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HOME > Product search results > Code No. MEX-USB ExoCap™ Ultracentrifugation/Storage Booster

Code No. MEX-USB

ExoCap™ Ultracentrifugation/Storage Booster

Availability (in Japan)

10 or more

(In Japan at 01:30,
Oct 18, 2017 in JST)

Size

50 mL

  • Can Improve the recovery rate of exosomes from cell culture supernatant by ultracentrifugation
  • Can Improve the storage stability of the purified exosomes under low temperature conditions
Data
  • Evaluation of the ExoCap™ USB effect for ultracentrifugation by Western blotting (Non-reduced)

    Evaluation of the ExoCap™ USB effect for ultracentrifugation by Western blotting (Non-reduced)
    HT29 cells were cultured up to 60-80%. Subsequently, wash the cells and change the new medium without FBS. 3 days later, recover the supernatant. The cell density was reached from 90% to confluent. After the pre-clearance by centrifugation at 300-3,000 × g, this supernatant was filtrated by 0.22 µm filter and then used for the experiment.  Exosome was isolated from the supernatant by ultracentrifugation with/without ExoCap™ USB. The intensity of the bands of exosome markers with the ExoCap™ USB was denser than those of without ExoCap™ USB. Exosome recovery was boosted by using the ExoCap™ USB.

  • Evaluation of the ExoCap™ USB effect for ultracentrifugation by CLEIA

    Evaluation of the ExoCap™ USB effect for ultracentrifugation by CLEIA
    Exosome recovery rate was improved as a result of the ExoCap USB addition. For beads CLEIA (Chemiluminescent Enzyme Immuno Assay) ExoCap™ Streptavidin Kits, biotinylated CD81 antibody and alkaline phosphatase (ALP) labeled CD9 antibody were used.

Components 50 mL of soluble additive
Intended use This reagent can be available for efficient exosome purification by ultracentrifugation. It can be used as the additive in order to improve the recovery rate of exosome in cultured supernatant by ultracentrifugation. Furthermore, it can also improve the storage stability of purified exosome under low temperature conditions.
Storage temp. 4°C Manufacturer MBL
Background ExoCap™ Ultracentrifugation/Storage Booster (ExoCap™ USB) is an optimized additive and available for two applications. It can be used for efficient exosome purification by ultracentrifugation from cultured supernatant and improve the storage stability of purified exosome under low temperature conditions.   This reagent does not contain any protein components.
Related products EX-C9-SP ExoCap™ CD9 Kit for Serum Plasma
EX-C63-SP ExoCap™ CD63 Kit for Serum Plasma
EX-C81-SP ExoCap™ CD81 Kit for Serum Plasma
EX-COM-SP ExoCap™ Composite Kit for Serum Plasma
EX-EPC-SP ExoCap™ EpCAM Kit for Serum Plasma
MEX001-3 Anti-CD9 mAb
MEX002-3 Anti-CD63 (LAMP-3) mAb
MEX003-3 Anti-CD81 (TAPA1) mAb
MEX-E ExoCap™ Nucleic Acid Elution Buffer
MEX001-6 Anti-CD9 mAb-Biotin
MEX002-6 Anti-CD63 (LAMP-3) mAb-Biotin
MEX003-6 Anti-CD81 (TAPA1) mAb-Biotin
MEX004-6 Anti-CD326 (EpCAM) mAb-Biotin
MEX-SA ExoCap™ Streptavidin Kit
MEX001-12 Anti-CD9 mAb-ALP
MEX002-12 Anti-CD63 (LAMP-3) mAb-ALP
MEX003-12 Anti-CD81 (TAPA1) mAb-ALP
MEX1001 ExoDiluent for Immunoassay
Notes If precipitation occurs during storage, equilibrate the reagent to room temperature and mix well to re-dissolve the precipitation.
Product category
Research area
RNA-RNP Network
Cancer
Exosomes
Brands
ExoCap™
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  • The availability is based on the information in Japan at 01:30, Oct 18, 2017 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.