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  1. How do you check the BrU incorporation rate in the cells ?
    The BrU incorporation rate can be confirmed by the following methods:
    ・Flow cytometry using BrU-labeled cells
    ・RNA-IP, Dot blot, or ELISA using total RNA derived from BrU-labeled cells
    Please select the method best suited for your purpose.
  2. BrU is not efficiently incorporated into the cells.
    Please optimize the BrU concentraiton, pulse-time, or chase-time. BrU concentrations of more than 500 μM may affect cell growth and/or other biological processes.
  3. BrU-labeled RNA cannot be corrected.
    Please optimize the amount of input, antibody, or beads.