Antibody detailed product information

Code No.	RN014P
Anti-TIA1 pAb
Size	200 µL (1 mg/mL)
Availability (in Japan)	10 or more （In Japan at 00:05, Apr 19, 2024 in JST）
Clonality	Polyclonal
Clone	Polyclonal
Isotype (Immunized Animal)	Rabbit Ig (aff.)
Applications	WB　1:1,000 IP　5 µg/250 µL of cell extract from 2.5 x 10⁶ cells IC　 RIP　15 µg/500 µL of cell extract from 6 x 10⁶ cells Other(eCLIP)　reported. （PMID: 27018577）
Immunogen (Antigen)	KLH-conjugated synthetic peptide NGSMLPNQPSGYRVAGYETQ （367-386 aa)
Reactivity [Gene ID]	Human[7072], Mouse[21841], Rat[312510], Hamster
Storage buffer	1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp.	-20°C
Conjugate	Unlabeled
Manufacturer	MBL
Alternative names	TIA1 cytotoxic granule-associated RNA binding protein, WDM, TIA-1
Background	T-cell intracellular antigen-1 (TIA1), an apoptosis promoting factor, is a RNA binding protein which associates with subsets of mRNAs bearing U-rich sequences in their 3’-UTRs and shuttles between nucleus and cytoplasm. TIA1 possesses three RNA recognition motifs (RRMs). RRM2 and the first half of the auxiliary region are necessary for nuclear accumulation whereas RRM3 mediates nuclear export. TIA1 regulates splicing of alternatively spliced pre-mRNAs including Fas, msl-2, FGFR-2, calsitonin/CGRP in nucleus. In cytoplasm, TIA1 regulates translation of various mRNAs, include TNF-α, Cox-2, HMMP-13 and β2-adrenergic receptor, by binding to AU-rich elements (AREs) located in these mRNA 3’ untranslated regions (3’UTRs). In addition to the translational silencing, TIA1 plays a role in the cellular response to environmental stress by promoting the assembly of a non-canonical 48S preinitiation complex that is the core component of cytoplasmic stress granules (SG). Like other mRNA turnover and translation regulators such as AUF-1, HuR, KSRP and NF90, TIA1 also associates its cognate mRNA and transcripts of other RBPs.
Related products	RN1001 RIP-Assay Kit RN1005 RIP-Assay Kit for microRNA RN059PW Anti-TIAL1 pAb
Product category	Research area:RNA-RNP Network Brands:RiboCluster Profiler™
Data
Citations	Western Blotting Liu W et al. Hepatitis B virus core protein inhibits Fas-mediated apoptosis of hepatoma cells via regulation of mFas/FasL and sFas expression. FASEB J. 29, 1113-23 (2015)(PMID:25466893) Arimoto-Matsuzaki K et al. TIA1 oxidation inhibits stress granule assembly and sensitizes cells to stress-induced apoptosis. Nat Commun. 7, 10252 (2016) (PMID:26738979 ) RNP Immunoprecipitation Arimoto-Matsuzaki K et al. TIA1 oxidation inhibits stress granule assembly and sensitizes cells to stress-induced apoptosis. Nat Commun. 7, 10252 (2016) (PMID:26738979) Other(eCLIP) Van Nostrand EL et al. Robust transcriptome-wide discovery of RNA-binding protein binding sites with enhanced CLIP (eCLIP). Nat Methods. 13, 508-14 (2016)(PMID:27018577)

The availability is based on the information in Japan at 00:05, Apr 19, 2024 in JST.
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Abbreviations for applications：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
For applications and reactivity:
*: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
**: The use is reported from the licenser (Under evaluation or not tested by MBL).
For storage temparature: RT: room temparature
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