トップ > 製品検索結果一覧 > Code No. D345-3 Anti-1-methyladenosine (m1A) mAb

Code No.　D345-3

Anti-1-methyladenosine (m1A) mAb

価格（税別）

¥66,000

在庫

10以上

（2024/04/25 00:05時点）

包装

100 µL (1 mg/mL)

Anti-1-methyladenosine (m1A) mAb (Clone: AMA-2) reacts with 1-methyladenosine (m1A or m1Ado), 1-methyladenine (1-mA) and m1A containing RNA such as tRNA.
データ

  • Immunoprecipitation

  • Immunocytochemistry

  • Immunohistochemistry
クローナリティー Monoclonal クローン AMA-2
アイソタイプ
（免疫動物） 		Mouse IgG2bκ
使用法
IP
15 µg/sample  
IC
1 µg/mL  
IH
0.1 µg/mL  
ELISA**
Inhibition ELISA (Info. from the licenser. Not tested by MBL.)  
DB*
reported.  （PMID: 26863196 / 26863410）
Other(Immuno-Nothern blotting)
  （PMID: 26606401）
免疫原(抗原) KLH-conjugated 1-methyladenosine (KLH-m1A)
性状 1 mg/mL in PBS/50% glycerol, pH 7.2
保存温度 -20°C 標識 Unlabeled メーカー MBL
別称 m1Ado
背景 In eukaryote, newly synthesized RNA is chemically modified at specific nucleotide position after transcription. N1-methyladenosine (m1A) is one of major modified nucleosides present in most eukaryotic tRNAs and also found in yeast rRNAs. m1A affects structural stability of tRNA and its binding to polysome in translation. Urinary excretion of modified nucleosides including m1A is known to be increased in the patients with various cancers and AIDS. It is reported that the amount of urinary m1A correlates with m1A level in tissue from same patients with esophageal carcinoma. Moreover, elevated serum levels of m1A are also detected under stress conditions.
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RN016M Anti-7-methylguanosine (m7G)-Cap mAb
RN017M Anti-7-methylguanosine (m7G) mAb
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使用文献
 使用文献募集中！本製品を使って論文を発表されましたら是非お知らせください。

Dot Blotting

  1. Dominissini D et al. The dynamic N(1)-methyladenosine methylome in eukaryotic messenger RNA. Nature 530, 441-6 (2016)(PMID:26863196)
  2. Li X et al. Transcriptome-wide mapping reveals reversible and dynamic N(1)-methyladenosine methylome. Nat Chem Biol. 12, 311-6 (2016)(PMID:26863410)
  3. Liu F et al. ALKBH1-Mediated tRNA Demethylation Regulates Translation. Cell 167, 816-828.e16. (2016)(PMID:27745969)
  4. Ueda Y et al. AlkB homolog 3-mediated tRNA demethylation promotes protein synthesis in cancer cells. Sci Rep. 7, 42271 (2017)(PMID:28205560)

Northern Blotting

  1. Elkordy A et al. tiRNAs as a novel biomarker for cell damage assessment in in vitro ischemia-reperfusion model in rat neuronal PC12 cells. Brain Res. 1714, 8-17 (2019)(PMID:30779912)

Immunoprecipitation

  1. Dominissini D et al. The dynamic N(1)-methyladenosine methylome in eukaryotic messenger RNA. Nature 530, 441-6 (2016)(PMID:26863196)
  2. Li X et al. Transcriptome-wide mapping reveals reversible and dynamic N(1)-methyladenosine methylome. Nat Chem Biol. 12, 311-6 (2016)(PMID:26863410)
  3. Waku et al. NML-mediated rRNA base methylation links ribosomal subunit formation to cell proliferation in a p53-dependent manner. J. Cell Sci. 129, 2382-93 (2016)(PMID:27149924)
  4. Safra M et al. The m1A landscape on cytosolic and mitochondrial mRNA at single-base resolution. Nature 551, 251-255 (2017)(PMID:29072297)
  5. Li X et al. Base-Resolution Mapping Reveals Distinct m1A Methylome in Nuclear- and Mitochondrial-Encoded Transcripts. Mol Cell  68, 993-1005 (2017)(PMID:29107537)

Immunocytochemistry

  1. Ueda Y et al. AlkB homolog 3-mediated tRNA demethylation promotes protein synthesis in cancer cells. Sci Rep. 7, 42271 (2017)(PMID:28205560)

Other(Immuno-Nothern blotting)

  1. Mishima E et al. Immuno-Northern Blotting: Detection of RNA Modifications by Using Antibodies against Modified Nucleosides. PLoS One 10, e0143756 (2015)(PMID:26606401)
  2. Alaa E et al. Stress-induced tRNA cleavage and tiRNA generation in rat neuronal PC12 cells. J Neurochem. (2018) In press.(PMID:29431851)
  3. Mishima E and Abe T. Immuno-Northern Blotting: Detection of Modified RNA Using Gel Separation and Antibodies to Modified Nucleosides. Methods Mol Biol. 1870, 179-187 (2019) (PMID:30539555)
  4. Sato K et al. Stress Induced tRNA Halves (tiRNAs) as Biomarkers for Stroke and Stroke Therapy; Pre-clinical Study. Neuroscience. 434, 44-54 (2020)(PMID:32200075)
参考文献
  1. Itoh K et al. Preparation of a monoclonal antibody specific for 1-methyladenosine and its application for the detection of elevated levels of 1-methyladenosine in urines from cancer patients. Jpn J Cancer Res. 79, 1130-8 (1988)（PMID: 3143701）
  2. Itoh K et al. Diagnostic use of anti-modified nucleoside monoclonal antibody. Tohoku J Exp Med. 168, 329-31 (1992)1306318（PMID: 1306318）
  3. Masuda M et al. An immunohistochemical analysis for cancer of the esophagus using monoclonal antibodies specific for modified nucleosides.Cancer 72, 3571-8 (1993)（PMID: 8252470）
  4. Mishima E et al. Conformational Change in Transfer RNA Is an Early Indicator of Acute Cellular Damage. J Am Soc Nephrol. 25, 2316-26 (2014)（PMID: 24833129）
  5. Chi KR. The RNA code comes into focus. Nature 542, 503-506 (2017)（PMID: 28230128）
製品
カテゴリー
分野
RNA-RNPネットワーク
エピジェネティクス
がん
細胞ストレス
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※在庫につきましては2024年04月25日 00時05分時点における在庫数を表示してあります。
※価格の赤色表示につきましては、キャンペーン価格の表示となっております。
※このサイトからは直接注文はできません。ご注意ください。
※使用法の表記について：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation,
FCM: Flow Cytometry NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation,
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
※使用法・交差性の表記について：
*: 論文で報告されております（MBLでは未確認）。詳しくはデータシートをご覧ください。
**: 導入元からの情報です（MBLでは評価中もしくは未確認）。
※保存温度の表記について： RT: 室温
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