抗体 製品詳細情報

Code No. D210-3 プリントプリント
Anti-Histone H2A mAb
価格(税別) ¥48,000
包装 100 µg/100 µL
在庫 10以上
(2020/06/06 00:05時点)
クローナリティー Monoclonal
クローン C10037
アイソタイプ
(免疫動物)
Mouse IgG2b
使用法 WB 1 µg/mL  
IP 2 µg/200 µL of cell extract from 5x106 cells  
IC 1 µg/mL  
免疫原(抗原) Protein fraction extracted from the mitotic chromosome
交差性 [Gene ID]

Human, Mouse, Rat, Hamster

性状 1 mg/mL in PBS/50% glycerol, pH 7.2
保存温度 -20°C
標識 Unlabeled
メーカー MBL
背景 The nucleosome is made up of four core histone proteins (H2A, H2B, H3 and H4) and is the primary building block of chromatin. The N-terminal tail of core histones undergoes multiple different post-translational modifications including acetylation, phosphorylation, ubiquitination, methylation and ADP-ribosylation. These modifications occur in response to cell signal stimuli and have a direct effect on gene expression. The role of histone variants, and specially those of H3 and H2A, in various nuclear processes has been long appreciated. There are at least three different families of H2A variants present in a variety of organisms from yeast to mammals, and the degree of conservation among members of each family is greater than that of the canonical H2A. H2AX is thought to play a role in DNA double-strand break repair; the serine in the SQEY motif of H2AX is phosphorylated at the site of the DNA damage and serves as a signal for the recruitment of repair proteins. Macro H2A1, another H2A variant, has been shown to have a role in X-chromosome inactivation and dosage compensation in mammals, where it is found to localize to the inactive X after silencing has been established.
関連製品 D212-3 Anti-Histone H2B mAb
D214-3 Anti-Histone H4 mAb
D139-3 Anti-Ring1B mAb
製品カテゴリー :分野:エピジェネティクス がん
データ

使用文献
  1. Suzuki Y et al. Nuclear formation induced by DNA-conjugated beads in living fertilised mouse egg. Sci Rep. 9, 8461 (2019)(PMID:31186495)

※在庫につきましては2020年06月06日 00時05分時点における在庫数を表示してあります。
※価格の赤色表示につきましては、キャンペーン価格の表示となっております。
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※使用法の表記について:
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation,
FCM: Flow Cytometry NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation,
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation,
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization

ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
※使用法・交差性の表記について:
*: 論文で報告されております(MBLでは未確認)。詳しくはデータシートをご覧ください。
**: 導入元からの情報です(MBLでは評価中もしくは未確認)。
※保存温度の表記について: RT: 室温
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