抗体 製品詳細情報

Code No. D291-7
Anti-His-tag mAb-HRP-DirecT
価格(税別) ¥24,200
包装 50 µL
在庫 10以上
（2024/04/24 00:05時点）
クローナリティー Monoclonal
クローン OGHis
アイソタイプ
(免疫動物)		 Mouse IgG2aκ
使用法 WB　1:5,000  
免疫原(抗原) 6xHis tagged protein
性状 PBS/Preservative/Stabilizer
保存温度 4°C
標識 HRP
メーカー MBL
背景 The His-tag (6xHis-tag) is one of the most common tags used to facilitate the purification of recombinant proteins. Metal chelate affinity chromatography is widely used for purification of His-tagged proteins. This specific antibody is useful tool for monitoring of the His-tagged proteins, and recognizes His-tags placed at N-terminal, C-terminal, and internal regions of the recombinant proteins.
関連製品 PM032 Anti-His-tag pAb
PM032-8 Anti-His-tag pAb-Agarose
D291-3 Anti-His-tag mAb
D291-6 Anti-His-tag mAb-Biotin
D291-8 Anti-His-tag mAb-Agarose
D291-10 Anti-His-tag mAb-Magnetic Agarose
D291-A48 Anti-His-tag mAb-Alexa Fluor™ 488
D291-A59 Anti-His-tag mAb-Alexa Fluor™ 594
D291-A64 Anti-His-tag mAb-Alexa Fluor™ 647
M089-3 Anti-His-tag mAb
M136-3 Anti-His-tag mAb
D291-11 Anti-His-tag mAb-Magnetic Beads
製品カテゴリー ツール:エピトープタグ
データ

使用文献
  1. Lin Q et al. The SnRK2-APC/C(TE) regulatory module mediates the antagonistic action of gibberellic acid and abscisic acid pathways. Nat Commun. 6, 7981 (2015)(PMID:26272249)

Western Blotting

  1. Yamaguchi A et al. In vitro characterization of the RS motif in N-terminal head domain of goldfish germinal vesicle lamin B3 necessary for phosphorylation of the p34cdc2 target serine by SRPK1. FEBS Open Bio. 3, 165-76 (2013)(PMID:23772390)
  2. Sakata K et al. HCV NS3 protease enhances liver fibrosis via binding to and activating TGF-β type I receptor. Sci Rep. 3, 3243 (2013).(PMID:24263861)
  3. Maki M et al. Transglutaminase-catalyzed incorporation of polyamines masks the DNA-binding region of the transcription factor Relish. J Biol Chem. 292, 6369-6380 (2017)(PMID:28258224)
  4. Shibata T et al. Drosophila TG-A transglutaminase is secreted via an unconventional Golgi-independent mechanism involving exosomes and two types of fatty acylations. J Biol Chem. 292, 10723-10734 (2017)(PMID:28476891)
  5. Takeya T et al. A Pichia pastoris single-cell biosensor for detection of enzymatically produced methanol. Appl Microbiol Biotechnol. 102, 7017-7027 (2018)(PMID:29948115)
  6. Nakamura M et al. Functional characterization of unique enzymes in Xanthomonas euvesicatoria related to degradation of arabinofurano-oligosaccharides on hydroxyproline-rich glycoproteins. PLoS One 13, e0201982 (2018)(PMID:30092047)
  7. Nakashima K et al. Chitin-based barrier immunity and its loss predated mucus-colonization by indigenous gut microbiota. Nat Commun. 9, 3402 (2018) (PMID:30143642)
  8. Umehara T et al. Lysine Acetylation Regulates Alanyl-tRNA Synthetase Activity in Escherichia coli. Genes 9, E473 (2018)(PMID:30274179)
  9. Taira J et al. Dephosphorylation of clustered phosphoserine residues in human Grb14 by protein phosphatase 1 and its effect on insulin receptor complex formation. J Pept Sci. 25, e3207 (2019)(PMID:31347216)
  10. Sekimukai H et al. Gold nanoparticle-adjuvanted S protein induces a strong antigen-specific IgG response against severe acute respiratory syndrome-related coronavirus infection, but fails to induce protective antibodies and limit eosinophilic infiltration in lungs. Microbiol Immunol. 64, 33-51 (2019) (PMID:31692019)
  11. Kudo H et al. Improving Hydrocarbon Production by Engineering Cyanobacterial Acyl-(Acyl Carrier Protein) Reductase. Biotechnol Biofuels. 12, 291 (2019)(PMID:31890019)
  12. Xie Y et al. Arabidopsis FHY3 and FAR1 integrate light and strigolactone signaling to regulate branching. Nat Commun. 11, 1955 (2020)(PMID:32327664)
  13. Satomura T et al. Characterization of dye-linked d-amino acid dehydrogenase from Sulfurisphaera tokodaii expressed using an archaeal recombinant protein expression system. J Biosci Bioeng. 130, 247-252 (2020)(PMID:32451245)

ELISA

  1. Sila-On D et al. Development of a dual monoclonal antibody sandwich enzyme-linked immunosorbent assay for the detection of swine influenza virus using rabbit monoclonal antibody by Ecobody technology. J Biosci Bioeng. 130, 217-225 (2020)(PMID:32284304)

※在庫につきましては2024年04月24日 00時05分時点における在庫数を表示してあります。
※価格の赤色表示につきましては、キャンペーン価格の表示となっております。
※このサイトからは直接注文はできません。ご注意ください。
※使用法の表記について：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation,
FCM: Flow Cytometry NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation,
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation,
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
※使用法・交差性の表記について：
*: 論文で報告されております（MBLでは未確認）。詳しくはデータシートをご覧ください。
**: 導入元からの情報です（MBLでは評価中もしくは未確認）。
※保存温度の表記について： RT: 室温
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