Antibody detailed product information

Code No.	PM075
Anti-GNAT2 (Zebrafish) pAb
Size	100 µL
Availability (in Japan)	1-9 （In Japan at 17:00, Apr 24, 2024 in JST）
Clonality	Polyclonal
Clone	Polyclonal
Isotype (Immunized Animal)	Rabbit Ig (aff.)
Applications	WB　1:1,000-1:2,000 IF　1:800 (frozen section)
Immunogen (Antigen)	KLH conjugated synthetic peptide, MDRICKPDYLPT (corresponding to amino acid residues 159-170 of zebrafish gnat2)
Reactivity [Gene ID]	Zebrafish[140429]
Storage buffer	PBS/50% glycerol, pH 7.2
Storage temp.	-20°C
Conjugate	Unlabeled
Manufacturer	MBL
Alternative names	guanine nucleotide binding protein (G protein), alpha transducing activity polypeptide 2
Background	Transducin, also known as Gt, is a heterotrimeric guanine nucleotide-binding protein with three polypeptide chains: alpha, beta, and gamma. Gt is expressed in rods and cones of retinal photpreceptors. Transducin alpha-subunits are different in rods and cones, being encoded by different genes, GNAT1 in rods and GNAT2 in cones respectively. GNAT2 plays an important role in the light-signalling pathway in the retinal cone cells and also associates with synchronization of circadian rhythm in vertebrates including Zebrafish and Xenopus, as well as mouse and human.
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Product category	Research area:Neuroscience Circadian rhythm
Data
Citations	Western Blotting Yu M et al. Eyes shut homolog is required for maintaining the ciliary pocket and survival of photoreceptors in zebrafish. Biol Open 5, 1662-1673 (2016)(PMID:27737822) Immunohistochemistry Yi Z et al. Comparative exome sequencing reveals novel candidate genes for retinitis pigmentosa. EBioMedicine. 56, 102792 (2020)(PMID:32454406) Immunofluorescence Ogawa Y et al. Homeobox transcription factor Six7 governs expression of green opsin genes in zebrafish. Proc Biol Sci. 282, 20150659 (2015)(PMID:26180064) Yu M et al. Eyes shut homolog is required for maintaining the ciliary pocket and survival of photoreceptors in zebrafish. Biol Open 5, 1662-1673 (2016)(PMID:27737822) Corral-Serrano JC et al. C2orf71a/pcare1 is important for photoreceptor outer segment morphogenesis and visual function in zebrafish. Sci Rep. 8, 9675 (2018)(PMID:29946172) Liu Y et al. Eyes shut homolog (EYS) interacts with matriglycan of O-mannosyl glycans whose deficiency results in EYS mislocalization and degeneration of photoreceptors. Sci Rep. 10, 7795 (2020)(PMID:32385361)
References	Brockerhoff SE et al. Light stimulates a transducin-independent increase of cytoplasmic Ca2+ and suppression of current in cones from the zebrafish mutant nof. J Neurosci. 23, 470-80 (2003)

The availability is based on the information in Japan at 17:00, Apr 24, 2024 in JST.
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Abbreviations for applications：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
For applications and reactivity:
*: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
**: The use is reported from the licenser (Under evaluation or not tested by MBL).
For storage temparature: RT: room temparature
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