Antibody detailed product information

Code No.	RN024P
Anti-PCBP1 pAb
Size	200 µL (1 mg/mL)
Availability (in Japan)	0 (Contact us) （In Japan at 00:05, Apr 17, 2024 in JST）
Clonality	Polyclonal
Clone	Polyclonal
Isotype (Immunized Animal)	Rabbit Ig (aff.)
Applications	WB　1 µg/mL IP　5 µg/500 µL of cell extract from 5 x 10⁶ cells IC*　reported. （PMID: 26990993） RIP　15 µg/500 µL of cell extract from 1.5 x 10⁷ cells Other(EMSA)　reported. （PMID: 27220277）
Immunogen (Antigen)	KLH-conjugated synthetic peptide, corresponding to internal region of human PCBP1
Reactivity [Gene ID]	Human[5093], Mouse[23983], Rat[500242]
Storage buffer	1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp.	-20°C
Conjugate	Unlabeled
Manufacturer	MBL
Alternative names	poly(rC) binding protein 1, HNRPE1, HNRPX, hnRNP-E1, hnRNP-X
Background	Poly(rC)-binding proteins (PCBPs) are cellular RNA-binding proteins; these proteins are defined by their triple KH structure and poly(C)-binding specificity. The 3 KH domains are not only involved in nucleic acid binding but also in mediating protein-protein interactions within the RNP complexes. PCBPs bind specifically to polypyrimidine tracts within the 3′ UTR of target mRNAs through the KH domains. PCBPs are expressed as 4 isoforms in cells. PCBP1 and PCBP2 are widely expressed in both the nucleus and the cytoplasm and can shuttle from the nucleus to the cytoplasm, while PCBP3 and PCBP4 are expressed to a less extent. PCBP1 and PCBP2 have been reported to stabilize the target mRNAs such as those of α-globin, α1(I) collagen, and tyrosine hydroxylase by binding to C-rich 3′ UTR motifs. The 15-lipoxygenase mRNA is translationally silent until reticulocytes in the peripheral blood undergo the final steps of maturation. This translational silencing has been shown to be mediated by the binding of PCBP1 and hnRNPK to the 3′ UTR of 15-lipoxygenase mRNA.
Related products	RN1001 RIP-Assay Kit RN1005 RIP-Assay Kit for microRNA RN052PW Anti-HNRNPC pAb RN060PW Anti-HNRNPD (AUF1) pAb RN019P Anti-HNRNPK pAb RN025P Anti-PCBP2 pAb RN011P Anti-PTBP1 (Human) pAb RN002P Anti-eIF4G1 (Human) pAb RN114PW Anti-HNRNPA1 pAb
Product category	Research area:RNA-RNP Network Cancer Brands:RiboCluster Profiler™
Data
Citations	Western Blotting Espinoza-Lewis RA et al. Poly(C)-binding protein 1 (Pcbp1) regulates skeletal muscle differentiation by modulating microRNA processing in myoblasts. J Biol Chem. 292, 9540-9550 (2017)(PMID:28381556) Immunocytochemistry Sundararaman B et al. Resources for the Comprehensive Discovery of Functional RNA Elements. Mol Cell. 61, 903-13 (2016)(PMID:26990993) RNP Immunoprecipitation Yabe-Wada T et al. TLR signals posttranscriptionally regulate the cytokine trafficking mediator sortilin. Sci. Rep. 6, 26566 (2016)(PMID:27220277) Other(EMSA) Yabe-Wada T et al. TLR signals posttranscriptionally regulate the cytokine trafficking mediator sortilin. Sci. Rep. 6, 26566 (2016)(PMID:27220277)

The availability is based on the information in Japan at 00:05, Apr 17, 2024 in JST.
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Abbreviations for applications：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
For applications and reactivity:
*: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
**: The use is reported from the licenser (Under evaluation or not tested by MBL).
For storage temparature: RT: room temparature
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