Exosome isolation using biotin-labeled antibodies
Isotype controls (IgG1, IgG2a, IgG2b and IgG3) have no band. ExoCap™ Streptavidin Kit and specific antibodies (CD9 and CD63) can capture specific targets.
Detection of HT29-derived exosomes captured by ExoCap™ Streptavidin Kit
Exosomes isolated by ExoCap™ Streptavidin Kit from 0.025 µL cell culture supernatant can be detected by swCLEIA.
Detection of miR-21 by using qRT-PCR protocol for ExoCap™ Streptavidin Kit
||Streptavidin Magnetic Beads (2 mL), Treatment Buffer (30 mL), Washing/Dilution Buffer (60 mL)
||ExoCap™ Streptavidin Kit allows for exosome isolation from serum, plasma and cell culture supernatant. Isolated exosomes can be used for western blotting, beads CLEIA, flow cytometry and qRT-PCR.
||Serum, plasma and cell-cultured supernatant
||ExoCap™ Streptavidin Kit is designed for the customized isolation and analysis of exosomes or microvesicles, called “Extracellular Vesicles (EVs)”, using the researcher’s biotinylated molecules such as antibodies against exosome surface marker proteins, of which representatives are tetraspanins such as CD9 and CD63. Exosomes are extracellular vesicles secreted by most cell types and contain various marker proteins and RNAs, such as microRNA and fragmented mRNA. ExoCap™ Streptavidin Kit uses functionalized Magnosphere™ magnetic microparticles for exosome isolation. These beads are coated with a JSR Life Sciences proprietary hydrophilic polymer to decrease non-specific binding.
||MEX001-6 Anti-CD9 mAb-Biotin
MEX002-6 Anti-CD63 (LAMP-3) mAb-Biotin
MEX003-6 Anti-CD81 (TAPA1) mAb-Biotin
MEX004-6 Anti-CD326 (EpCAM) mAb-Biotin
M075-6 Mouse IgG1 (isotype control)-Biotin
M076-6 Mouse IgG2a (isotype control)-Biotin
M077-6 Mouse IgG2b (isotype control)-Biotin
M078-6 Mouse IgG3 (isotype control)-Biotin
MEX-E ExoCap™ Nucleic Acid Elution Buffer
MEX1001 ExoDiluent for Immunoassay
MEX-USB ExoCapTM Ultracentrifugation/Storage Booster
MEX001-3 Anti-CD9 mAb
MEX002-3 Anti-CD63 (LAMP-3) mAb
MEX003-3 Anti-CD81 (TAPA1) mAb
- Research area