Code No. | PM039 | |
Anti-Atg7 (Human) pAb | ||
Size | 100 µL | |
Availability (in Japan) | 1-9
(In Japan at 17:33, Apr 26, 2024 in JST) |
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Clonality | Polyclonal | |
Clone | Polyclonal | |
Isotype (Immunized Animal) |
Rabbit Ig (aff.) | |
Applications | WB 1:1,000-1:2,000 IP 5 µL/300 µL of cell extract from 3x106 cells |
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Immunogen (Antigen) |
Human Atg7 C-terminal synthetic peptide | |
Reactivity [Gene ID] | Human[10533], Mouse(-), Rat(-), Hamster(-) |
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Storage buffer | PBS/50% glycerol, pH 7.2 | |
Storage temp. | -20°C | |
Conjugate | Unlabeled | |
Manufacturer | MBL | |
Alternative names | autophagy related 7, GSA7, APG7L, APG7-LIKE | |
Background | Autophagy is a process of intracellular bulk degradation in which cytoplasmic components including organelles are sequestered within double-membrane vesicles that deliver the contents to the lysosome/vacuole for degradation. Microtubule-associated protein light chain 3 (LC3) is a homologue of yeast Atg8, an essential component of autophagy. Following synthesis, the C-terminus of LC3 is cleaved by a cysteine protease-Atg4, to produce LC3-I, which is located in cytosolic fraction. LC3-I is activated by the E1-like enzyme Atg7 and forms a Atg7-LC3-I thioester. Atg7-LC3-I is transferred to Atg3 to form Atg3-LC3-I thioester. Atg3 is an E2-like enzyme that catalyzes the conjugation of LC3-I and phosphatidylethanolamine (PE) to form LC3-II. The LC3-II-PE conjugate is essential for binding tightly to autophagosomal membrane. | |
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Product category | Research area:Autophagy | |
Data | ||
Citations | Western Blotting
Immunofluorescence
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