Organelle labeling tools

• Organelle Specific Antibodies
• CoralHue™ Organelle Targeting Vectors

Protein function is dependent on intracellular localization. Therefore, it is useful to identify the location of the targeted proteins in cells in order to understand the biological phenomenon. The organelle-specific antibodies developed by MBL can be used as markers to study the intracellular localization of the target protein. These antibodies can be used for localization analysis of the target proteins on multicolor staining of the cell or with western blotting of cell fractions.

Early endosome

M176-3 anti-EEA1	PM062 anti-EEA1

Endoplasmic reticulum (ER)

M181-3 anti-KDEL

Golgi body

M179-3 anti-GM130	PM061 anti-GM130

Mitochondria

PM063 anti-COX4

Nuclear membrane

PM064 anti-Lamin B1

Centromere

D115-3 anti-CENP-A

Autophagosome

PM036 anti-LC3	M152-3 anti-LC3

α-Tubulin

M175-3 anti-α-Tubulin	PM054 anti-α-Tubulin

- Available for fluorescent labeling of living cells -

CoralHue™ Organelle Targeting Vector is a plasmid to express fluorescent protein labeling organelles specifically. It is designed to express fluorescent proteins in the targeted organelle by using the localized signal sequence of each organelle. It is possible to label each organelle individually as required. The vector also contains the gene for neomycin-resistance, enabling homeostatis labeling of each organelle by drug selection.

※CoralHue™ fluorescent proteins were co-developed by Amalggam and the Laboratory for Cell Function and Dynamics, Advanced Technology Development Center, Brain Science Institute, and Institute of Physical and Chemical Research RIKEN (lab head, Dr. Atsushi Miyawaki). The use of CoralHue™ fluorescent proteins requires a license agreement from Amalggam.