Antibody detailed product information

Code No. RN007P
Anti-IGF2BP1 (IMP1) pAb
Size 200 µL (1 mg/mL)
Availability (in Japan) 10 or more
（In Japan at 17:33, Apr 26, 2024 in JST）
Clonality Polyclonal
Clone Polyclonal
Isotype
(Immunized Animal) 		Rabbit Ig (aff.)
Applications WB　1:1,000  
IP　5 µg/250 µL of cell extract from 2.5 x 106 cells  
IC　Can be used.  
RIP　15 µg/500 µL of cell extract from 1.2 x 107 cells  
Other(eCLIP)　reported.  （PMID: 27018577 / 27068461）
Other(iCLIP)　reported.  （PMID: 29245011）
Immunogen
(Antigen)		 KLH-conjugated synthetic peptide KQQHQKGQSNQAQARRK（561-577 aa)
Reactivity [Gene ID]

Human[10642], Mouse[140486](PMID: 22215810), Rat(-), Hamster(-)
Storage buffer 1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp. -20°C
Conjugate Unlabeled
Manufacturer MBL
Alternative names insulin-like growth factor 2 mRNA binding protein 1, ZBP1, CRDBP, IMP-1, CRD-BP, VICKZ1/ Zbp1, Crdbp
Background The localization of IGF2 mRNA-binding protein 1 (IMP1) is associated with motility and the major functions of IMP1 are carried out by the phylogenetically conserved KH domains. IMP1 can affect stability, localization, and translation of its target RNAs. Binding of IMP1 to the leader 3 mRNA in the 5’-UTR of IGF2 inhibits translation from a leader 3 reporter mRNA. Aberrant IMP1 expression may interfere with c-myc regulation. Compared with copy number gains at 17q21 (IMP1 locus), gains at 8q24 (c-myc locus) were observed more frequently in tumors. Furthermore, IMP1 expression was frequently detected in tumors, implying mechanism of activation other than gene amplification.
Related products RN1001 RIP-Assay Kit
RN1005 RIP-Assay Kit for microRNA
RN001M Anti-IGF2BP1 (IMP1) mAb
RN008P Anti-IGF2BP2 (IMP2) pAb
RN009P Anti-IGF2BP3 (IMP3) pAb
RN048PW Anti-G3BP1 pAb
Product category Research area:RNA-RNP Network Neuroscience
Brands:RiboCluster Profiler™
Data



Citations

Western Blotting

  1. King DT et al. Assessing specific oligonucleotides and small molecule antibiotics for the ability to Inhibit the CRD-BP-CD44 RNA interaction. PLoS One 9, e91585 (2014)(PMID:24622399)
  2. Conway AE et al. Enhanced CLIP uncovers IMP Protein-RNA targets in human pluripotent stem cells Important for cell adhesion and survival. Cell Rep. 15, 666-79 (2016)(PMID:27068461)
  3. Donlin-Asp PG et al. The Survival of Motor Neuron Protein Acts as a Molecular Chaperone for mRNP Assembly. Cell Rep. 18, 1660-1673 (2017)(PMID:28199839)
  4. JnBaptiste CK et al. Dicer loss and recovery induce an oncogenic switch driven by transcriptional activation of the oncofetal Imp1-3 family. Genes Dev. 31, 674-687 (2017)(PMID:28446596)
  5. Hosono Y et al. Oncogenic Role of THOR, a Conserved Cancer/Testis Long Non-coding RNA. Cell 171, 1559-1572 (2017)(PMID:29245011)

Immunoprecipitation

  1. Stoskus M et al. ETV6/RUNX1 transcript is a target of RNA-binding protein IGF2BP1 in t(12;21)(p13;q22)-positive acute lymphoblastic leukemia. Blood Cells Mol Dis. 57, 30-4 (2016)(PMID:26852652)
  2. Conway AE et al. Enhanced CLIP uncovers IMP Protein-RNA targets in human pluripotent stem cells Important for cell adhesion and survival. Cell Rep. 15, 666-79 (2016)(PMID:27068461)
  3. Hosono Y et al. Oncogenic Role of THOR, a Conserved Cancer/Testis Long Non-coding RNA. Cell 171, 1559-1572 (2017)(PMID:29245011)

Immunocytochemistry

  1. Sundararaman B et al. Resources for the Comprehensive Discovery of Functional RNA Elements. Mol Cell. 61, 903-13 (2016)(PMID:26990993)
  2. Donlin-Asp PG et al. The Survival of Motor Neuron Protein Acts as a Molecular Chaperone for mRNP Assembly. Cell Rep. 18, 1660-1673 (2017)(PMID:28199839)
  3. Markmiller S et al. Context-Dependent and Disease-Specific Diversity in Protein Interactions within Stress Granules. Cell 172, 590-604.e13 (2018)(PMID:29373831)

RNP Immunoprecipitation

  1. Patel VL et al. Spatial arrangement of an RNA zipcode identifies mRNAs under post-transcriptional control. Genes Dev. 26, 43-53 (2012)(PMID:22215810)
  2. King DT et al. Assessing specific oligonucleotides and small molecule antibiotics for the ability to Inhibit the CRD-BP-CD44 RNA interaction. PLoS One 9, e91585 (2014)(PMID:24622399)
  3. Conway AE et al. Enhanced CLIP uncovers IMP Protein-RNA targets in human pluripotent stem cells Important for cell adhesion and survival. Cell Rep. 15, 666-79 (2016)(PMID:27068461)
  4. Donlin-Asp PG et al. The Survival of Motor Neuron Protein Acts as a Molecular Chaperone for mRNP Assembly. Cell Rep. 18, 1660-1673 (2017)(PMID:28199839)
  5. de Vasconcellos JF et al. IGF2BP1 overexpression causes fetal-like hemoglobin expression patterns in cultured human adult erythroblasts. PNAS. 114, E5664-E5672 (2017)(PMID:28652347)
  6. Hosono Y et al. Oncogenic Role of THOR, a Conserved Cancer/Testis Long Non-coding RNA. Cell 171, 1559-1572 (2017)(PMID:29245011)

Other(eCLIP)

  1. Conway AE et al. Enhanced CLIP uncovers IMP Protein-RNA targets in human pluripotent stem cells Important for cell adhesion and survival. Cell Rep. 15, 666-79 (2016)(PMID:27068461)
  2. Van Nostrand EL et al. Robust transcriptome-wide discovery of RNA-binding protein binding sites with enhanced CLIP (eCLIP). Nat Methods. 13, 508-14 (2016)(PMID:27018577)

Other(iCLIP)

  1. Hosono Y et al. Oncogenic Role of THOR, a Conserved Cancer/Testis Long Non-coding RNA. Cell 171, 1559-1572 (2017)(PMID:29245011)
  • The availability is based on the information in Japan at 17:33, Apr 26, 2024 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications：
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
    DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.
MBLCopyright (C)2015 Medical ＆ Biological Laboratories Co., Ltd. All Rights Reserve