抗体 製品詳細情報

Code No. D278-5 プリントプリント
Anti-GP2 (Glycoprotein 2) (Mouse) mAb-PE
価格(税別) ¥40,000
包装 1 mL
在庫 10以上
(2022/08/10 17:30時点)
クローナリティー Monoclonal
クローン 2F11-C3
アイソタイプ
(免疫動物)
Rat IgG2aκ
使用法 FCM 1:10  
免疫原(抗原) Mouse GP2, extracellular domain (recombinant, human Fc fusion protein)
交差性 [Gene ID]

Human(-), Mouse[67133]

性状 PBS/1% BSA/0.1% ProClin 150
保存温度 4°C
標識 PE
メーカー MBL
別称 glycoprotein 2 (zymogen granule membrane)
背景 M cells are located in the follicle associated epithelium (FAE) of Peyer’s patches (PPs) in the small intestine, where they mediate the uptake and transcytosis of luminal antigens to the underlying lymphoid tissue. Gycoprotein 2 (GP2) is a GPI-anchor protein that was previously thought to be exclusively expressed by pancreatic acinar cells of most mammals. Recent studies reported that GP2 is specifically expressed in M cells among intestinal epithelial cells, and serves as a transcytotic receptor for mucosal antigens. Although UEA-1 (Ulex europaeus agglutinin-1) lectin has been widely applied to detect M cells, this lectin also stains goblet cells. Furthermore, UEA-1 can only bind mouse, but not human, M cells. Thus, GP2 is the first universal M-cell marker in mouse and human. Mouse and human GP2 recombinant proteins selectively bind a subset of commensal and pathogenic enterobacteria, including Escherichia coli and Salmonella spp., by recognizing FimH-expressing type-1-pili on the bacterial outer membrane. GP2 expressed on apical surface of M cells serves as a receptor for type-1-piliated bacteria, and facilitates translocation of these bacteria from the intestinal lumen to lymphoid follicles. Consequently, antigen-specific mucosal immune response to these bacteria is induced. Thus, GP2 plays an important role in mucosal immunosurveillance. Given that M cell are considered a promising target for oral vaccination against diverse infectious diseases, the GP2-dependent transcytotic pathway could provide a new target for development of M-cell-targeted mucosal vaccines.
関連製品 D277-3 Anti-GP2 (Glycoprotein 2) (Human) mAb
D278-3 Anti-GP2 (Glycoprotein 2) (Mouse) mAb
D278-A48 Anti-GP2 (Glycoprotein 2) (Mouse) mAb-Alexa Fluor® 488
D278-6 Anti-GP2 (Glycoprotein 2) (Mouse) mAb-Biotin
D277-A48 Anti-GP2 (Glycoprotein 2) (Human) mAb-Alexa Fluor® 488
D277-5 Anti-GP2 (Glycoprotein 2) (Human) mAb-PE
製品カテゴリー 分野:免疫
データ

使用文献

Immunofluorescence

  1. Ramakrishnan SK et al. Intestinal non-canonical NFκB signaling shapes the local and systemic immune response. Nat Commun. 10, 660 (2019)(PMID:30737385)

※在庫につきましては2022年08月10日 17時30分時点における在庫数を表示してあります。
※価格の赤色表示につきましては、キャンペーン価格の表示となっております。
※このサイトからは直接注文はできません。ご注意ください。
※使用法の表記について:
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation,
FCM: Flow Cytometry NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation,
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation,
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization

ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
※使用法・交差性の表記について:
*: 論文で報告されております(MBLでは未確認)。詳しくはデータシートをご覧ください。
**: 導入元からの情報です(MBLでは評価中もしくは未確認)。
※保存温度の表記について: RT: 室温
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