proliferation via selectively targeting VPRBP. Clin Sci (Lond). 124, 203-14 (2013)(PMID:22963397)
  2. Park JM et al. Prognostic impact of Beclin 1, p62/sequestosome 1 and LC3 protein expression in colon carcinomas from patients receiving 5-fluorouracil as adjuvant chemotherapy. Cancer Biol Ther. 14, 100-7 (2013)(PMID:23192274)
  3. Onodera Y et al. NRF2 immunolocalization in human breast cancer patients as a prognostic factor. Endocr Relat Cancer. 21, 241-52 (2014)
  4. Akaishi R et al. Autophagy in the placenta of women with hypertensive disorders in pregnancy. Placenta 35, 974-80 (2014)(PMID:25465707)
  5. Ishii S et al. Identification of a factor controlling lysosomal homeostasis using a novel lysosomal trafficking probe. Sci Rep. 9, 11635 (2019)(PMID:31406198)

※在庫につきましては2024年10月05日 17時00分時点における在庫数を表示してあります。
※価格の赤色表示につきましては、キャンペーン価格の表示となっております。
※このサイトからは直接注文はできません。ご注意ください。
※使用法の表記について：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation,
FCM: Flow Cytometry NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation,
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation,
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
※使用法・交差性の表記について：
*: 論文で報告されております（MBLでは未確認）。詳しくはデータシートをご覧ください。
**: 導入元からの情報です（MBLでは評価中もしくは未確認）。
※保存温度の表記について： RT: 室温
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