Anti-5-methylcytidine (m⁵C) mAb

価格（税別）

¥66,000

在庫

1-9

（2024/04/19 00:05時点）

包装

100 µL (1 mg/mL)

使用文献

データシート

SDS

Anti-5-methylcytidine (m⁵C) mAb (Clone: FMC-9) reacts with 5-methylcytidine (m⁵C or m5Cyd), 5-methyl-2’-deoxycytidine (m5dC or m5dCyd) and 5-methylcytosine (5mC, 5-mC or m5Cyt).

データ	Immunoprecipitation MeDIP was performed using mouse genomic DNA fragments containing synthesized DNAs. IP'd samples were analyzed by real-time qPCR using primers specific to synthetic DNA spike-in controls or Loci 1-3. Immunocytochemistry Immunohistochemistry Immunohistochemistry Dot Blotting
クローナリティー	Monoclonal	クローン	FMC-9
アイソタイプ（免疫動物）	Mouse IgG2aλ
使用法	IP Can be used. IC 5 µg/mL (for cytoplasmic staining), 1 µg/mL (for nuclear staining, HCl-treated) IH 10 µg/mL (For nuclear staining, heat treatment or HCl treatment is necessary.) ELISA Inhibition ELISA (Info. from the licenser. Not tested by MBL.) DB Can be used. Other(Immuno-Nothern blotting) Can be used. （PMID: 26606401）
免疫原(抗原)	KLH-conjugated 5-methylcytidine (KLH-m⁵C)
性状	1 mg/mL in PBS/50% glycerol, pH 7.2
保存温度	-20°C	標識	Unlabeled	メーカー	MBL
別称	m5Cyd
背景	5-Methylcytosine (5mC), a modified nucleobase derived from cytosine, is present in both DNA and RNA molecules. 5mC in DNA is an essential epigenetic mark associated with gene silencing and genome stability during embryonic development. 5mC in ribonucleoside, called 5-methylcytidine (m⁵C), is involved in structural stability, aminoacylation and codon recognition of tRNA. In rRNA, m⁵C affects translational fidelity and tRNA recognition. m⁵C-modified nucleobase also have been identified in mRNA and other non-coding RNA but the biological functions are still unclear. m⁵C is detected not only in tissues and cells but also in urine. It is reported that the levels of m⁵C change in urine or tissues from patients with various diseases such as bladder, breast and colon cancers.
関連製品	D345-3 Anti-1-methyladenosine (m¹A) mAb PM077 Anti-5-hydroxymethylcytosine (5hmC) pAb MI-11-3 Anti-Bromodeoxyuridine mAb M076-3 Mouse IgG2a (isotype control) PM085 Anti-ZBTB24 pAb PM086 Anti-ZBTB24 (Human) pAb D347-3 Anti-Pseudouridine mAb M227-3 Anti-Digoxigenin (DIG) mAb M218-3 Anti-5-hydroxymethylcytosine (5hmC) mAb M228-3 Anti-FITC mAb RN019M Anti-2,2,7-trimethylguanosine (m₃G/TMG) mAb RN016M Anti-7-methylguanosine (m⁷G)-Cap mAb RN017M Anti-7-methylguanosine (m⁷G) mAb D289-3 Anti-Np95 (Uhrf1) (Mouse) mAb M202-3 Anti-Np95 (Uhrf1) mAb RN127PW Anti-NSUN2 (Human) pAb PM098 Anti-Inosine pAb
使用文献	使用文献募集中！本製品を使って論文を発表されましたら是非お知らせください。 Dot Blotting Garvilles RG et al. Dual Functions of the RFTS Domain of Dnmt1 in Replication-Coupled DNA Methylation and in Protection of the Genome from Aberrant Methylation. PLoS One 10, e0137509 (2015)(PMID:26383849) Immunofluorescence Totsuka T et al. Production of mouse androgenetic embryos using spindle perturbation. Sci Rep. 10, 6556 (2020)(PMID:32300113) Other(Immuno-Nothern blotting) Mishima E et al. Immuno-Northern Blotting: Detection of RNA Modifications by Using Antibodies against Modified Nucleosides. PLoS One 10, e0143756 (2015)(PMID:26606401)
参考文献	Seki Y et al. Extensive and orderly reprogramming of genome-wide chromatin modifications associated with specification and early development of germ cells in mice. Dev Biol. 278, 440-58 (2005)（PMID: 15680362） Sakai Y et al. Expression of DNA methyltransferase (Dnmt1) in testicular germ cells during development of mouse embryo. Cell Struct Funct. 26, 685-91 (2001)（PMID: 11942627） Mizugaki M et al. Preparation of a monoclonal antibody specific for 5-methyl-2'-deoxycytidine and its application for the detection of DNA methylation levels in human peripheral blood cells. Biol Pharm Bull. 19, 1537-40 (1996)（PMID: 8996634）
製品カテゴリー	分野 RNA-RNPネットワークエピジェネティクス

※在庫につきましては2024年04月19日 00時05分時点における在庫数を表示してあります。
※価格の赤色表示につきましては、キャンペーン価格の表示となっております。
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※使用法の表記について：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation,
FCM: Flow Cytometry NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation,
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
※使用法・交差性の表記について：
*: 論文で報告されております（MBLでは未確認）。詳しくはデータシートをご覧ください。
**: 導入元からの情報です（MBLでは評価中もしくは未確認）。
※保存温度の表記について： RT: 室温
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