Antibody detailed product information

Code No. RN003P
Anti-EIF4G2 pAb
Size 200 µL (1 mg/mL)
Availability (in Japan) 10 or more
（In Japan at 00:05, Apr 20, 2024 in JST）
Clonality Polyclonal
Clone Polyclonal
Isotype
(Immunized Animal) 		Rabbit Ig (aff.)
Applications WB　1:1,000  
IP　5 µg/250 µL of cell extract from 2.5 x 106 cells  
IC*　reported.  （PMID: 26990993）
RIP　15 µg/500 µL of cell extract from 4.5 x 106 cells  
Other(eCLIP)　reported.  （PMID: 27018577）
Immunogen
(Antigen)		 KLH-conjugated synthetic peptide EVNPPSDETDSSSAPSKE (783-800 aa)
Reactivity [Gene ID]

Human[1982], Mouse[13690], Rat[361628], Hamster[100758677]
Storage buffer 1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp. -20°C
Conjugate Unlabeled
Manufacturer MBL
Alternative names eukaryotic translation initiation factor 4 gamma 2, OK/SW-cl.75, AAG1, DAP5, NAT1, P97
Background The eukaryote polypeptide chain initiation factor eIF4G forms complex with the mRNA cap-binding protein eIF4E and the ATP dependent RNA helicase eIF4A, constituting the initiation factor eIF4F complex. The eIF4G plays an important role in the mechanism of translation by tethering between other components of the ribosomal initiation factors. Two forms of eIF4G have been reported as two different gene products, eIF4G1 and 2. The eIF4G2 was identified as a functional homologue of eIF4G1. The homology of eIF4G2 to eIF4G1 is particularly high in central and carboxyl portions which corresponds to the eIF4A and eIF3 binding region. The proteolysis of both eIF4G1 and eIF4G2 is associated with a decrease in cell viability and the inhibition of protein synthesis.
Related products RN1001 RIP-Assay Kit
RN1005 RIP-Assay Kit for microRNA
RN001P Anti-EIF4E pAb
RN002P Anti-eIF4G1 (Human) pAb
RN004M Anti-Ribosomal P0/P1/P2 mAb
RN006M Anti-EIF4E mAb
Product category Research area:RNA-RNP Network
Brands:RiboCluster Profiler™
Data



Citations

Immunocytochemistry

  1. Sundararaman B et al. Resources for the Comprehensive Discovery of Functional RNA Elements. Mol Cell. 61, 903-13 (2016)(PMID:26990993)

Other(eCLIP)

  1. Van Nostrand EL et al. Robust transcriptome-wide discovery of RNA-binding protein binding sites with enhanced CLIP (eCLIP). Nat Methods. 13, 508-14 (2016)(PMID:27018577)
  • The availability is based on the information in Japan at 00:05, Apr 20, 2024 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications：
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
    DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.
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