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Protein purification kit application note Vol.1




- Pull-down assay -

 


Introduction of Pull-down assay experiments using Tagged protein purification kits

 

Proteins function by interacting with various other proteins. To reveal the function of a protein, it is important to Identifiy other proteins which interact to the protein.

Protocol

Experiment conditions

Exampe 1: Pull-down assay of an endogenous protein

 

p62/SQSTM1 directly interacts with LC3, which is localized on autophagosome membrane, and is degradated by autophagic-lysosome pathway. Interactions of p62/SQSTM1 with LC3 was confirmed by pull-down assay using the cell lysate of the HA-LC3 transfectant.

HA tagged Protein PURIFICATION KIT (MBL code no.3320)

Example 2: Pull-down assay of forcibly expressed proteins

 

Beclin 1, which is essential for early autophagosome formation, is reported to make complex with various proteins such as UBRAG and Vps34 and regulate the processes of autophagosome/lysosome fusion (Autophagosome maturation) and endocytosis. Using HA, c-Myc, and His-tagged protein purification kits, pull-down assay of Beclin1 and UVRAG is performed (Samples: HA tagged Beclin1/Myc-His tagged UVRAG transfectant).

HA tagged Protein PURIFICATION KIT (MBL code no.3320)

c-Myc tagged Protein MILD PURIFICATION KIT Ver.2 (MBL code no.3305)

His tagged Protein PURIFICATION KIT (MBL code no.3310)



Products

 
Code No. Product Quantity
3305 c-Myc-tagged Protein Mild Purification Kit Ver.2 20 tests
3305A c-Myc-tagged Protein Mild Purification Kit Ver.2
(Trial Kit)
2 tests
3310 His-tagged Protein Purification Kit 20 tests
3310A His-tagged Protein Purification Kit Trial Kit 2 tests
3315 V5-tagged Protein Purification Kit 20 tests
3315A V5-tagged Protein Purification Kit Trial Kit 2 tests
3320 HA-tagged Protein Purification KIT 20 tests
3320A HA-tagged Protein Purification KIT Trial Kit 2 tests
3325 DDDDK-tagged Protein Purification Kit 20 tests
3325A DDDDK-tagged Protein Purification Kit Trial Kit 2 tests



■Protein purification kit application note Vol.1
PDF ver.: 292KB (in Japanese)




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