抗体 製品詳細情報

Code No. D031-3
Anti-Proton Pump (H,K-ATPase α subunit) mAb
価格(税別) ¥48,000
包装 100 µL (1 mg/mL)
在庫 10以上
（2024/04/19 17:00時点）
クローナリティー Monoclonal
クローン 1H9
アイソタイプ
(免疫動物)		 Mouse IgG1
使用法 WB　2-10 µg/mL  
IP*　reported.  （PMID: 2472999）
IH　2-10 µg/mL  
交差性 [Gene ID]

Human[495], Mouse[11944], Rat[24216], Bovine[521163]
性状 PBS/50% glycerol, pH 7.2
保存温度 -20°C
標識 Unlabeled
メーカー MBL
別称 ATP4A, ATP6A
背景 Hydrogen-potassium adenosine triphosphatase (H+, K+-ATPase) belongs to a family of P-type cation-transporting ATPases that also includes Ca2+-ATPase and Na+, K+-ATPase. H+, K+-ATPase is responsible for acid secretion into the stomach and catalyzes electroneutral exchange of cytoplasmic hydrogen ions and external potassium ions coupled with ATP hydrolysis. The enzyme is found in gastric parietal cells where it is located in cytoplasmic vesicles or apical plasma membranes of the secretory canaliculus. H+, K+-ATPase is a heterodimer consisting of a high molecular weight catalytic a subunit and a smaller but heavily glycosylated b subunit. The a subunit of H+, K+-ATPase has 10 transmembrane domains and contains sites for ATP binding and its acylphosphorylation, binding sites of proton pump inhibitors, and sites responsible for ion recognition.
関連製品 D031-3H Anti-Proton Pump (H,K-ATPase α subunit) mAb
D032-3 Anti-Proton Pump (H,K-ATPase β subunit) mAb
D032-3H Anti-Proton Pump (H,K-ATPase β subunit) mAb
製品カテゴリー ツール:その他
データ

使用文献

Western Blotting

  1. Chandra M et al. A role for the Ca2+ channel TRPML1 in gastric acid secretion, based on analysis of knockout mice. Gasteroenterology 140, 857-67 (2011)(PMID:21111738)
  2. Fujii T et al. Non-morphogenic effect of Sonic Hedgehog on gastric H+,K+-ATPase activity. Biochem Biophys Res Commun. 518, 605-609 (2019)(PMID:31445709)

Immunohistochemistry

  1. Kawamura YI et al. Introduction of Sd(a) carbohydrate antigen in gastrointestinal cancer cells eliminates selectin ligands and inhibits metastasis. Cancer Res. 65, 6220-7 (2005)(PMID:16024623)
  2. Mimata A et al. Loss of E-cadherin in mouse gastric epithelial cells induces signet ring-like cells, a possible precursor lesion of diffuse gastric cancer. Cancer Sci. 102, 942-50 (2011)(PMID:21276134)
  3. Becker V et al. New aspects in the pathomechanism and diagnosis of the laryngopharyngeal reflux-clinical impact of laryngeal proton pumps and pharyngeal pH metry in extraesophageal gastroesophageal reflux disease. World J. Gasteroenterol. 21, 982-7 (2015)(PMID:25624734)
  4. Mitsuishi T et al. Clinicopathological characteristics of duodenal epithelial neoplasms: Focus on tumors with a gastric mucin phenotype (pyloric gland-type tumors). PLoS One. 12, e0174985 (2017)
     (PMID:28376132)
  5. Suzuki K et al. Deficiency of Stomach-Type Claudin-18 in Mice Induces Gastric Tumor Formation Independent of H pylori Infection. Cell Mol Gastroenterol Hepatol. 8, 119-142 (2019)(PMID:30910700)
  6. Ogawa T et al. CHAC1 overexpression in human gastric parietal cells with Helicobacter pylori infection in the secretory canaliculi. Helicobacter. 24, e12598 (2019)(PMID:31111570)
  7. Fujii T et al. Non-morphogenic effect of Sonic Hedgehog on gastric H+,K+-ATPase activity. Biochem Biophys Res Commun. 518, 605-609 (2019)(PMID:31445709)
参考文献
  1. Mori Y et al. Parietal cell autoantigens involved in neonatal thymectomy-induced murine autoimmune gastritis. Studies using monoclonal autoantibodies. Gasteroenterology 97, 364-75 (1989)（PMID: 2472999）

※在庫につきましては2024年04月19日 17時00分時点における在庫数を表示してあります。
※価格の赤色表示につきましては、キャンペーン価格の表示となっております。
※このサイトからは直接注文はできません。ご注意ください。
※使用法の表記について：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation,
FCM: Flow Cytometry NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation,
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation,
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
※使用法・交差性の表記について：
*: 論文で報告されております（MBLでは未確認）。詳しくはデータシートをご覧ください。
**: 導入元からの情報です（MBLでは評価中もしくは未確認）。
※保存温度の表記について： RT: 室温
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