抗体 製品詳細情報

Code No. D147-3
Anti-Abi-1 (Abl interactor 1) mAb
価格(税別) ¥44,000
包装 100 µL (1 mg/mL)
在庫 10以上
（2024/04/19 00:05時点）
クローナリティー Monoclonal
クローン 1B9
アイソタイプ
(免疫動物)		 Mouse IgG1
使用法 WB　1 µg/mL  
IP　2 µg/200 µL of cell extract from 5x106 cells  
IC*　reported.  （PMID: 16280551 / 21464958 / 22808230）
IH　5 µg/mL (Heat treatment is necessary for paraffin embedded sections.)  
免疫原(抗原) Recombinant Human Abi-1 expressed in E.coli.
交差性 [Gene ID]

Human[10006], Mouse[11308], Rat*[79249](PMID: 21464958/ 22102872), Hamster[100772463], Dog*[607247](PMID: 17164293)
性状 1 mg/mL in PBS/50% glycerol, pH 7.2
保存温度 -20°C
標識 Unlabeled
メーカー MBL
別称 abl-interactor 1, E3B1, ABI-1, ABLBP4, NAP1BP, SSH3BP, SSH3BP1
背景 Abl interactor (Abi) proteins were identified as a c-Abl-binding protein that bind to both the Src homology 3 (SH3) domain and the C-terminal proline-rich regions of Abl kinase through dual SH3-PXXP interactions. Two highly related genes, abi-1 and abi-2, were cloned. In addition to the interaction with Abl kinase, Abi proteins also interact with other signaling molecules. Recently, it has been reported that Abi-1 dramatically promoted c-Abl-mediated tyrosine (Tyr296) phosphorylation of Mena [mammalian homologue of Drosophila Enabled (Ena)] by interacting with both proteins.
製品カテゴリー 分野:がん シグナル伝達
データ

使用文献

Western Blotting

  1. OdaA et al. WAVE/Scars in platelets. Blood 105, 3141-8 (2005)(PMID:15280206)
  2. Kheir WA et al. A WAVE2-Abi1 complex mediates CSF-1-induced F-actin-rich membrane protrusions and migration in macrophages. J Cell Sci. 118, 5369-79 (2005)(PMID:16280551)
  3. Yamazaki D et al. Rac-WAVE-mediated actin reorganization is required for organization and maintenance of cell-cell adhesion. J Cell Sci. 120, 86-100 (2007)(PMID:17164293)
  4. Eto K et al. The WAVE2/Abi1 complex differentially regulates megakaryocyte development and spreading: implications for platelet biogenesis and spreading machinery. Blood 110, 3637-47 (2007)(PMID:17664349)
  5. Liebau S et al. An SK3 channel/nWASP/Abi-1 complex is involved in early neurogenesis. PLoS One. 6, e18148 (2011)(PMID:21464958)
  6. Proepper C et al. Heterogeneous nuclear ribonucleoprotein k interacts with Abi-1 at postsynaptic sites and modulates dendritic spine morphology. PLoS One. 6, e27045 (2011)(PMID:22102872)
  7. Steinestel K et al. Expression of Abelson interactor 1 (Abi1) correlates with inflammation, KRAS mutation and adenomatous change during colonic carcinogenesis. PLoS One. 7, e40671 (2012)(PMID:22808230)
  8. Xiong X et al. Disruption of Abi1/Hssh3bp1 expression induces prostatic intraepithelial neoplasia in the conditional Abi1/Hssh3bp1 KO mice. Oncogenesis 1, e26 (2012)(PMID:23552839)
  9. Steinestel K et al. Expression and Y435-phosphorylation of Abelson interactor 1 (Abi1) promotes tumour cell adhesion, extracellular matrix degradation and invasion by colorectal carcinoma cells. Mol Cancer. 13, 145 (2014)(PMID:24913355)
  10. Yu X et al. Inhibitory short peptides targeting EPS8/ABI1/SOS1 tri-complex suppress invasion and metastasis of ovarian cancer cells. BMC Cancer. 19, 878 (2019)(PMID:31488087)
  11. Qi Y et al. PTEN suppresses epithelial-mesenchymal transition and cancer stem cell activity by downregulating Abi1. Sci Rep. 10, 12685 (2020) (PMID:32728066)

Immunoprecipitation

  1. OdaA et al. WAVE/Scars in platelets. Blood 105, 3141-8 (2005)(PMID:15280206)
  2. Kheir WA et al. A WAVE2-Abi1 complex mediates CSF-1-induced F-actin-rich membrane protrusions and migration in macrophages. J Cell Sci. 118, 5369-79 (2005)(PMID:16280551)
  3. Sekino S et al. The NESH/Abi-3-based WAVE2 complex is functionally distinct from the Abi-1-based WAVE2 complex. Cell Commun Signal. 13, 41 (2015)(PMID:26428302)

Immunocytochemistry

  1. Kheir WA et al. A WAVE2-Abi1 complex mediates CSF-1-induced F-actin-rich membrane protrusions and migration in macrophages. J Cell Sci. 118, 5369-79 (2005)(PMID:16280551)
  2. Liebau S et al. An SK3 channel/nWASP/Abi-1 complex is involved in early neurogenesis. PLoS One. 6, e18148 (2011)(PMID:21464958)
  3. Steinestel K et al. Expression and Y435-phosphorylation of Abelson interactor 1 (Abi1) promotes tumour cell adhesion, extracellular matrix degradation and invasion by colorectal carcinoma cells. Mol Cancer. 13, 145 (2014)(PMID:24913355)

Immunohistochemistry

  1. Steinestel K et al. Expression of Abelson interactor 1 (Abi1) correlates with inflammation, KRAS mutation and adenomatous change during colonic carcinogenesis. PLoS One. 7, e40671 (2012)(PMID:22808230)
  2. Steinestel K et al. Expression and Y435-phosphorylation of Abelson interactor 1 (Abi1) promotes tumour cell adhesion, extracellular matrix degradation and invasion by colorectal carcinoma cells. Mol Cancer. 13, 145 (2014)(PMID:24913355)

※在庫につきましては2024年04月19日 00時05分時点における在庫数を表示してあります。
※価格の赤色表示につきましては、キャンペーン価格の表示となっております。
※このサイトからは直接注文はできません。ご注意ください。
※使用法の表記について：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation,
FCM: Flow Cytometry NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation,
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation,
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
※使用法・交差性の表記について：
*: 論文で報告されております（MBLでは未確認）。詳しくはデータシートをご覧ください。
**: 導入元からの情報です（MBLでは評価中もしくは未確認）。
※保存温度の表記について： RT: 室温
※本サイトの掲載製品は、品質改良のため予告なく仕様などの変更、または販売中止となる場合がございます。あらかじめご了承ください。

MBLCopyright (C)2015 Medical ＆ Biological Laboratories Co., Ltd. All Rights Reserve