トップ > 製品検索結果一覧 > Code No. D048-3M2 Anti-IL-18 (Mouse) mAb (Functional Grade)

Code No. D048-3M2

Anti-IL-18 (Mouse) mAb (Functional Grade)

価格(税別)

¥72,000

在庫

10以上

(2019/12/07 00:05時点)

包装

100 µg/100 µL

データ
  • Immunoprecipitation

  • Neutralization

    Induction of IFN-γ by mouse IL-18 receptor transfected KG-1 cell (KG-1 cell: Human myelomonocyte: ATCC CCL246) in response to the 30 ng/mL recombinant mouse IL-18 was neutralized by this antibody. This table shows neutralization activity of lot 001.

クローナリティー Monoclonal クローン 93-10C
アイソタイプ
(免疫動物)
Rat IgG1
使用法
IP
2 μg/0.2 μg of recombinant mouse IL-18  
NT
0.5-5 µg/mL  
免疫原(抗原) Mouse IL-18 (recombinant)
交差性
[Gene ID]

Mouse[16173]

性状 1 mg/mL in PBS. Azide free, 0.22 μm sterile-filtered
保存温度 -20°C 標識 Unlabeled メーカー MBL
別称 interleukin 18 (interferon-gamma-inducing factor), IGIF, IL1F4, IL-1g, IL-18
背景 Interleukin 18 (IL-18) is a 18 kDa cytokine which identified as a costimulatory factor for production of interferon-γ (IFN-γ) in response to toxic shock and shares functional similarities with IL-12. IL-18 is synthesized as a precursor 24 kDa molecule without a signal peptide and must be cleaved to produce an active molecule. IL-1 converting enzyme (ICE, Caspase-1) cleaves pro-IL-18 at aspartic acid in the P1 position, producing the mature, bioactive peptide that is readily released from the cells. It is reported that IL-18 is produced from Kupffer cells, activated macrophages, keratinocytes, intestinal epithelial cells, osteoblasts, adrenal cortex cells and murine diencephalon. IFN-γ is produced by activated T or NK cells and plays critical roles in the defense against microbiral pathogens. IFN-γ activates macrophages, enhances NK activity and B cell maturation, proliferation and Ig secretion, induces MHC class I and II antigens, and inhibits osteoclast activation. IL-18 acts on T helper type-1 (Th1) T cells and in combination with IL-12 strongly induces them to produce IFN-γ. Pleiotropic effects of IL-18 has also been reported, such as, enhancement production of IFN-γ and GM-CSF in peripheral blood mononuclear cells, production of Th1 cytokines, IL-2, GM-CSF and IFN-γ in T cells, enhancement of Fas ligand expression by Th1 cells.
関連製品 D048-3 Anti-IL-18 (Mouse) mAb
D048-6 Anti-IL-18 (Mouse) mAb-Biotin
D046-3 Anti-IL-18 (Mouse) mAb
D047-3 Anti-IL-18 (Mouse) mAb
7625 Mouse IL-18 ELISA Kit
B002-5 Recombinant Mouse IL-18
B004-5 Recombinant Mouse IL-18 (without BSA)
M080-3M2 Rat IgG1 (isotype control) (Functional Grade)
D045-3 Anti-IL-18 (Human) mAb
参考文献
  1. Okamura H et al. Cloning of a new cytokine that induces IFN-gamma production by T cells. Nature 378, 88-91 (1995)(PMID: 7477296
  2. Ushio S et al. Cloning of the cDNA for human IFN-gamma-inducing factor, expression in Escherichia coli, and studies on the biologic activities of the protein. J Immunol. 156, 4274-9 (1996)(PMID: 8666798
  3. Micallef M et al. Interferon-gamma-inducing factor enhances T helper 1 cytokine production by stimulated human T cells: synergism with interleukin-12 for interferon-gamma production. Eur J Immunol. 26, 1647-51 (1996)(PMID: 8766574
  4. Dao T et al. Interferon-gamma-inducing factor, a novel cytokine, enhances Fas ligand-mediated cytotoxicity of murine T helper 1 cells. Cell Immunol. 173, 230-5 (1996)(PMID: 8912881
  5. Rogers TB et al. Mesenchymal stem cells stimulate protective genetic reprogramming of injured cardiac ventricular myocytes. J Mol Cell Cardiol. 50, 346-56 (2011)(PMID: 20837021
  6. Zanoni I et al. IL-15 cis presentation is required for optimal NK cell activation in lipopolysaccharide-mediated inflammatory conditions. Cell Rep. 26, 1235-49 (2013)(PMID: 24055061
  7. Hirano Y et al. IL-18 is not therapeutic for neovascular age-related macular degeneration. Nat Med. 20, 1372-5 (2014)(PMID: 25473914
  8. Abdel Fattah E et al. Critical role for IL-18 in spontaneous lung inflammation caused by autophagy deficiency. J Immunol. 194, 5407-16 (2015)(PMID: 25888640
製品
カテゴリー
分野
免疫
サイトカイン・増殖因子
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※在庫につきましては2019年12月07日 00時05分時点における在庫数を表示してあります。
※価格の赤色表示につきましては、キャンペーン価格の表示となっております。
※このサイトからは直接注文はできません。ご注意ください。
※使用法の表記について:
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation,
FCM: Flow Cytometry NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation,
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
※使用法・交差性の表記について:
*: 論文で報告されております(MBLでは未確認)。詳しくはデータシートをご覧ください。
**: 導入元からの情報です(MBLでは評価中もしくは未確認)。
※保存温度の表記について: RT: 室温
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