Antibody detailed product information

Code No.	PM059
Anti-KDEL pAb
Size	100 µL
Availability (in Japan)	1-9 （In Japan at 00:05, Apr 19, 2024 in JST）
Clonality	Polyclonal
Clone	Polyclonal
Isotype (Immunized Animal)	Rabbit Ig (aff.)
Applications	WB　1:500 IC　1:1,000 IH*　reported. （PMID: 19767744） IF*　reported. （PMID: 28342860）
Immunogen (Antigen)	Mouse GRP78 C-terminal 644-654 aa
Reactivity [Gene ID]	Human, Mouse
Storage buffer	PBS/50% glycerol, pH 7.2
Storage temp.	-20°C
Conjugate	Unlabeled
Manufacturer	MBL
Background	The KDEL (lys-asp-glu-leu) sequence is most common endoplasmic reticulum (ER) retention signal. The ER resident proteins, which should be located in the ER, have this retention motif, and this retention is mediated by KDEL receptor. This antibody can specifically detect GRP78 (78 kDa protein) and GRP94 (94 kDa protein) including a KDEL sequence by WB.
Related products	M178-3 Anti-Calnexin mAb PM060 Anti-Calnexin pAb M176-3 Anti-EEA1 mAb PM062 Anti-EEA1 pAb M179-3 Anti-GM130 mAb PM061 Anti-GM130 pAb M181-3 Anti-KDEL mAb PM064 Anti-Lamin B1 pAb PM063 Anti-COX4 pAb
Product category	Tools:Control antibodies Others
Data
Citations	Immunocytochemistry Ishii S et al. Functional characterization of lysophosphatidic acid receptor 1 mutants identified in rat cancer tissues. Biochem Biophys Res Commun. 486, 767-773 (2017)(PMID:28342860) Kouga T et al. Drug screening for Pelizaeus-Merzbacher disease by quantifying the total levels and membrane localization of PLP1. Mol Genet Metab Rep. 20, 100474 (2019)(PMID:31110947) Yamanaka T et al. Proteomics-Based Approach Identifies Altered ER Domain Properties by ALS-Linked VAPB Mutation. Sci Rep. 10, 7610 (2020)(PMID:32376919) Immunohistochemistry Saito A et al. Regulation of endoplasmic reticulum stress response by a BBF2H7-mediated Sec23a pathway is essential for chondrogenesis. Nat Cell Biol. 11, 1197-1204 (2009)(PMID:19767744) Yamanaka T et al. NF-Y inactivation causes atypical neurodegeneration characterized by ubiquitin and p62 accumulation and endoplasmic reticulum disorganization. Nat Commun. 5, 3354 (2014)(PMID:24566496) Yamanaka T et al. Differential roles of NF-Y transcription factor in ER chaperone expression and neuronal maintenance in the CNS. Sci Rep. 6, 34575 (2016)(PMID:27687130)

The availability is based on the information in Japan at 00:05, Apr 19, 2024 in JST.
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Abbreviations for applications：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
For applications and reactivity:
*: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
**: The use is reported from the licenser (Under evaluation or not tested by MBL).
For storage temparature: RT: room temparature
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