トップ > 製品検索結果一覧 > Code No. M048-3 Anti-GFP (Green Fluorescent Protein) mAb

Code No. M048-3

Anti-GFP (Green Fluorescent Protein) mAb

価格(税別)

¥48,000

在庫

10以上

(2019/12/08 17:00時点)

包装

100 µg/100 µL

データ
  • Western Blotting

  • Immunoprecipitation

  • Immunocytochemistry

  • Immunohistochemistry

  • Immunohistochemistry

クローナリティー Monoclonal クローン 1E4
アイソタイプ
(免疫動物)
Mouse IgG2b
使用法
WB
1 µg/mL  
IP
5 µg/sample  
IC
2 µg/mL  
IH
10 µg/mL  
IF*
reported.  (PMID: 26581759
免疫原(抗原) Recombinant full-length GFP (246 a.a.)
性状 1 mg/mL in PBS/50% glycerol, pH 7.2
保存温度 -20°C 標識 Unlabeled メーカー MBL
背景 Green Fluorescent Protein (GFP) tagging provide an excellent means for monitoring gene expression and protein localization in living cells, so that it is widely accepted by molecular and cell biological research. Monoclonal anti-GFP antibody can detect GFP and its variants on Western blotting, Immunoprecipitation and Immunocytochemistry.
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D153-3 Anti-GFP (Green Fluorescent Protein) mAb
D153-8 Anti-GFP (Green Fluorescent Protein) mAb-Agarose
D153-10 Anti-GFP (Green Fluorescent Protein) mAb-Magnetic Agarose
D153-A48 Anti-GFP (Green Fluorescent Protein) mAb-Alexa Fluor® 488
D153-A59 Anti-GFP (Green Fluorescent Protein) mAb-Alexa Fluor® 594
D153-A64 Anti-GFP (Green Fluorescent Protein) mAb-Alexa Fluor® 647
D153-11 Anti-GFP (Green Fluorescent Protein) mAb-Magnetic Beads
PM073 Anti-Renilla GFP pAb
使用文献
使用文献募集中!本製品を使って論文を発表されましたら是非お知らせください。

Western Blotting

  1. Abe Y et al. Mammalian Gup1, a homolog of Saccharomyces cerevisiae glycerol uptake/transporter 1, acts as a negative regulator for N-terminal palmitoylation of Sonic hedgehog. FEBS J. 275, 318-31 (2008)(PMID:18081866)
  2. Komuro A et al. Diffuse-type gastric carcinoma: progression, angiogenesis, and transforming growth factor beta signaling. J Natl Cancer Inst. 101, 592-604 (2009)(PMID:19351925)
  3. Takahata M et al. Ro52 functionally interacts with IgG1 and regulates its quality control via the ERAD system. Mol Immunol. 45, 2045-54 (2008)(PMID:18022694)
  4. Masuda M et al. The tumor suppressor protein TSLC1 is involved in cell-cell adhesion. J Biol Chem. 277, 31014-9 (2002)(PMID:12050160)
  5. Nakamichi I et al. Formation of Mallory body-like inclusions and cell death induced by deregulated expression of keratin 18. Mol Biol Cell 13, 3441-51 (2002)(PMID:12388748)
  6. Song Z et al. Direct interaction between survivin and Smac/DIABLO is essential for the anti-apoptotic activity of survivin during taxol-induced apoptosis. J Biol Chem. 278, 23130-40 (2003)(PMID:12660240)
  7. de Graaf K et al. Characterization of cyclin L2, a novel cyclin with an arginine/serine-rich domain: phosphorylation by DYRK1A and colocalization with splicing factors. J Biol Chem. 279, 4612-24 (2004)(PMID:14623875)
  8. Iwai S et al. A novel actin-bundling kinesin-related protein from Dictyostelium discoideum. J Biol Chem. 279, 4696-704 (2004)(PMID:14623897)
  9. Lozupone F et al. Identification and relevance of the CD95-binding domain in the N-terminal region of ezrin. J Biol Chem. 279, 9199-207 (2004)(PMID:14676203)
  10. Pottekat A, Menon AK. Subcellular localization and targeting of N-acetylglucosaminyl phosphatidylinositol de-N-acetylase, the second enzyme in the glycosylphosphatidylinositol biosynthetic pathway. J Biol Chem. 279, 15743-51 (2004)(PMID:14742432)
  11. Hisatsune C et al. Regulation of TRPC6 channel activity by tyrosine phosphorylation. J Biol Chem. 279, 18887-94 (2004)(PMID:14761972)
  12. Kontrogianni-Konstantopoulos A et al. Obscurin regulates the organization of myosin into A bands. Am J Physiol Cell Physiol. 287, C209-17 (2004)(PMID:15013951)
  13. Hanamoto T et al. Identification of protein kinase A catalytic subunit beta as a novel binding partner of p73 and regulation of p73 function. J Biol Chem. 280, 16665-75 (2005)(PMID:15723830)
  14. Reversi A et al. Effects of cholesterol manipulation on the signaling of the human oxytocin receptor. Am J Physiol Regul Integr Comp Physiol. 291, R861-9 (2006)(PMID:16966388)
  15. Takeda K et al. Apoptosis signal-regulating kinase (ASK) 2 functions as a mitogen-activated protein kinase kinase kinase in a heteromeric complex with ASK1. J Biol Chem. 282, 7522-31 (2007)(PMID:17210579)
  16. Lebel-Haziv Y et al. Breast cancer: coordinated regulation of CCL2 secretion by intracellular glycosaminoglycans and chemokine motifs. Neoplasia 16, 723-40 (2014)(PMID:25246273)
  17. Zhang B et al. GSK3β-Dzip1-Rab8 cascade regulates ciliogenesis after mitosis. PLoS Biol. 13, e1002129 (2015)(PMID:25860027)
  18. Li Z et al. Destabilization of linker histone H1.2 is essential for ATM activation and DNA damage repair. Cell Res. 28, 756-770 (2018)(PMID:29844578)
  19. Tsukune N et al. Forced expression of mouse progerin attenuates the osteoblast differentiation interrupting β-catenin signal pathway in vitro. Cell Tissue Res. 375, 655-664 (2019)(PMID:30284086)

