トップ > 製品検索結果一覧 > Code No. RN025P Anti-PCBP2 pAb

Code No. RN025P

Anti-PCBP2 pAb

価格(税別)

¥60,000

在庫

10以上

(2019/10/14 17:00時点)

包装

200 µg/200 µL

データ
  • Western Blotting

  • Immunoprecipitation

  • RNP Immunoprecipitation

クローナリティー Polyclonal クローン Polyclonal
アイソタイプ
(免疫動物)
Rabbit Ig (aff.)
使用法
WB
1 µg/mL  
IP
5 µg/500 µL of cell extract from 5 x 106 cells  
IC*
reported.  (PMID: 26990993
RIP
15 µg/500 µL of cell extract from 1.5 x 107 cells  
Other(Immunodepletion)
reported.  (PMID: 22438951
Other(eCLIP)
reported.  (PMID: 27018577
免疫原(抗原) KLH conjugated synthetic peptide, corresponding to internal region of human PCBP2
交差性
[Gene ID]

Human[5094](22438951), Mouse[18521], Rat[363005]

性状 1 mg/mL in PBS/50% glycerol, pH 7.2
保存温度 -20°C 標識 Unlabeled メーカー MBL
別称 poly(rC) binding protein 2, HNRPE2, hnRNP-E2
背景 Poly(rC)-binding proteins (PCBPs) are cellular RNA-binding proteins, characterized by their triple KH structure and by their poly(C)-binding specificity. The 3 KH domains are not only involved in nucleic acid binding but also mediate protein-protein interactions within the RNP complexes. It binds specifically to polypyrimidine tracts within the 3' UTR of target mRNAs through KH domains. PCBPs are expressed as 4 isoforms in cells. PCBP1 and PCBP2 are predominantly expressed in the nucleus and can shuttle from the nucleus to the cytoplasm, while PCBP3 and PCBP4 are expressed to a less extent in the cytoplasm. The most characterized gene regulated by PCBP1 and PCBP2 is the a-globin gene, and the accumulated α-globin mRNA is known to account for approximately 95% of total cellular mRNA during terminal erythroid differentiation. Specific binding of PCBPs to 3′ UTR of mRNA is associated with the stabilization of a-globin mRNA. Microarray analysis of human mRNAs present in a specific subset of PCBP2-RNP complexes also showed a potential involvement of PCBP2 in transcription factor expression via association with their mRNA; further, the enrichment of PCBP2 mRNA in PCBP2-RNP complexes suggested that PCBP2 expression may be subject to posttranscriptional autoregulation.
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使用文献
使用文献募集中!本製品を使って論文を発表されましたら是非お知らせください。

Western Blotting

  1. Neff AT et al. Global analysis reveals multiple pathways for unique regulation of mRNA decay in induced pluripotent stem cells. Genome Res. 1457-67 (2012)(PMID:22534399)

Immunocytochemistry

  1. Sundararaman B et al. Resources for the Comprehensive Discovery of Functional RNA Elements. Mol Cell. 61, 903-13 (2016)(PMID:26990993)
  2. Markmiller S et al. Context-Dependent and Disease-Specific Diversity in Protein Interactions within Stress Granules. Cell. 172, 590-604.e13 (2018)(PMID:29373831)

RNP Immunoprecipitation

  1. Palusa S et al. The 3' untranslated region of the rabies virus glycoprotein mRNA specifically interacts with cellular PCBP2 protein and promotes transcript stability. PLoS One. 7, e33561 (2012)(PMID:22438951)

Other(Immunodepletion)

  1. Palusa S et al. The 3' untranslated region of the rabies virus glycoprotein mRNA specifically interacts with cellular PCBP2 protein and promotes transcript stability. PLoS One. 7, e33561 (2012)(PMID:22438951)

Other(eCLIP)

  1. Sundararaman B et al. Resources for the Comprehensive Discovery of Functional RNA Elements. Mol Cell. 61, 903-13 (2016)(PMID:26990993)
  2. Van Nostrand EL et al. Robust transcriptome-wide discovery of RNA-binding protein binding sites with enhanced CLIP (eCLIP). Nat Methods. 13, 508-14 (2016)(PMID:27018577)
製品
カテゴリー
分野
RNA-RNPネットワーク
ブランド
使用文献・データ募集プレゼント対象製品
RiboCluster Profiler™
この製品に関するお問い合わせをする

※在庫につきましては2019年10月14日 17時00分時点における在庫数を表示してあります。
※価格の赤色表示につきましては、キャンペーン価格の表示となっております。
※このサイトからは直接注文はできません。ご注意ください。
※使用法の表記について:
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation,
FCM: Flow Cytometry NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation,
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
※使用法・交差性の表記について:
*: 論文で報告されております(MBLでは未確認)。詳しくはデータシートをご覧ください。
**: 導入元からの情報です(MBLでは評価中もしくは未確認)。
※保存温度の表記について: RT: 室温
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