Antibody detailed product information

Code No. PM048
Anti-Oct3/4 pAb
Size 100 µL
Availability (in Japan) 1-9
（In Japan at 00:05, Apr 24, 2024 in JST）
Clonality Polyclonal
Clone polyclonal
Isotype
(Immunized Animal) 		Rabbit Ig (aff.)
Applications WB　1:1,000  
IP　2 µL/300 µL of cell extract from 3 x 106 cells  
IC　
IH　1:500  
Immunogen
(Antigen)		 Recombinant Mouse Oct3/4 N-terminal （1-134 aa)
Reactivity [Gene ID]

Human[5460], Mouse[18999]
Storage buffer PBS/50% glycerol, pH 7.2
Storage temp. -20°C
Conjugate Unlabeled
Manufacturer MBL
Alternative names POU5F1, OCT3, OCT4, OTF3, OTF4, OTF-3
Background The POU family transcription factor Oct-3/4, termed as Oct-3 or Oct-4, encoded by Pou5f1, is expressed in totipotent/pluripotent early embryonic cells. It is also expressed in embryonic stem (ES) cells and embryonal carcinoma (EC) cells, but its expression diminishes when these cells differentiate and lose pluripotency. Oct3/4 contains three functionally characterized domains, the transcriptional activation domain of N- and C-terminal region and the POU DNA-binding domain. The POU domain binds to an octamer sequence, ATTTGCAT. Several target genes of Oct3/4, such as Sox2, contains an octamer element capable of binding Oct3/4. These sites are important for transcriptional activity. Induced pluripotent stem (iPS) cells can be generated from mouse embryonic or adult fibroblasts by induction of four factors, Oct3/4, Sox2, c-Myc, and Klf4. Oct3/4 regulates a expression of Tcl1 (T cell lymphoma break point) and Nanog and contributes to cell proliferation and stabilization of cell pluripotency. Two transcription factors Oct3/4 and Sox2 works together to control a transcriptional regulatory network that regulates the expression of other essential genes.
Related products D356-3 Anti-Jmjd1c (Mouse) mAb
PM056 Anti-Sox2 pAb
PM057 Anti-KLF4 pAb
PM058 Anti-Nanog (Mouse) pAb
Product category Research area:Stem cell research
Data




Citations

Immunocytochemistry

  1. Tsukiyama T, Ohinata Y. A modified EpiSC culture condition containing a GSK3 inhibitor can support germline-competent pluripotency in mice. PLoS One 9, e95329 (2014)(PMID:24736627)
  2. Oura S et al. Chimeric analysis with newly established EGFP/DsRed2-tagged ES cells identify HYDIN as essential for spermiogenesis in mice. Exp Anim. (2018) In press.(PMID:30089752)

Immunohistochemistry

  1. Watanabe Y et al. Notch and Hippo signaling converge on Strawberry Notch 1 (Sbno1) to synergistically activate Cdx2 during specification of the trophectoderm. Sci Rep. 7, 46135 (2017)(PMID:28401892)
  • The availability is based on the information in Japan at 00:05, Apr 24, 2024 in JST.
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  • Abbreviations for applications：
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
    DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
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