トップ > 製品検索結果一覧 > Code No. D076-3 Anti-Phosphorylated Vimentin (Ser55) mAb

Code No.　D076-3

Anti-Phosphorylated Vimentin (Ser55) mAb

価格（税別）

¥52,800

在庫

10以上

（2024/04/20 00:05時点）

包装

100 µL (1 mg/mL)

データ

  • Western Blotting

  • Immunocytochemistry
クローナリティー Monoclonal クローン 4A4
アイソタイプ
（免疫動物） 		Mouse IgG2b
使用法
WB
1-5 µg/mL  
IC
1 µg/mL  
IH*
reported.  （PMID: 15175243 / 15634780）
ELISA*
reported.  （PMID: 7983050）
免疫原(抗原) Synthetic MPV55 phophopeptide corresponding to Mouse phophorylated vimentin Ser55 (SLYSS-phosphoS55-PGGAYC-KLH)
交差性
[Gene ID]

Human[7431], Mouse[22352], Rat[81818]
性状 1 mg/mL in PBS/50% glycerol, pH 7.2
保存温度 -20°C 標識 Unlabeled メーカー MBL
別称 Vim, vimentin
背景 Vimentin is an intermediate filament protein distributed widely in the cytoplasm and is phosphorylated by several protein kinase in vitro. Ser55 residues on vimentin were reported to be one of the phosphorylation sites of vimentin at metaphase and were the phosphorylation sites for cdc2 kinase but not for cAMP-dependent protein kinase, protein kinase C, and Ca2+-calmodulin-dependent protein kinase II in vitro. Immunofluorescence and immunoelectron microscopy showed that vimentin Ser55 residues distributed in the entire cytoplasmic vimentin filament system are phosphorylated when the cells enter mitosis and de-phosphorylated in cytokinesis. The use of this antibody that specifically reacts with the phosphorylation site of vimentin Ser55 by cdc2 kinase enables estimation of a particular cdc2 kinase function.
関連製品 D093-3 Anti-Phosphorylated Vimentin (Ser71) mAb
D095-3 Anti-Phosphorylated Vimentin (Ser82) mAb
使用文献
 使用文献募集中！本製品を使って論文を発表されましたら是非お知らせください。

Western Blotting

  1. Tsujimura K et al. Visualization and function of vimentin phosphorylation by cdc2 kinase during mitosis. J Biol Chem. 269, 31097-106 (1994)(PMID:7983050)
  2. Fourest-Lieuvin A et al. Microtubule regulation in mitosis: tubulin phosphorylation by the cyclin-dependent kinase Cdk1. Mol Biol Cell. 17, 1041-50 (2006)(PMID:16371510)
  3. Tatsumi Y et al. Deregulation of Cdt1 induces chromosomal damage without rereplication and leads to chromosomal instability. J Cell Sci. 119, 3128-40 (2006)(PMID:16835273)
  4. Chang IA et al. Vimentin phosphorylation by Cdc2 in Schwann cell controls axon growth via β1-integrin activation. FASEB J. 26, 2401-13 (2012)(PMID:22371530)
  5. Yokoyama T et al. Identification of candidate molecular targets of the novel antineoplastic antimitotic NP-10. Sci Rep. 9, 16825 (2019)(PMID:31727981)

Immunocytochemistry

  1. Tsujimura K et al. Visualization and function of vimentin phosphorylation by cdc2 kinase during mitosis. J Biol Chem. 269, 31097-106 (1994)(PMID:7983050)
  2. Tsai J W et al. LIS1 RNA interference blocks neural stem cell division, morphogenesis, and motility at multiple stages. J Cell Biol. 170, 935-45 (2005)(PMID:16144905)
  3. Chang IA et al. Vimentin phosphorylation by Cdc2 in Schwann cell controls axon growth via β1-integrin activation. FASEB J. 26, 2401-13 (2012)(PMID:22371530)

Immunohistochemistry

  1. Miyata T et al. Asymmetric production of surface-dividing and non-surface-dividing cortical progenitor cells. Development. 131, 3133-45 (2004)(PMID:15175243)
  2. Englund C et al. Pax6, Tbr2, and Tbr1 are expressed sequentially by radial glia, intermediate progenitor cells, and postmitotic neurons in developing neocortex. J Neurosci. 25, 247-51 (2005)(PMID:15634788)
  3. Minobe S et al. Rac is involved in the interkinetic nuclear migration of cortical progenitor cells. Neurosci Res. 63, 294-301 (2009)(PMID:19367791)
  4. Shitamukai A et al. Oblique radial glial divisions in the developing mouse neocortex induce self-renewing progenitors outside the germinal zone that resemble primate outer subventricular zone progenitors. J Neurosci. 31, 3683-95 (2011)(PMID:21389223)
  5. Chang IA et al. Vimentin phosphorylation by Cdc2 in Schwann cell controls axon growth via β1-integrin activation. FASEB J. 26, 2401-13 (2012)(PMID:22371530)
  6. Martínez-Cerdeño V et al. N-Myc and GCN5 regulate significantly overlapping transcriptional programs in neural stem cells. PLoS One 7, e39456 (2012)(PMID:22745758)
  7. Malik S et al. Neurogenesis continues in the third trimester of pregnancy and is suppressed by premature birth. J Neurosci. 33, 411-23 (2013)(PMID:23303921)
  8. Martínez‐Cerdeño V et al. Evolutionary origin of Tbr2-expressing precursor cells and the subventricular zone in the developing cortex. J Comp Neurol. 524, 433-47 (2016)(PMID:26267763)
  9. Delgado RN, Lim DA. Embryonic Nkx2.1-expressing neural precursor cells contribute to the regional heterogeneity of adult V-SVZ neural stem cells. Dev Biol. 407, 265-74 (2015)(PMID:26387477)
  10. Wang L et al. Hedgehog signaling promotes basal progenitor expansion and the growth and folding of the neocortex. Nat Neurosci. 19, 888-96 (2016)(PMID:27214567)
  11. Garbriel E et al. Recent Zika Virus Isolates Induce Premature Differentiation of Neural Progenitors in Human Brain Organoids. Cell Stem Cell 20, 397-406.e5 (2017)(PMID:28132835)
  12. Bosshard M et al. Impaired oxidative stress response characterizes HUWE1-promoted X-linked intellectual disability. Sci Rep. 7,15050 (2017)(PMID:29118367)
  13. Zhou X et al. Cellular and molecular properties of neural progenitors in the developing mammalian hypothalamus. Nat Commun. 11, 4063 (2020)(PMID:32792525)
製品
カテゴリー
分野
シグナル伝達
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※在庫につきましては2024年04月20日 00時05分時点における在庫数を表示してあります。
※価格の赤色表示につきましては、キャンペーン価格の表示となっております。
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※使用法の表記について：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation,
FCM: Flow Cytometry NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation,
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
※使用法・交差性の表記について：
*: 論文で報告されております（MBLでは未確認）。詳しくはデータシートをご覧ください。
**: 導入元からの情報です（MBLでは評価中もしくは未確認）。
※保存温度の表記について： RT: 室温
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