Immunoprecipitation

  1. Banerjee S et al. A coordinated local translational control point at the synapse involving relief from silencing and MOV10 degradation. Neuron. 64, 871-84 (2009)(PMID:20064393)
  2. Asada N, Sanada K. LKB1-mediated spatial control of GSK3beta and adenomatous polyposis coli contributes to centrosomal forward movement and neuronal migration in the developing neocortex. J Neurosci. 30, 8852-65 (2010)(PMID:20592207)
  3. Kiriyama M et al. Interaction of FLASH with arsenite resistance protein 2 is involved in cell cycle progression at S phase. Mol Cell Biol. 29, 4729-41 (2009)(PMID:19546234)
  4. Kojima K et al. Associations between PIWI proteins and TDRD1/MTR-1 are critical for integrated subcellular localization in murine male germ cells. Genes Cells 14, 1155-65 (2009)(PMID:19735482)
  5. Federici C et al. Pleiotropic function of ezrin in human metastatic melanomas. Int J Cancer. 124, 2804-12 (2009)(PMID:19235924)
  6. Ozoe F et al. The 14-3-3 proteins Rad24 and Rad25 negatively regulate Byr2 by affecting its localization in Schizosaccharomyces pombe. Mol Cell Biol. 22, 7105-19 (2002)(PMID:12242289)
  7. de Graaf K et al. Characterization of cyclin L2, a novel cyclin with an arginine/serine-rich domain: phosphorylation by DYRK1A and colocalization with splicing factors. J Biol Chem. 279, 4612-24 (2004)(PMID:14623875)
  8. Lozupone F et al. Identification and relevance of the CD95-binding domain in the N-terminal region of ezrin. J Biol Chem. 279, 9199-207 (2004)(PMID:14676203)
  9. Lebel-Haziv Y et al. Breast cancer: coordinated regulation of CCL2 secretion by intracellular glycosaminoglycans and chemokine motifs. Neoplasia 16, 723-40 (2014)(PMID:25246273)
  10. Zhang B et al. GSK3β-Dzip1-Rab8 cascade regulates ciliogenesis after mitosis. PLoS Biol. 13, e1002129 (2015)(PMID:25860027)
  11. Watanabe N et al. Dystonia-4 (DYT4)-associated TUBB4A mutants exhibit disorganized microtubule networks and inhibit neuronal process growth. Biochem Biophys Res Commun. 495, 346-352 (2018)(PMID:29127012)
  12. Li Z et al. Destabilization of linker histone H1.2 is essential for ATM activation and DNA damage repair. Cell Res. 28, 756-770 (2018)(PMID:29844578)

Immunocytochemistry

  1. Banerjee S et al. A coordinated local translational control point at the synapse involving relief from silencing and MOV10 degradation. Neuron. 64, 871-84 (2009)(PMID:20064393)
  2. Asano Y et al. DRR1 is expressed in the developing nervous system and downregulated during neuroblastoma carcinogenesis. Biochem Biophys Res Commun. 394, 829-35 (2010)(PMID:20298674)
  3. Ko HS et al. Role of ubiquilin associated with protein-disulfide isomerase in the endoplasmic reticulum in stress-induced apoptotic cell death. J Biol Chem. 277, 35386-92 (2002)(PMID:12095988)

Immunohistochemistry

  1. Broday L et al. The LIM domain protein UNC-95 is required for the assembly of muscle attachment structures and is regulated by the RING finger protein RNF-5 in C. elegans. J Cell Biol. 165, 857-67 (2004)(PMID:15210732)
  2. Sugawara T et al. Type 1 inositol trisphosphate receptor regulates cerebellar circuits by maintaining the spine morphology of purkinje cells in adult mice. J Neurosci. 33, 12186-96 (2013)(PMID:23884927)
  3. Katsushima K et al. Targeting the Notch-regulated non-coding RNA TUG1 for glioma treatment. Nat Commun. 7, 13616 (2016)(PMID:27922002)
  4. Shainer I et al. Novel hypophysiotropic AgRP2 neurons and pineal cells revealed by BAC transgenesis in zebrafish. Sci Rep. 7, 44777 (2017)(PMID:28317906)

Immunofluorescence

  1. Sun J et al. Histone H1-mediated epigenetic regulation controls germline stem cell self-renewal by modulating H4K16 acetylation. Nat Commun. 6, 8856 (2015)(PMID:26581759)
  2. Shimizu T et al. The C. elegans BRCA2-ALP/Enigma Complex Regulates Axon Regeneration via a Rho GTPase-ROCK-MLC Phosphorylation Pathway. Cell Rep. 24, 1880-1889 (2018)(PMID:30110643)
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※在庫につきましては2019年12月08日 17時00分時点における在庫数を表示してあります。
※価格の赤色表示につきましては、キャンペーン価格の表示となっております。
※このサイトからは直接注文はできません。ご注意ください。
※使用法の表記について:
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation,
FCM: Flow Cytometry NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation,
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
※使用法・交差性の表記について:
*: 論文で報告されております(MBLでは未確認)。詳しくはデータシートをご覧ください。
**: 導入元からの情報です(MBLでは評価中もしくは未確認)。
※保存温度の表記について: RT: 室温
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