Anti-Mincle (Mouse) mAb

価格（税別）

¥52,800

在庫

10以上

（2024/04/20 17:00時点）

包装

100 µL (1 mg/mL)

使用文献

データシート

SDS

データ	Immunoprecipitation Flow Cytometry
クローナリティー	Monoclonal	クローン	4A9
アイソタイプ（免疫動物）	Rat IgG1 κ
使用法	WB 1-5 µg/mL IP 5-10 µg/200 µL cell extract from 1 x 10⁶ cells FCM 5-10 µg/mL (final concentration) Function 1-10 µg/mL (final concentration) （PMID: 18776906）
免疫原(抗原)	Mouse Mincle (full length) transfected RBL-2H3 cells
交差性 [Gene ID]	Mouse[56619]
性状	1 mg/mL in PBS/50% glycerol, pH 7.2
保存温度	-20°C	標識	Unlabeled	メーカー	MBL
別称	Clec4e, C-type lectin domain family 4, member e, C86253, Clecsf9
背景	Macrophage-inducible C-type lectin (Mincle, also called as Clec4e and Clecsf9), a type II transmembrane C-type lectin receptor, is expressed mainly in macrophages. Mincle is selectively associated with the Fc receptor common g-chain which contains immunoreceptor tyrosine-based activation motif (ITAM) and activates macrophages to produce inflammatory cytokines and chemokines. Mincle expressing cells are activated by spliceosome associated protein 130 (SAP130) which is a component of small nuclear ribonucleoprotein released from dead cells. Recently, Mincle is reported to recognize Mycobacterium tuberculosis as well as pathogenic fungus Malassezia species. Mincle is demonstrated to be an essential receptor for a mycobacterial glycolipid, trehalose-6,6’-dimycolate (TDM). The cytokine/chemokine production of macrophages which Malassezia or M.tuberculosis induces in Mincle-/- mice is significantly impaired. Mincle is considered to play a crucial role in immune responses to pathogens.
関連製品	D266-3 Anti-Mincle (Mouse) mAb D292-3M2 Anti-Mincle (Mouse) mAb (Functional Grade) D266-3M2 Anti-Mincle (Mouse) mAb (Functional Grade) D266-6 Anti-Mincle (Mouse) mAb-Biotin D360-3 Anti-Mincle (Human) mAb (Functional Grade) M191-3 Anti-FcεR1γ (FcRγ) (Mouse) mAb M191-A48 Anti-FcεR1γ (FcRγ) (Mouse) mAb-Alexa Fluor™ 488 M191-A59 Anti-FcεR1γ (FcRγ) (Mouse) mAb-Alexa Fluor™ 594 M191-A64 Anti-FcεR1γ (FcRγ) (Mouse) mAb-Alexa Fluor™ 647
使用文献	使用文献募集中！本製品を使って論文を発表されましたら是非お知らせください。 Western Blotting Mohanraj M et al. The Mycobacterial Adjuvant Analogue TDB Attenuates Neuroinflammation via Mincle-Independent PLC-γ1/PKC/ERK Signaling and Microglial Polarization. Mol Neurobiol. (2018) In press.(PMID:29876879) Tan RZ et al. Curcumin relieved cisplatin-induced kidney inflammation through inhibiting Mincle-maintained M1 macrophage phenotype. Phytomedicine 52, 284-294 (2019)(PMID:30599909) Flow Cytometry Miyake Y et al. C-type Lectin MCL Is an FcR?A-Coupled Receptor that Mediates the Adjuvanticity of Mycobacterial Cord Factor. Immunity. 38, 1050-62 (2013)(PMID:23602766) Toyonaga K et al. Characterization of the receptors for mycobacterial cord factor in Guinea pig. PLoS One 9, e88747 (2014)(PMID:24533147) Mohanraj M et al. The Mycobacterial Adjuvant Analogue TDB Attenuates Neuroinflammation via Mincle-Independent PLC-γ1/PKC/ERK Signaling and Microglial Polarization. Mol Neurobiol. (2018) In press.(PMID:29876879) Fites JS et al. An unappreciated role for neutrophil-DC hybrids in immunity to invasive fungal infections. PLoS Pathog. 14, e1007073 (2018)(PMID:29782541) Function Yamasaki S et al. Mincle is an ITAM-coupled activating receptor that senses damaged cells. Nat Immunol. 9, 1179-88 (2008)(PMID:18776906)
製品カテゴリー	分野免疫

※在庫につきましては2024年04月20日 17時00分時点における在庫数を表示してあります。
※価格の赤色表示につきましては、キャンペーン価格の表示となっております。
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※使用法の表記について：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation,
FCM: Flow Cytometry NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation,
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
※使用法・交差性の表記について：
*: 論文で報告されております（MBLでは未確認）。詳しくはデータシートをご覧ください。
**: 導入元からの情報です（MBLでは評価中もしくは未確認）。
※保存温度の表記について： RT: 室温
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