抗体 製品詳細情報

Code No. M150-3
Anti-Atg16L mAb
価格(税別) ¥52,800
包装 100 µL (1 mg/mL)
在庫 10以上
（2024/04/20 00:05時点）
クローナリティー Monoclonal
クローン 1F12
アイソタイプ
(免疫動物)		 Mouse IgG1 κ
使用法 WB　1 µg/mL  
FCM*　reported.  （PMID: 25597631）
IH*　reported.  （PMID: 24089213）
免疫原(抗原) Recombinant Human ATG16L1 TV2 (85-588 a.a.)
交差性 [Gene ID]

Human[55054], Mouse[77040], Rat[363278]
性状 1 mg/mL in PBS/50% glycerol, pH 7.2
保存温度 -20°C
標識 Unlabeled
メーカー MBL
別称 ATG16L1, autophagy related 16-like 1 (S. cerevisiae), IBD10, WDR30, APG16L, ATG16A, Apg16l
背景 Autophagy is a process of intracellular bulk degradation in which cytoplasmic components including organelles are sequestered within double-membrane vesicles that deliver the contents to the lysosome/vacuole for degradation. Autophagy has two ubiquitin-like conjugation systems, the Atg12 and LC3-II systems. In the Atg12 conjugation system, the Atg16L-Atg12-Atg5 forms 800 kDa complex that elongates autophagic isolation membrane. After completion of the formation of the autophagosome, the Atg12-Atg5-Atg16L complex dissociates from the membrane. In recent study, nonsynonymous SNP analysis has indicated that ATG16L1 is a Crohn's disease susceptibility gene.
関連製品 8485 Autophagy Ab Sampler Set
PM040 Anti-Atg16L pAb
M151-3 Anti-Atg10 (Human) mAb
M154-3 Anti-Atg12 (Human) mAb
M153-3 Anti-Atg5 mAb
PM050 Anti-Atg5 pAb
PM036 Anti-LC3 pAb
M152-3 Anti-LC3 mAb
PD017 Anti-Beclin 1 pAb
PM072 Anti-VMP1 pAb
PM045 Anti-p62 (SQSTM1) pAb
M133-3 Anti-Atg3 mAb
PM039 Anti-Atg7 (Human) pAb
製品カテゴリー 分野:オートファジー
データ

使用文献

Western Blotting

  1. Adolph TE et al. Paneth cells as a site of origin for intestinal inflammation. Nature 503, 272-6 (2013)(PMID:24089213)
  2. Myeku N et al. Dynamics of the degradation of ubiquitinated proteins by proteasomes and autophagy: association with sequestosome 1/p62. J Biol Chem 286, 22426-40 (2011)(PMID:21536669)
  3. Murthy A et al. A Crohn's disease variant in Atg16l1 enhances its degradation by caspase 3. Nature 506, 456-62 (2014)(PMID:24553140)
  4. Boada-Romero E et al. The T300A Crohn's disease risk polymorphism impairs function of the WD40 domain of ATG16L1. Nat Commun. 7, 11821 (2016)(PMID:27273576)
  5. Diamanti MA et al. IKKα controls ATG16L1 degradation to prevent ER stress during inflammation. J Exp Med. 214, 423-437 (2017)(PMID:28082356)
  6. Slowicka K et al. Physical and functional interaction between A20 and ATG16L1-WD40 domain in the control of intestinal homeostasis. Nat Commun. 10, 1834 (2019)(PMID:31015422)
  7. Shizukuishi S et al. Streptococcus pneumoniae hijacks host autophagy by deploying CbpC as a decoy for Atg14 depletion. EMBO Rep. 21, e49232 (2020)(PMID:32239622)

Immunoprecipitation

  1. Serramito-Gómez I et al. Regulation of cytokine signaling through direct interaction between cytokine receptors and the ATG16L1 WD40 domain. Nat Commun. 11, 5919 (2020)(PMID:33219218)

Flow Cytometry

  1. Morozova K et al. Annexin A2 promotes phagophore assembly by enhancing Atg16L+ vesicle biogenesis and homotypic fusion. Nat Commun. 6, 5856 (2015)(PMID:25597631)

Immunocytochemistry

  1. Kucharewicz K et al. Simultaneous induction and blockade of autophagy by a single agent. Cell Death Dis. 9, 353 (2018)(PMID:29500364)
  2. Chu J et al. ATG4B inhibitor FMK-9a induces autophagy independent on its enzyme inhibition. Arch Biochem Biophys. 644, 29-36 (2018)(PMID:29510087)

Immunohistochemistry

  1. Adolph TE et al. Paneth cells as a site of origin for intestinal inflammation. Nature 503, 272-6 (2013)(PMID:24089213)
  2. Asai E et al. Spatiotemporal alterations of autophagy marker LC3 in rat skin fibroblasts during wound healing process. Fukushima J Med Sci. 64, 15-22 (2018)(PMID:29343655)

Immunofluorescence

  1. Morozova K et al. Annexin A2 promotes phagophore assembly by enhancing Atg16L+ vesicle biogenesis and homotypic fusion. Nat Commun. 6, 5856 (2015)(PMID:25597631)

※在庫につきましては2024年04月20日 00時05分時点における在庫数を表示してあります。
※価格の赤色表示につきましては、キャンペーン価格の表示となっております。
※このサイトからは直接注文はできません。ご注意ください。
※使用法の表記について：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation,
FCM: Flow Cytometry NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation,
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation,
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
※使用法・交差性の表記について：
*: 論文で報告されております（MBLでは未確認）。詳しくはデータシートをご覧ください。
**: 導入元からの情報です（MBLでは評価中もしくは未確認）。
※保存温度の表記について： RT: 室温
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