トップ > 製品検索結果一覧 > Code No. M114-3 Anti-SUMO-2/3 mAb

Code No.　M114-3

Anti-SUMO-2/3 mAb

価格（税別）

¥52,800

在庫

10以上

（2024/04/20 00:05時点）

包装

100 µL (1 mg/mL)

This antibody reacts with SUMO-2/3 (15 kDa) but not react with SUMO-1 (15~17 kDa) on Western blotting.
データ

  • Western Blotting

  • Immunocytochemistry
クローナリティー Monoclonal クローン 1E7
アイソタイプ
（免疫動物） 		Mouse IgG2b
使用法
WB
2 µg/mL  
IC
5 µg/mL  
ChIP*
reported.  （PMID: 26259101）
Other(PLA)
reported.  （PMID: 28600321）
免疫原(抗原) Recombinant Human full-length SUMO-3
交差性
[Gene ID]

Human[6613/6612], Mouse[170930/20610], Rat[690244/499417]
性状 1 mg/mL in PBS/50% glycerol, pH 7.2
保存温度 -20°C 標識 Unlabeled メーカー MBL
別称 SUMO2, SUMO3, HSMT3, SMT3A, SMT3B, Smt3A, Smt3B, SMT3H1, SMT3H2
背景 Sumoylation, the covalent attachment of a small ubiquitin-like modifier (SUMO) peptide to lysine residues of targeted substrate, has recently emerged as an important mechanism in transcriptional control. In humans and mice, the SUMO family consists of three members, SUMO-1, -2, and -3. SUMO-2 (SMT3A, Sentrin-3) and SUMO-3 (SMT3B, Sentrin-2) are similar (~95% identical), but less closely related to SUMO-1 (~50% identical). Whereas many proteins modified by SUMO-1 were identified, many as yet unidentified proteins are modified by SUMO-2/3 after exposure of cells to various stress stimuli.
関連製品 M113-3 Anti-SUMO-1 mAb
D058-3 Anti-Multi Ubiquitin mAb
MK-12-3 Anti-Ubiquitin mAb
使用文献
 使用文献募集中！本製品を使って論文を発表されましたら是非お知らせください。

Western Blotting

  1. Klein UR et al. RanBP2 and SENP3 function in a mitotic SUMO2/3 conjugation-deconjugation cycle on Borealin. Mol. Biol. Cell 20, 410-8 (2009)(PMID:18946085)
  2. Du JX et al. A small ubiquitin-related modifier-interacting motif functions as the transcriptional activation domain of Krüppel-like factor 4. J biol. Chem. 285, 28298-308 (2010)(PMID:20584900)
  3. Fernández-Miranda G et al. SUMOylation modulates the function of Aurora-B kinase. J Cell Sci. 123, 2823-33 (2010)(PMID:20663916)
  4. Finkbeiner E et al. The SUMO system controls nucleolar partitioning of a novel mammalian ribosome biogenesis complex. EMBO J. 30, 1067-78 (2011)(PMID:21326211)
  5. Fanis P et al. Five friends of methylated chromatin target of protein-arginine-methyltransferase[prmt]-1 (chtop), a complex linking arginine methylation to desumoylation. Mol Cell Proteomics 11, 1263-73 (2012)(PMID:23122649)
  6. Psakhye I, Jentsch S. Protein group modification and synergy in the SUMO pathway as exemplified in DNA repair. Cell 151, 807-20 (2012)(PMID:23122649)
  7. Guo C et al. SENP3-mediated deSUMOylation of dynamin-related protein 1 promotes cell death following ischaemia. EMBO J. 32, 1514-28 (2013)(PMID:23524851)
  8. Berndt A et al. In vivo characterization of the properties of SUMO1-specific monobodies. Biochem J. 456, 385-95 (2013)(PMID:24040933)
  9. Paakinaho V et al. Electrophilic lipid mediator 15-deoxy-Δ12,14-prostaglandin j2 modifies glucocorticoid signaling via receptor SUMOylation. Mol Cell Biol. 34, 202-13 (2014)(PMID:24980431)
  10. Sutinen P et al. Nuclear mobility and activity of FOXA1 with androgen receptor are regulated by SUMOylation. Mol Endocrinol. 28, 1719-28 (2014)(PMID:25127374)
  11. Niskanen EA et al. Global SUMOylation on active chromatin is an acute heat stress response restricting transcription. Genome Biol. 16, 153 (2015)(PMID:26259101)
  12. Yan Y et al. SUMOylation of AMPKα1 by PIAS4 specifically regulates mTORC1 signalling. Nat Commun. 6, 8979 (2015)(PMID:26616021)
  13. Lecona E et al. USP7 is a SUMO deubiquitinase essential for DNA replication. Nat Struct Mol Biol. 23, 270-7 (2016)(PMID:26950370)
  14. Du L et al. Role of SUMO activating enzyme in cancer stem cell maintenance and self-renewal. Nat Commun. 7, 12326 (2016)(PMID:27465491)
  15. Ambaye N et al. Streptonigrin Inhibits SENP1 and Reduces the Protein Level of Hypoxia-Inducible Factor 1α (HIF1α) in Cells. Biochemistry 57, 1807-1813 (2018)(PMID:29481054)
  16. Eifler K et al. SUMO targets the APC/C to regulate transition from metaphase to anaphase. Nat Commun. 9, 1119 (2018)(PMID:29549242)
  17. Li YJ et al. Regulation of miR-34b/c-targeted gene expression program by SUMOylation. Nucleic Acids Res. 46, 7108-7123 (2018)(PMID:29893976)
  18. Zhang FP et al. Lack of androgen receptor SUMOylation results in male infertility due to epididymal dysfunction. Nat Commun. 10, 777 (2019) (PMID:30770815)

ChIP

  1. Niskanen EA et al. Global SUMOylation on active chromatin is an acute heat stress response restricting transcription. Genome Biol. 16, 153 (2015)(PMID:26259101)

Immunocytochemistry

  1. Lecona E et al. USP7 is a SUMO deubiquitinase essential for DNA replication. Nat Struct Mol Biol. 23, 270-7 (2016)(PMID:26950370)

Other(PLA)

  1. Gilistro E et al. Importin-β and CRM1 control a RANBP2 spatiotemporal switch essential for mitotic kinetochore function. J Cell Sci. 130, 2564-2578 (2017)(PMID:28600321)
製品
カテゴリー
分野
ユビキチンプロテアソーム系
この製品に関するお問い合わせをする

※在庫につきましては2024年04月20日 00時05分時点における在庫数を表示してあります。
※価格の赤色表示につきましては、キャンペーン価格の表示となっております。
※このサイトからは直接注文はできません。ご注意ください。
※使用法の表記について：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation,
FCM: Flow Cytometry NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation,
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
※使用法・交差性の表記について：
*: 論文で報告されております（MBLでは未確認）。詳しくはデータシートをご覧ください。
**: 導入元からの情報です（MBLでは評価中もしくは未確認）。
※保存温度の表記について： RT: 室温
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