Evaluation of the ExoCap™ USB effect for ultracentrifugation by Western blotting (Non-reduced)
HT29 cells were cultured up to 60-80%. Subsequently, wash the cells and change the new medium without FBS. 3 days later, recover the supernatant. The cell density was reached from 90% to confluent. After the pre-clearance by centrifugation at 300-3,000 × g, this supernatant was filtrated by 0.22 µm filter and then used for the experiment. Exosome was isolated from the supernatant by ultracentrifugation with/without ExoCap™ USB. The intensity of the bands of exosome markers with the ExoCap™ USB was denser than those of without ExoCap™ USB. Exosome recovery was boosted by using the ExoCap™ USB.
Evaluation of the ExoCap™ USB effect for ultracentrifugation by CLEIA
Exosome recovery rate was improved as a result of the ExoCap USB addition. For beads CLEIA (Chemiluminescent Enzyme Immuno Assay) ExoCap™ Streptavidin Kits, biotinylated CD81 antibody and alkaline phosphatase (ALP) labeled CD9 antibody were used.
||50 mL of soluble additive
||This reagent can be available for efficient exosome purification by ultracentrifugation. It can be used as the additive in order to improve the recovery rate of exosome in cultured supernatant by ultracentrifugation. Furthermore, it can also improve the storage stability of purified exosome under low temperature conditions.
||ExoCap™ Ultracentrifugation/Storage Booster (ExoCap™ USB) is an optimized additive and available for two applications. It can be used for efficient exosome purification by ultracentrifugation from cultured supernatant and improve the storage stability of purified exosome under low temperature conditions. This reagent does not contain any protein components.
||MEX001-3 Anti-CD9 mAb
MEX002-3 Anti-CD63 (LAMP-3) mAb
MEX003-3 Anti-CD81 (TAPA1) mAb
MEX-E ExoCap™ Nucleic Acid Elution Buffer
MEX001-6 Anti-CD9 mAb-Biotin
MEX002-6 Anti-CD63 (LAMP-3) mAb-Biotin
MEX003-6 Anti-CD81 (TAPA1) mAb-Biotin
MEX004-6 Anti-CD326 (EpCAM) mAb-Biotin
MEX-SA ExoCap™ Streptavidin Kit
MEX001-12 Anti-CD9 mAb-ALP
MEX002-12 Anti-CD63 (LAMP-3) mAb-ALP
MEX003-12 Anti-CD81 (TAPA1) mAb-ALP
MEX1001 ExoDiluent for Immunoassay
||If precipitation occurs during storage, equilibrate the reagent to room temperature and mix well to re-dissolve the precipitation.
- Research area
- RNA-RNP Network